A scientific note on the characterization of microsatellite loci for<i>Melipona mondury</i>(Hymenoptera: Apidae)

Lopes, Denilce Meneses; Silva, Filipe Oliveira da; Fernandes-Salomão, Tânia M.; Campos, Lúcio Antônio de Oliveira; Tavares, Mara Garcia

doi:10.1051/apido/2009067

Cited by 15 publications

(18 citation statements)

References 11 publications

(10 reference statements)

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“…According to Lopes et al (2010), though heterologous markers can be used, the result must be carefully analyzed due to the presence of null alleles arising from mutations in the annealing sites. Only five species of the genus Melipona have specific microsatellite markers.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 49 pairs of primers described for the species Melipona (Eomelipona) bicolor (Peters et al, 1998), Melipona (Michmelia) rufiventris (Lopes et al, 2009), Melipona (Michmelia) mondury (Lopes et al, 2010), Melipona (Melikerria) interrupta manaosensis (Franciniet al, 2009b), Melipona (Michmelia) seminigra merrillae (Francini et al, 2009a) and Trigona carbonaria (Green et al, 2001) were tested ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…The Melipona genus has the largest number of species, about 70 (Moure et al, 2007), and can be found throughout the Neotropical region, from Mexico to Misiones, Argentina, with highest diversity in the Amazon basin (Camargo andPedro, 1992, Silveira et al, 2002). Until now, microsatellite primers have only been described for five species of this genus: Melipona bicolor (Peters et al, 1998), Melipona rufiventris (Lopes et al, 2009), Melipona seminigra merrillae (Francini et al, 2009a), Melipona interrupta manaosensis (Francini et al, 2009b) and Melipona mondury (Lopes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

Viana¹,

Miranda²,

Francisco³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Melipona has the largest number of species among bee genera, about 70, occurring throughout the Neotropical region. However, only five species of the genus Melipona have specific microsatellite markers. Given the great diversity of this genus, this number is not representative. We analyzed the transferability of 49 microsatellite loci to four other species of the genus Melipona (M. scutellaris, M. mondury, M. mandacaia, and M. quadrifasciata). Four individuals of each species, from different localities, were used in amplification tests. Primer pairs described for five Melipona species and for Trigona carbonaria were tested. Among the 49 loci, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 3942-3947 (2011) Transferability of microsatellite primers among Melipona 3943 22 gave amplification products for all four species, while three gave nonspecific bands and five showed no amplification products. The remaining loci varied in the pattern of amplification, according to the species examined. The number of alleles ranged from 1 to 6. The results demonstrate the possibility of using these heterologous markers in other Melipona species, increasing the number of loci that can be analyzed and contributing to further genetic analyses of intra-and intercolonial structure, which is required for conservation measure planning, genetic improvement and resolution of taxonomic problems.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

Viana¹,

Miranda²,

Francisco³

et al. 2011

Genet. Mol. Res.

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show abstract

“…Higher values (8.94) were only described for the species Tetragonisca angustula (Brito et al 2009). In contrast, studies applying ISSR methodology to isolate species specific microsatellite loci found only low levels of allelic richness, 3 and 2.63 for Melipona mondury and Melipona rufiventris, respectively (Lopes et al 2009(Lopes et al , 2010. Therefore, the methodology of an enriched genomic library seems to be more efficient, even though the low level of Lopes et al (2009Lopes et al ( , 2010 may be related to their sampling strategy.…”

mentioning

confidence: 85%

“…In contrast, studies applying ISSR methodology to isolate species specific microsatellite loci found only low levels of allelic richness, 3 and 2.63 for Melipona mondury and Melipona rufiventris, respectively (Lopes et al 2009(Lopes et al , 2010. Therefore, the methodology of an enriched genomic library seems to be more efficient, even though the low level of Lopes et al (2009Lopes et al ( , 2010 may be related to their sampling strategy. The authors analyzed 20 individuals from a single population of each species, whereas in our study we analyzed 19 unrelated individuals collected in different localities.…”

mentioning

confidence: 85%