A SDS/Page/Western Blot/EIA Protocol for the Specific Detection of Shrimp Viral Pathogens

Loh, Philip C.; Tapay, Lourdes M.; Cesar, E.; Nadala, Cesar; Lu, Yuanan

doi:10.1007/978-1-4757-5983-9_63

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…However, the inhibition of neoplastic transformations in decapods has possibly interfered with the creation of continuous shrimp cell lines so far [13,[43][44][45][46][47]. Indeed, in vivo infection models continue to be an important and useful tool for identifying and studying the pathogenicity of WSSV and for evaluating the efficacy of mitigation strategies to prevent WSSV outbreaks [48][49][50][51][52]. Fundamentally, a WSSV challenge procedure requires the following: (1) the use of animals with low genetic variability and high susceptibility to the virus, free of specific pathogens, (2) a WSSV stock with a known titer of infection, and (3) controlled environmental factors, such as salinity and temperature [51,53].…”

Section: Introductionmentioning

confidence: 99%

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Cox,

De Swaef,

Corteel

et al. 2024

Viruses

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White spot syndrome virus (WSSV) is marked as one of the most economically devastating pathogens in shrimp aquaculture worldwide. Infection of cultured shrimp can lead to mass mortality (up to 100%). Although progress has been made, our understanding of WSSV’s infection process and the virus–host–environment interaction is far from complete. This in turn hinders the development of effective mitigation strategies against WSSV. Infection models occupy a crucial first step in the research flow that tries to elucidate the infectious disease process to develop new antiviral treatments. Moreover, since the establishment of continuous shrimp cell lines is a work in progress, the development and use of standardized in vivo infection models that reflect the host–pathogen interaction in shrimp is a necessity. This review critically examines key aspects of in vivo WSSV infection model development that are often overlooked, such as standardization, (post)larval quality, inoculum type and choice of inoculation procedure, housing conditions, and shrimp welfare considerations. Furthermore, the usefulness of experimental infection models for different lines of WSSV research will be discussed with the aim to aid researchers when choosing a suitable model for their research needs.

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Section: Introductionmentioning

confidence: 99%

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Cox,

De Swaef,

Corteel

et al. 2024

Viruses

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Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

You

Nadala²,

Yang³

et al. 2002

Dis. Aquat. Org.

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A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.KEY WORDS: White spot syndrome virus · 27.5 kDa structural protein · Rabbit polyclonal antiserumResale or republication not permitted without written consent of the publisher

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A SDS/Page/Western Blot/EIA Protocol for the Specific Detection of Shrimp Viral Pathogens

Cited by 2 publications

References 5 publications

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

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