A screening method for gastric cancer by oral microbiome detection

Sun, Jinghua; Li, Xin; Yin, Jie; Li, Yihong; Hou, Benxiang; Zhang, Zhongtao

doi:10.3892/or.2018.6286

Cited by 53 publications

(71 citation statements)

References 22 publications

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“…Gastric cancer (GC) ranks as the second most prevalent malignancy worldwide and has the fourth highest morbidity rate [1,2]. The incidence of GC differs heterogeneously in different countries [3].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway

Lin

Wang

et al. 2019

Cell Cycle

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Gastric cancer (GC) is one of the major malignancies worldwide. This study was conducted to explore the mechanism by which GREM2 maintains biological properties of GC stem cells (GCSCs), and proved that GREM2 could potentially regulate the proliferation, apoptosis, invasion, migration and tumorigenic ability of GCSCs through the regulation of the JNK signaling pathway. In silico analysis was utilized to retrieve expression microarray related to GC, and differential analysis was conducted. The cell line with the highest GREM2 expression was overexpressed with GREM2 mimic, silencing GREM2 by siRNA, or treated with activator or inhibitor of the JNK signaling pathway. Subsequently, expression of GREM2, JNK signaling pathway-, apoptosis-or migration and invasion-associated factors were determined. Proliferation, migration, invasion, apoptosis of GCSCs in vitro and tumorigenic ability and lymph node metastasis of GCSCs in vivo were determined. Based on the in silico analysis of GSE49051, GREM2 was determined to be overexpressed in GC and its expression was the highest in the MKN-45 cell line, which was selected for the subsequent experiments. Silencing of GREM2 or inhibition of the JNK signaling pathway suppressed the proliferation, migration and invasion, while promoting apoptosis of GCSCs in vitro as well as inhibiting tumorigenesis and lymph node metastasis in vivo. In conclusion, the aforementioned findings suggest that the silencing of GREM2 suppresses the activation of the JNK signaling pathway, thereby inhibiting tumor progression. Therefore, GREM2-mediated JNK signaling pathway was expected to be a new therapeutic strategy for GC.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway

Lin

Wang

et al. 2019

Cell Cycle

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“…We have also presented examples of this approach in Table 1. For instance, Chen et al observed an overall decrease of microbial diversity in the saliva of esophageal cancer patients (67), while an overall increase in diversity in saliva and plaque from gastric cancer patients was observed by Sun et al (95). Except for CRC, where Fusobacterium spp.…”

Section: Oral Microorganisms and Cancers Outside The Oral Cavitymentioning

confidence: 95%

The Oral Microbiome and Cancer

2020

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There is mounting evidence that members of the human microbiome are highly associated with a wide variety of cancer types. Among oral cancers, oral squamous cell carcinoma (OSCC) is the most prevalent and most commonly studied, and it is the most common malignancy of the head and neck worldwide. However, there is a void regarding the role that the oral microbiome may play in OSCC. Previous studies have not consistently found a characteristic oral microbiome composition associated with OSCC. Although a direct causality has not been proven, individual members of the oral microbiome are capable of promoting various tumorigenic functions related to cancer development. Two prominent oral pathogens, Porphyromonas gingivalis, and Fusobacterium nucleatum can promote tumor progression in mice. P. gingivalis infection has been associated with oro-digestive cancer, increased oral cancer invasion, and proliferation of oral cancer stem cells. The microbiome can influence the evolution of the disease by directly interacting with the human body and significantly altering the response and toxicity to various forms of cancer therapy. Recent studies have shown an association of certain phylogenetic groups with the immunotherapy treatment outcomes of certain tumors. On the other side of the coin, recently it has been a resurgence in interest on the potential use of bacteria to cure cancer. These kinds of treatments were used in the late nineteenth and early twentieth centuries as the first line of defense against cancer in some hospitals but later displaced by other types of treatments such as radiotherapy. Currently, organisms such as Salmonella typhimurium and Clostridium spp. have been used for targeted strategies as potential vectors to treat cancer. In this review, we briefly summarize our current knowledge of the role of the oral microbiome, focusing on its bacterial fraction, in cancer in general and in OSCC more precisely, and a brief description of the potential use of bacteria to target tumors.

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“…Similarly, pancreatic carcinoma is insidious, very aggressive and in most cases diagnosed at a very late stage, being associated to a very poor prognosis (46). With regard to gastric cancer 7 studies were included in the present review (15)(16)(17)25,(47)(48)(49). All of these reported statistically significant results.…”

Section: Discussionmentioning

confidence: 99%

“…Molecules most frequently investigated were proteins and RNA. Twelve studies (23.5%) were scored as "good" and 11 of these reported statistically significant associations between molecules searched and pathology (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Inclusion Criteria Exclusion Criteriamentioning

confidence: 99%

Salivary biomarkers for diagnosis of systemic diseases and malignant tumors. A systematic review

Meleti¹,

Cassi²,

Vescovi³

et al. 2020

Med Oral

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Background: Saliva evaluation could be a possible alternative to blood and/or tissue analyses, for researching specific molecules associated to the presence of systemic diseases and malignancies. The present systematic review has been designed in order to answer to the question "are there significant associations between specific salivary biomarkers and diagnosis of systemic diseases or malignancies?". Materials and Methods: The Preferred Reporting Item for Systematic Reviews and Meta-analysis (PRISMA) statement was used to guide the review. The combinations of "saliva" and "systemic diseases" or "diagnosis" or "biomarkers" or "cancers" or "carcinoma" or "tumors", were used to search Medline, Scopus and Web of Science databases. Endpoint of research has been set at May 2019. Studies were classified into 3 groups according to the type of disease investigated for diagnosis: 1) malignant tumors; 2) neurologic diseases and 3) inflammatory/metabolic/cardiovascular diseases. Assessment of quality has been assigned according to a series of questions proposed by the National Institute of Health. Level of evidence was assessed using the categories proposed in the Oxford Center for Evidence-Based medicine (CEMB) levels for diagnosis (2011).

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A screening method for gastric cancer by oral microbiome detection

Cited by 53 publications

References 22 publications

RETRACTED ARTICLE: GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway

RETRACTED ARTICLE: GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway

The Oral Microbiome and Cancer

Salivary biomarkers for diagnosis of systemic diseases and malignant tumors. A systematic review

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