A screen for Neurospora knockout mutants displaying growth rate dependent branch density

Watters, Michael K.; Boersma, Michael J.; Johnson, Melodie; Reyes, Ciara; Westrick, Evan; Lindamood, Erik

doi:10.1016/j.funbio.2010.12.015

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…Kawano and Said [56] found that an excess of Ca 2+ acts as an environmental suppressor of hyperbranching under several circumstances. Watters and all [57] found that a diverse gene network coordinates branching. Hyperbranching could also be an effect of spaceflight on disruption of genes involved in controlling branching.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Spaceflight on Growth of Ulocladium chartarum Colonies on the International Space Station

et al. 2013

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The objectives of this 14 days experiment were to investigate the effect of spaceflight on the growth of Ulocladium chartarum, to study the viability of the aerial and submerged mycelium and to put in evidence changes at the cellular level. U. chartarum was chosen for the spaceflight experiment because it is well known to be involved in biodeterioration of organic and inorganic substrates covered with organic deposits and expected to be a possible contaminant in Spaceships. Colonies grown on the International Space Station (ISS) and on Earth were analysed post-flight. This study clearly indicates that U. chartarum is able to grow under spaceflight conditions developing, as a response, a complex colony morphotype never mentioned previously. We observed that spaceflight reduced the rate of growth of aerial mycelium, but stimulated the growth of submerged mycelium and of new microcolonies. In Spaceships and Space Stations U. chartarum and other fungal species could find a favourable environment to grow invasively unnoticed in the depth of surfaces containing very small amount of substrate, posing a risk factor for biodegradation of structural components, as well as a direct threat for crew health. The colony growth cycle of U. chartarum provides a useful eukaryotic system for the study of fungal growth under spaceflight conditions.

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Section: Discussionmentioning

confidence: 99%

The Effect of Spaceflight on Growth of Ulocladium chartarum Colonies on the International Space Station

et al. 2013

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“…They are regulated differentially during different growth stages (22,109,124). Mutants lacking the cat-1 or cat-3 gene show a temperature-dependent increase in the branch density of the growing hyphae, whereas the branch density of wt is independent of the temperature (171). Involvement of CAT-3 in the morphogenesis of hyphae is also supported by results showing that the deletion of cat-3 rescues the morphological change of the hyphae in a mutant lacking nucleoside diphosphate kinase-1 (87).…”

Section: Ros Homeostasis Progressively Changes During Cell Differentimentioning

confidence: 99%

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Gyöngyösi

Káldi

2014

Antioxidants & Redox Signaling

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Significance: Both circadian rhythm and the production of reactive oxygen species (ROS) are fundamental features of aerobic eukaryotic cells. The circadian clock enhances the fitness of organisms by enabling them to anticipate cycling changes in the surroundings. ROS generation in the cell is often altered in response to environmental changes, but oscillations in ROS levels may also reflect endogenous metabolic fluctuations governed by the circadian clock. On the other hand, an effective regulation and timing of antioxidant mechanisms may be crucial in the defense of cellular integrity. Thus, an interaction between the circadian timekeeping machinery and ROS homeostasis or signaling in both directions may be of advantage at all phylogenetic levels. Recent Advances: The Frequency-White Collar-1 and White Collar-2 oscillator (FWO) of the filamentous fungus Neurospora crassa is well characterized at the molecular level. Several members of the ROS homeostasis were found to be controlled by the circadian clock, and ROS levels display circadian rhythm in Neurospora. On the other hand, multiple data indicate that ROS affect the molecular oscillator. Critical Issues: Increasing evidence suggests the interplay between ROS homeostasis and oscillators that may be partially or fully independent of the FWO. In addition, ROS may be part of a complex cellular network synchronizing non-transcriptional oscillators with timekeeping machineries based on the classical transcription-translation feedback mechanism. Future Directions: Further investigations are needed to clarify how the different layers of the bidirectional interactions between ROS homeostasis and circadian regulation are interconnected. Antioxid. Redox Signal. 20,[3007][3008][3009][3010][3011][3012][3013][3014][3015][3016][3017][3018][3019][3020][3021][3022][3023]

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“…It can be influenced by environmental factors like calcium [76], choline [77] or manganese [78] and can be modified by many mutations affecting cell signalling and transcription factors, cytoskeleton and associated motors, cell wall, ubiquitin-binding proteins, metal binding/metabolism proteins, reactive oxygen species control proteins or clock-related proteins [45], [79]–[86]. In addition to the Podospora anserina pah1 gene [58], BcHOX8 is the second homeobox gene whose mutation affects hyphal branching, and whether or how these two genes relates to one of the above functions needs to be clarified.…”

Section: Discussionmentioning

confidence: 99%

The Homeobox BcHOX8 Gene in Botrytis Cinerea Regulates Vegetative Growth and Morphology

et al. 2012

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Filamentous growth and the capacity at producing conidia are two critical aspects of most fungal life cycles, including that of many plant or animal pathogens. Here, we report on the identification of a homeobox transcription factor encoding gene that plays a role in these two particular aspects of the development of the phytopathogenic fungus Botrytis cinerea. Deletion of the BcHOX8 gene in both the B. cinerea B05-10 and T4 strains causes similar phenotypes, among which a curved, arabesque-like, hyphal growth on hydrophobic surfaces; the mutants were hence named Arabesque. Expression of the BcHOX8 gene is higher in conidia and infection cushions than in developing appressorium or mycelium. In the Arabesque mutants, colony growth rate is reduced and abnormal infection cushions are produced. Asexual reproduction is also affected with abnormal conidiophore being formed, strongly reduced conidia production and dramatic changes in conidial morphology. Finally, the mutation affects the fungus ability to efficiently colonize different host plants. Analysis of the B. cinerea genome shows that BcHOX8 is one member of a nine putative homeobox genes family. Available gene expression data suggest that these genes are functional and sequence comparisons indicate that two of them would be specific to B. cinerea and its close relative Sclerotinia sclerotiorum.

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A screen for Neurospora knockout mutants displaying growth rate dependent branch density

Cited by 16 publications

References 38 publications

The Effect of Spaceflight on Growth of Ulocladium chartarum Colonies on the International Space Station

The Effect of Spaceflight on Growth of Ulocladium chartarum Colonies on the International Space Station

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

The Homeobox BcHOX8 Gene in Botrytis Cinerea Regulates Vegetative Growth and Morphology

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