A Schedule for the Study of Somatic Chromosomes in Bromus

Schreiber, Jack

doi:10.3109/10520295109113217

Stain Technology

1951

DOI: 10.3109/10520295109113217

|View full text |Cite

A Schedule for the Study of Somatic Chromosomes in Bromus

Jack Schreiber

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1954

1956

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The superiority of the squash method for chromosome studies over microtome sectioning is evidenced by numerous papers (O'Mara, 1939(O'Mara, , 1948Burrell, 1939;Schmuck and Kostoff, 1939;Swanson, 1940;Bhaduri, 1940;Rosen, 1946;McKay and Clarke, 1946;Gerstel, 1949;Tjio and Levan, 1950;Sharma and Ghosh, 1950;Schreiber, 1951, andSharma andBhattacharjee, 1952) that have appeared since it was introduced, with Feulgen staining, by Heitz (1936).…”

mentioning

confidence: 96%

Chromosome squashes in Cereals

Bhaduri

Ghosh

1954

Stain Technology

View full text Add to dashboard Cite

Root tips were soaked 1-2 hours at 18-20" C. in a freshly prepared saturated aqueous solution of a-bromonaphthalene, 1-2 hours in water, and fixed in a mixture consisting of 1% chromic acid, 5 ml.; 2% osmic acid, 1 ml.; and 0.002 M 8-0xyquinoline, 1 ml. for 0.5-1.0 hour at 10-14" C. They were then successively treated as follows: water 1-2 minutes; 1% H,SO,, 10-15 minutes; water, 1-2 minutes; 1% Cr03, 0.5-1.0 hour; water 3-5 minutes; 1 N HCl, 45 minutes at 60" C.; water 3-5 minutes; leucobasic fuchsin (de Tomasi) 0.5-1.0 hour. The brightly stained tips of the roots were cut off and squashed in the usual manner in a drop of aceto-carmine under a cover glass. The cover glass was sealed with paraffin and the slide stored overnight. T h e paraffin was removed and the cover glass separated in a -l : l mixture of acetic acid and n-butyl alcohol. Final processing consisted of passing the slide through pure n-butyl alcohol; a 1:l mixture of redistilled turpentine and n-butyl alcohol (to remove-the osmic acid stain); n-butyl alcohol, 2 changes, and covering in balsam.

show abstract

mentioning

confidence: 96%

Chromosome squashes in Cereals

Bhaduri

Ghosh

1954

Stain Technology

View full text Add to dashboard Cite

show abstract

Fixation of plant chromosomes

Sharma

1956

Bot. Rev

View full text Add to dashboard Cite

Staining Plant and Animal Chromosomes by the Feulgen-Acetocarmine Sequence

Schreiber

1954

Stain Technology

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Schedule for the Study of Somatic Chromosomes in Bromus

Cited by 3 publications

References 0 publications

Chromosome squashes in Cereals

Chromosome squashes in Cereals

Fixation of plant chromosomes

Staining Plant and Animal Chromosomes by the Feulgen-Acetocarmine Sequence

Contact Info

Product

Resources

About