A "screened" electrostatic ion trap for enhanced mass resolution, mass accuracy, reproducibility, and upper mass limit in Fourier-transform ion cyclotron resonance mass spectrometry

Wang, Mingda.; Marshall, Alan G.

doi:10.1021/ac00186a021

Cited by 113 publications

(59 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various solutions have been proposed to deal with the axial trapping field (21,22). In the late 1980s, Wang and Marshall used a grounded mesh just in front of the axial trapping electrodes (23). In this case, the trapping field penetrating through the grid can have an effect only when the ions get very close to the trapping electrodes.…”

Section: Fig 7 Ion Motion Within An Icr Cellmentioning

confidence: 99%

Fourier Transform Mass Spectrometry

Ščigelová

Hornshaw

Giannakopulos

et al. 2011

Molecular & Cellular Proteomics

182

137

View full text Add to dashboard Cite

Mass spectrometers have been used for a long time in a variety of biological applications. Recent years, however, have witnessed a significant increase in the employment of mass spectrometers such as of time-of-flight, Fourier transform ion cyclotron resonance (FTICR) 1 , and Orbitrap, which provide accurate mass of analytes over wide mass range. The FTICR and Orbitrap analyzers outperform any other commonly used mass spectrometer with respect to the maximum mass resolution and accuracy routinely achievable even for small numbers of ions. Both these mass spectrometers share certain features, such as an image current detection system and the application of Fourier transform mathematical operations for generating mass spectra from time domain transients produced by the image current. Consequently, they are often referred to as Fourier transform-based mass spectrometers (FTMS).The combination of FTMS with ion preselection and fragmentation devices and their coupling to reversed-phase liquid chromatography (LC) represents a ubiquitous approach to both small molecule and proteomic analyses. Multidimensional LC separations have an important role to play in proteomics applications for reducing sample complexity, and complement well the high dynamic range of detection in an acquisition offered by FTMS instruments.The current article does not intend to be an exhaustive review paper on FTMS techniques; it is intended as teaching material for scientists without a physics background to assist them with understanding the mass spectrometry tools they are called to use in their everyday laboratory work. Thus, only the fundamental aspects of FTMS which are useful to a nonphysicist are introduced. The discussion then focuses on examples of where this technology can be applied and what are the benefits as well as limitations of this technology for practical proteomic applications.Need for High Resolution and Mass Accuracy-The mass accuracy is the ratio of the m/z measurement error to the true m/z, usually quoted in parts per million (ppm). The mass resolving power (resolution) is the measure of the ability to distinguish two peaks of slightly different m/z, herein understood as full width at half maximum (FWHM). Linearity of detection and very high fidelity in the determination of frequency are inherent to FTMS and allow very high resolving power, mass accuracy, and dynamic range to be achieved. But why would one need high mass accuracy and high resolution?The benefit of measuring a compound's mass with adequately high accuracy can directly determine its elemental composition. Accurate mass thus acts as a powerful "filter" useful for confirming the identity of a compound or even identification of an unknown. This is illustrated in Fig. 1 showing the fragmentation (tandem mass spectrometry (MS/MS)) spectrum of flavonoid quercetin (m/z 303). Mass deviation of less than 3 ppm for any detected fragment together with the richness of the fragmentation spectra itself enable confirming the elemental composition of the starting compound a...

show abstract

Section: Fig 7 Ion Motion Within An Icr Cellmentioning

confidence: 99%

Fourier Transform Mass Spectrometry

Ščigelová

Hornshaw

Giannakopulos

et al. 2011

Molecular & Cellular Proteomics

182

137

View full text Add to dashboard Cite

show abstract

“…Ions experience a restoring force in the axial direction only at the axial-boundaries of the cell. Although it is extremely difficult to form the sharp field gradients at the edges of the cell, Wang and Marshall (66) reported that the particle-in-a-box potential is approximated in a cubic cell by mounting grounded screens in front of the trapping plates. When the performance of a screened orthorhombic cell (6.4 cm X 5.08 cm X 5.08 cm), was compared to that of an unscreened cell, the authors found that the screens attenuated the ion cyclotron-frequency shift due to the trapping voltage by a factor of one hundred and reduced the variation of cyclotron frequency with increasing ion-orbits radius by a factor of 10-20 times.…”

Section: Particle-in-a-box Ideal: Elimination Of the Radial Fieldmentioning

confidence: 99%

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Holliman

Rempel

Gross

1994

Mass Spectrometry Reviews

View full text Add to dashboard Cite

“…The first one is to reduce a radial electric field in the region of ion detection [5][6][7][8]. The second one is to create an electric field configuration that leaves the cyclotron frequency of ions independent of their axial motion-that is a hyperbolic field:…”

Section: Introductionmentioning

confidence: 99%

Initial Experimental Characterization of a New Ultra-High Resolution FTICR Cell with Dynamic Harmonization

Nikolaev

Boldin

Jertz

et al. 2011

J. Am. Soc. Mass Spectrom.

145

157

View full text Add to dashboard Cite

A new Fourier transform ion cyclotron resonance (FTICR) cell based on completely new principles of formation of the effective electric potential distribution in Penning type traps, Boldin and Nikolaev (Proceedings of the 58th ASMS Conference, 2010), Boldin and Nikolaev (Rapid Commun Mass Spectrom 25:122-126, 2011) is constructed and tested experimentally. Its operation is based on the concept of electric potential space-averaging via charged particle cyclotron motion. Such an averaging process permits an effective electric force distribution in the entire volume of a cylindrical Penning trap to be equal to its distribution in the field created by hyperbolic electrodes in an ideal Penning trap. The excitation and detection electrodes of this new cell are shaped for generating a quadratic dependence on axial coordinates of an averaged (along cyclotron motion orbit) electric potential at any radius of the cyclotron motion. These electrodes together with the trapping segments form a cylindrical surface like in a conventional cylindrical cell. In excitation mode this cell being elongated behaves almost like an open cylindrical cell of the same length. It is more effective in ion motion harmonization at larger cyclotron radii than a Gabrielse et al.-type (Int J Mass Spectrom Ion Processes 88:319-332, 1989) cylindrical cell with four compensation sections. A mass resolving power of more than twenty millions of reserpine (m/z 609) and more than one million of highly charged BSA molecular ions (m/z 1357) has been obtained in a 7T magnetic field.

show abstract

A "screened" electrostatic ion trap for enhanced mass resolution, mass accuracy, reproducibility, and upper mass limit in Fourier-transform ion cyclotron resonance mass spectrometry

Cited by 113 publications

References 29 publications

Fourier Transform Mass Spectrometry

Fourier Transform Mass Spectrometry

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Initial Experimental Characterization of a New Ultra-High Resolution FTICR Cell with Dynamic Harmonization

Contact Info

Product

Resources

About