A scalable, micropore, platelet rich plasma separation device

Dickson, Mary Nora; Amar, Levy; Hill, Michael; Schwartz, Joseph; Leonard, Edward F.

doi:10.1007/s10544-012-9675-2

Cited by 15 publications

(6 citation statements)

References 14 publications

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“…They reported a ratio of separated RBCs, platelets, and WBCs as 470:36:1. Dickson et al 20 used a two-step, cross flow filtration microdevice and achieved 115% extraction fraction of platelets using a 3.5 μm pore size filter. Using micropatterned platelet-specific proteins on a glass substrate, Basabe-Desmonts et al 21 selectively captured platelets.…”

Section: Introductionmentioning

confidence: 99%

Separation and Enrichment of Platelets from Whole Blood Using a PDMS-Based Passive Microdevice

Laxmi

Tripathi

Joshi

et al. 2020

Ind. Eng. Chem. Res.

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Separation and enrichment of platelets in blood plasma play a major role in monitoring the human health condition and transfusion purposes. Although centrifugation is frequently employed to achieve platelet separation, recent advancements in the field of cell separation place microfluidic platforms in advantageous positions over the conventional ones. In this work, we report the development of a PDMS-based passive microfluidics device to separate and enrich platelets in blood plasma. The devices were tested with whole blood in a practically relevant flow rate range of 0.2–0.6 mL/min. One of our proposed devices, comprising gradual constriction–expansion, is able to separate and enrich platelets with 8.7-fold enrichment factor, 25.5% purity, and with minimum activation at a flow rate of 0.4 mL/min using whole human blood. The amount of enrichment and purity is found to be sensitive to the ratio of RBCs and WBCs to platelets in the incoming sample. The ability of the microdevice to handle whole blood at a high flow rate, and enrich platelets in plasma with minimal activation, makes the microdevice unique. These results also demonstrate the strong effect of the RBCs and WBCs to platelet ratio on the amount of enrichment and purity that can be obtained from passive microdevices.

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Section: Introductionmentioning

confidence: 99%

Separation and Enrichment of Platelets from Whole Blood Using a PDMS-Based Passive Microdevice

Laxmi

Tripathi

Joshi

et al. 2020

Ind. Eng. Chem. Res.

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“…Meanwhile, the passive microdevices are designed to utilize hydrodynamic effects, geometrical effects of microchannel design, and flow dynamics of the blood cells in the microchannels for their operation. The passive microdevices available in the literature for platelet and plasma separation utilize inertial effects, hydrodynamic effects, − hydrophoretic effects, deterministic lateral displacement, and filtration techniques. − Platelet separation is also reported using platelet-specific protein patterning on a substrate . Despite availability of platelet separation and plasma separation microdevices, only a few facilitate enrichment of platelets in the plasma sample.…”

Section: Introductionmentioning

confidence: 99%

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Laxmi

Joshi

Agrawal

2020

Ind. Eng. Chem. Res.

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Separation and enrichment of platelets from whole blood find numerous applications for biomedical research, dermatology, and transfusion purposes. Therefore, there is a great value in selectively isolating and enriching platelets in plasma from whole blood. The conventional method of centrifugation involves several manual steps and cannot be integrated with point-of-care devices.Here, we present an evolved passive microdevice for the enhanced fold enrichment and separation efficiency of platelets in a platelet-enriched plasma sample. The design steps for arriving at the final design are also presented, as learnings from these early designs helped in evolution of the final microdevice; these early designs can also be used as a reference for further studies. The final proposed microdevice is easy to fabricate, requires less hardware and pumping power than available microdevices, and separates high-quality platelets from a whole blood sample. Our microdevice is able to achieve ∼15-fold platelet enrichment. Several biological tests attest to the high quality of platelets obtained from the microdevice. The performance of the developed microdevice is superior to those of few available microdevices. Our work suggests that passive microdevices can attain or even surpass the performance of active microdevices.

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“…38 The filtration method by Dickson et al removed residual red blood cells from units of apheresis-derived PLTs at a flow rate of 0.1 mL min −1 . 39 A device based on deterministic lateral displacement (DLD) by Li et al purified PLTs at a rate of 1000 cells per second from whole blood diluted 50-fold. 40 A device based on the hydrodynamic lift Lab on a Chip Paper principle implemented by Geislinger et al recovered PLTs from diluted whole blood (hematocrit <1%) at a flow rate of 0.0125 mL min −1 .…”

Section: Discussionmentioning

confidence: 99%

Separation of platelets by size in a microfluidic device based on controlled incremental filtration

Dinh,

Mukhamedshin,

Abhishek

et al. 2024

Lab Chip

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A scalable, micropore, platelet rich plasma separation device

Cited by 15 publications

References 14 publications

Separation and Enrichment of Platelets from Whole Blood Using a PDMS-Based Passive Microdevice

Separation and Enrichment of Platelets from Whole Blood Using a PDMS-Based Passive Microdevice

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Separation of platelets by size in a microfluidic device based on controlled incremental filtration

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