A SCYL2 gene from Oryza sativa is involved in phytosterol accumulation and regulates plant growth and salt stress

Xu, Minyan; Ni, Ying; Tu, Yaling; Wang, Yanping; Zhang, Zhi; Jiao, Yuhuan; Zhang, Xin

doi:10.1016/j.plantsci.2024.112062

Cited by 1 publication

(3 citation statements)

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“…In a prior study, three homozygous transgenic lines overexpressing OsSCYL2 were characterized [ 53 ], serving as the experimental material for subsequent investigations. Seed germination assays were conducted to assess the functional role of OsSCYL2 .…”

Section: Resultsmentioning

confidence: 99%

“…Japonica) were employed as wild-type and the recipients for genetic transformation in the present study. The OsSCYL2 -pCAMBIA1301 vector was constructed by integrating the full-length coding sequence (CDS) of the cloned OsSCYL2 into the pCAMBIA1301 vector, which was subsequently introduced into rice callus and Arabidopsis through Agrobacterium -mediated transformation [ 53 , 59 ]. All primers used for qRT-PCR and vector construction are presented in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…In a prior study, three homozygous transgenic lines overexpressing OsSCYL2 we characterized [53], serving as the experimental material for subsequent investigation Seed germination assays were conducted to assess the functional role of OsSCYL2. T findings revealed a significantly accelerated germination rate in OsSCYL2OE-1, -2, and compared to the wild-type (WT) (Figure 2A,B).…”

Section: Osscyl2 Affects the Speed Of Seed Germinationmentioning

confidence: 99%

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OsSCYL2 is Involved in Regulating ABA Signaling-Mediated Seed Germination in Rice

Xu,

Zhang,

Jiao

et al. 2024

Plants

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Seed germination represents a multifaceted biological process influenced by various intrinsic and extrinsic factors. In the present study, our investigation unveiled the regulatory role of OsSCYL2, a gene identified as a facilitator of seed germination in rice. Notably, the germination kinetics of OsSCYL2-overexpressing seeds surpassed those of their wild-type counterparts, indicating the potency of OsSCYL2 in enhancing this developmental process. Moreover, qRT-PCR results showed that OsSCYL2 was consistently expressed throughout the germination process in rice. Exogenous application of ABA on seeds and seedlings underscored the sensitivity of OsSCYL2 to ABA during both seed germination initiation and post-germination growth phases. Transcriptomic profiling following OsSCYL2 overexpression revealed profound alterations in metabolic pathways, MAPK signaling cascades, and phytohormone-mediated signal transduction pathways, with 15 genes related to the ABA pathways exhibiting significant expression changes. Complementary in vivo and in vitro assays unveiled the physical interaction between OsSCYL2 and TOR, thereby implicating OsSCYL2 in the negative modulation of ABA-responsive genes and its consequential impact on seed germination dynamics. This study elucidated novel insights into the function of OsSCYL2 in regulating the germination process of rice seeds through the modulation of ABA signaling pathways, thereby enhancing the understanding of the functional significance of the SCYL protein family in plant physiological processes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Osscyl2 Affects the Speed Of Seed Germinationmentioning

confidence: 99%

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