A <scp>CACTA</scp>‐like transposable element in the upstream region of <i>BnaA9</i>.<i><scp>CYP</scp>78A9</i> acts as an enhancer to increase silique length and seed weight in rapeseed

Shi, Liu; Song, Jurong; Guo, Chaocheng; Wang, Bo; Guan, Zhilin; Yang, Pu; Chen, Xun; Zhang, Qinghua; King, Graham J.; Wang, Jing; Liu, Kede

doi:10.1111/tpj.14236

Cited by 86 publications

(74 citation statements)

References 70 publications

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“…Interestingly, many of these genes have a conserved function in regulating fruit development in both dry and fleshy fruit types, suggesting broad applicability of common genome edits. For instance, CYP78A9 and CLV‐WUS pathway genes can regulate both silique size in Arabidopsis /rapeseed (Fan et al , ; Shi et al , ; Xiao et al , ; Xu et al , ; Yang et al , ) and fruit size in cherry/tomato (Lemmon et al , ; Li et al , ; Qi et al , ; Rodriguez‐Leal et al ., ; Xu et al , ). Besides, many of these genes have similar functions in regulating fruit size in Arabidopsis and other crops.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“…Besides, many of these genes have similar functions in regulating fruit size in Arabidopsis and other crops. For example, ARF18 , ARP1 , BoMF2 , DRM1 , CYP78A9 , CLV1 , CLV3 and SMG7 can regulate silique length in Arabidopsis and rapeseed (Kang et al , ; Lee et al , ; Li et al , ; Liu et al , ; Shi et al , ; Xiao et al , ; Yang et al , ). More importantly, many of the fruit size regulatory genes have expression activity and additional effects on other organs, such as roots, stems, leaves, flowers and seeds, indicating a cooperative/synergistic regulation.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

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Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

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Summary Fruit is seed‐bearing structures specific to angiosperm that form from the gynoecium after flowering. Fruit size is an important fitness character for plant evolution and an agronomical trait for crop domestication/improvement. Despite the functional and economic importance of fruit size, the underlying genes and mechanisms are poorly understood, especially for dry fruit types. Improving our understanding of the genomic basis for fruit size opens the potential to apply gene‐editing technology such as CRISPR/Cas to modulate fruit size in a range of species. This review examines the genes involved in the regulation of fruit size and identifies their genetic/signalling pathways, including the phytohormones, transcription and elongation factors, ubiquitin‐proteasome and microRNA pathways, G‐protein and receptor kinases signalling, arabinogalactan and RNA‐binding proteins. Interestingly, different plant taxa have conserved functions for various fruit size regulators, suggesting that common genome edits across species may have similar outcomes. Many fruit size regulators identified to date are pleiotropic and affect other organs such as seeds, flowers and leaves, indicating a coordinated regulation. The relationships between fruit size and fruit number/seed number per fruit/seed size, as well as future research questions, are also discussed.

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Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

Self Cite

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show abstract

“…Additionally, it is interesting to note that auxin also regulates silique development. Overexpression of BnaA9.CYP78A9, which encodes a P450 monooxygenase, induces a significant increase in auxin in developing siliques, stimulating the elongation of siliques in Brassica napus [51].…”

Section: Auxin Levels Influence Seed Developmentmentioning

confidence: 99%

Into the Seed: Auxin Controls Seed Development and Grain Yield

Cao

Guo-ji

et al. 2020

IJMS

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Seed development, which involves mainly the embryo, endosperm and integuments, is regulated by different signaling pathways, leading to various changes in seed size or seed weight. Therefore, uncovering the genetic and molecular mechanisms of seed development has great potential for improving crop yields. The phytohormone auxin is a key regulator required for modulating different cellular processes involved in seed development. Here, we provide a comprehensive review of the role of auxin biosynthesis, transport, signaling, conjugation, and catabolism during seed development. More importantly, we not only summarize the research progress on the genetic and molecular regulation of seed development mediated by auxin but also discuss the potential of manipulating auxin metabolism and its signaling pathway for improving crop seed weight.

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“…TEs are frequent found in B. napus genomes (Chalhoub et al, 2014; Sun et al, 2017) and have been implicated in DNA methylation and H3K9me2 modification (Eichten et al, 2012; Gent et al, 2013), altering gene expression both genetically and epigenetically (Cui and Cao, 2014). In fact, several oilseed rape genes related to morphological or physiological traits have evolved from TE insertions (Hou et al, 2012; Zhang et al, 2015; Gao et al, 2016; Shi et al, 2019). The current study implies that DNA methylation of the Copia-LTR insertion spreads to BnSHP1.A9 cis-regulatory region.…”

Section: Discussionmentioning

confidence: 99%

An Epigenetic LTR-retrotransposon insertion in the upstream region ofBnSHP1.A9controls quantitative pod shattering resistance inBrassica napus

Liu

Zhou

Wang

et al. 2019

Preprint

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Seed loss resulting from pod shattering is a major problem in oilseed rape (Brassica napus L.) production worldwide. However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here we show that the pod shatter resistance at quantitative trait locus, qSRI.A9.1 is controlled by a SHATTERPROOF1 (SHP1) paralog in B. napus (BnSHP1.A9). Expression analysis by quantitative RT-PCR showed that BnSHP1.A9 was specifically expressed in flower buds, flowers and developing siliques in the oilseed rape line (R1) carrying the qSRI.A9.1 allele with negative effect, but not expressed in any tissue of the line (R2) carrying the positive effect qSRI.A9.1 allele. Transgenic plants constitutively expressing BnSHP1.A9 alleles from pod resistant and pod shattering parental lines showed that both alleles are responsible for pod shattering via promoting lignification of enb layer, which indicated allelic difference of BnSHP1.A9 gene per se is not the causal factor of the QTL. The upstream sequence of BnSHP1.A9 in the promotor region harboring highly methylated long terminal repeat retrotransposon insertion (LTR, 4803bp) in R2 repressed the expression of BnSHP.A9, and thus contributed to the positive effect on pod shatter resistance. Genetic and association analysis revealed that the copia LTR retrotransposon based marker BnSHP1.A9-R2 can be used for breeding for pod shatter resistant varieties and reducing the loss of seed yield in oilseed rape.

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A CACTA‐like transposable element in the upstream region of BnaA9.CYP78A9 acts as an enhancer to increase silique length and seed weight in rapeseed

Cited by 86 publications

References 70 publications

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Into the Seed: Auxin Controls Seed Development and Grain Yield

An Epigenetic LTR-retrotransposon insertion in the upstream region ofBnSHP1.A9controls quantitative pod shattering resistance inBrassica napus

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