A script for initiating molecular biology studies with non-conventional yeasts based on Saccharomycopsis schoenii

Kayacan, Yeseren; Griffiths, Adam; Wendland, Jürgen

doi:10.1016/j.micres.2019.126342

Cited by 9 publications

(11 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…fibuligera . Thus, these fungi have a high potential for application in the fermentation industry, due to their strong amylolytic activity, secretion of amylases, acid protease, and β‐glucosidase (Kayacan et al, 2019). Bal et al (2014) identified S .…”

Section: Discussionmentioning

confidence: 99%

“…In Although we have detected Saccharomycopsis species associated with postharvest sour rot on fruit, the literature reports some species found in traditional fermented beverages, such as S. fibuligera. Thus, these fungi have a high potential for application in the fermentation industry, due to their strong amylolytic activity, secretion of amylases, acid protease, and β-glucosidase (Kayacan et al, 2019). Bal et al (2014) identified S. fibuligera in nuruk, the Korean starter culture used to make various types of Korean alcoholic beverages, including takju, cheongju, and soju.…”

Section: Pathogenicity Testmentioning

confidence: 99%

See 1 more Smart Citation

Molecular phylogeny and morphological characterization of the aetiological agent of sour rot on fruits and vegetables in Brazil

et al. 2021

View full text Add to dashboard Cite

Geotrichum (synonym Galactomyces) is a fungal genus with dimorphic colonial morphotypes (cream-coloured yeast-like form or mould-like colonies) included in the family Dipodascaceae within the Saccharomycetales (Alper et al., 2011;Kurtzman & Robnett, 2013). Geotrichum species are found in many substrates, including soil, plants, milk, and water, or associated with insects (Alper et al., 2011;Hoog & Smith, 2004). Some species are important postharvest plant pathogens of fruits and vegetables that cause economic losses in agriculture worldwide, due to the occurrence of sour rot in stored fruits and vegetables (Agrios, 2005;Hoog & Smith, 2004).These fungi infect fruits and vegetables through injuries caused by insects or mechanical wounds. The symptoms initially appear as water-soaked and soft areas accompanied by a white-to-cream fungal mycelium growth on the surface tissues and a sour odour (Agrios, 2005).Geotrichum is characterized based on the morphology of conidia (arthroconidia), which are formed in chains by the random fragmentation of the hyphae. Species are difficult to distinguish due to the

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Pathogenicity Testmentioning

confidence: 99%

Molecular phylogeny and morphological characterization of the aetiological agent of sour rot on fruits and vegetables in Brazil

et al. 2021

View full text Add to dashboard Cite

show abstract

“…E1 is a diploid yeast strain. Thus, two rounds of PCR-based gene targeting were performed to delete both alleles of FOT1 and FOT2 using two dominant marker genes YES1 (provides G418-resistance) and YES3 (provides clonNAT-resistance) [ 60 ]. Generated mutants grew in the presence of both antibiotics, had correctly integrated both marker genes and had lost FOT1 and FOT2 ORFs ( Figure S2 ).…”

Section: Methodsmentioning

confidence: 99%

Mosaic Genome of a British Cider Yeast

Bernardi

Michling

Fröhlich

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Hybrid formation and introgressions had a profound impact on fermentative yeasts domesticated for beer, wine and cider fermentations. Here we provide a comparative genomic analysis of a British cider yeast isolate (E1) and characterize its fermentation properties. E1 has a Saccharomyces uvarum genome into which ~102 kb of S. eubayanus DNA were introgressed that replaced the endogenous homologous 55 genes of chromosome XIV between YNL182C and YNL239W. Sequence analyses indicated that the DNA donor was either a lager yeast or a yet unidentified S. eubayanus ancestor. Interestingly, a second introgression event added ~66 kb of DNA from Torulaspora microellipsoides to the left telomere of SuCHRX. This region bears high similarity with the previously described region C introgression in the wine yeast EC1118. Within this region FOT1 and FOT2 encode two oligopeptide transporters that promote improved nitrogen uptake from grape must in E1, as was reported for EC1118. Comparative laboratory scale grape must fermentations between the E1 and EC1118 indicated beneficial traits of faster consumption of total sugars and higher glycerol production but low acetic acid and reduced ethanol content. Importantly, the cider yeast strain produced high levels of fruity ester, including phenylethyl and isoamyl acetate.

show abstract

“…To test for promoter activity, the LacZ ORF was placed under the control of H. uvarum promoters using primers 7/8 (HuTEF1-promoter), 9/10 (HuFBA1-promoter), and 11/12 (HuPGK1-promoter), which added 40 bp of flanking homology region to pLacZ (Figure 1). The upstream region of the LacZ ORF [41] was removed by restriction digestion of pLacZ with KpnI and XhoI. The linearised vector and H. uvarum promoters (HuTEF1, HuFBA1, and HuPGK1) were transformed into yeast and fused to LacZ by in vivo recombination.…”

Section: Plasmid Design and Constructionmentioning

confidence: 99%

Development of Genetic Modification Tools for Hanseniasporauvarum

Badura

Wyk

Brezina

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Apiculate yeasts belonging to the genus Hanseniaspora are commonly isolated from viticultural settings and often dominate the initial stages of grape must fermentations. Although considered spoilage yeasts, they are now increasingly becoming the focus of research, with several whole-genome sequencing studies published in recent years. However, tools for their molecular genetic manipulation are still lacking. Here, we report the development of a tool for the genetic modification of Hanseniaspora uvarum. This was employed for the disruption of the HuATF1 gene, which encodes a putative alcohol acetyltransferase involved in acetate ester formation. We generated a synthetic marker gene consisting of the HuTEF1 promoter controlling a hygromycin resistance open reading frame (ORF). This new marker gene was used in disruption cassettes containing long-flanking (1000 bp) homology regions to the target locus. By increasing the antibiotic concentration, transformants were obtained in which both alleles of the putative HuATF1 gene were deleted in a diploid H. uvarum strain. Phenotypic characterisation including fermentation in Müller-Thurgau must showed that the null mutant produced significantly less acetate ester, particularly ethyl acetate. This study marks the first steps in the development of gene modification tools and paves the road for functional gene analyses of this yeast.

show abstract

A script for initiating molecular biology studies with non-conventional yeasts based on Saccharomycopsis schoenii

Cited by 9 publications

References 53 publications

Molecular phylogeny and morphological characterization of the aetiological agent of sour rot on fruits and vegetables in Brazil

Molecular phylogeny and morphological characterization of the aetiological agent of sour rot on fruits and vegetables in Brazil

Mosaic Genome of a British Cider Yeast

Development of Genetic Modification Tools for Hanseniasporauvarum

Contact Info

Product

Resources

About