A sandwich-type electrochemical immunosensor using polythionine/AuNPs nanocomposites as label for ultrasensitive detection of carcinoembryonic antigen

Lai, Yuxuan; Xia, Zhihuan; Li, Song; Deng, Yan; Liu, Xueying

doi:10.1166/mex.2019.1524

Cited by 33 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Te regression equation was I P (μA) � 4.6652 + 0.7570x (R 2 � 0.9981) with 0.35 pg•mL −1 detection limit. In comparison with other nanoprobe-based immunosensors [31][32][33][34][35][36][37], the detection limit of the prepared immunosensor was lower (Table 1). Te reasons for the excellent analytical performance of the designed immunosensor may be summarized as follows: frst, PANI, as the conductive shell of PB@PANI core-shell structure, maintained the structural stability of PB and efectively improved the electron transfer from PANI to PB due to the intimate adhesion.…”

Section: Quantitative Detection Of Ceamentioning

confidence: 82%

Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe

Feng

Chen

Zhang

et al. 2023

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Herein, a method was developed for the sensitive monitoring of carcinoembryonic antigen (CEA) by gold nanoparticles dotted prussian blue@polyaniline core-shell nanocubes (Au NPs/PB@PANI). First, a facile low-temperature method was used to prepare the uniform PB@PANI core-shell nanocubes with the assistance of PVP, where PB acted as the electron transfer mediator to provide electrochemical signals, and the PANI with excellent conductivity and desirable chemical stability not only played the role of a protective layer to prevent etching of PB in basic media but also effectively improved electron transfer. Importantly, to further enhance the electrical conductivity and biocompatibility of PB@PANI and to further enhance the electrochemical signal and capture a large amount of Ab2, Au NPs were doped on the surface of PB@PANI to form Au NPs/PB@PANI nanocomposites. Subsequently, benefiting from the advantages of core-shell structure nanoprobes and gold-platinum bimetallic nanoflower (AuPt NF), a sandwich-type electrochemical immunosensor for CEA detection was constructed, which provided a wide linear detection range from 1.0 pg·mL−1 to 100.0 ng·mL−1 and a low detection limit of 0.35 pg·mL−1 via DPV (at 3σ). Moreover, it displayed a satisfactory result when the core-shell structure nanoprobe-based immunosensor was applied to determine CEA in real human serum samples.

show abstract

Section: Quantitative Detection Of Ceamentioning

confidence: 82%

Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe

Feng

Chen

Zhang

et al. 2023

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

“…no. Type of sensor Sensing material Target Bio marker Lowest measured concentration References 1 ELISA – CEA, CYFRA-21 ~ng/mL 19 2 Electrochemical immunosensor Cubic CeO2 implanted reduced graphene oxide based biosensor CYFRA 21-1 0.625 pg /mL 43 3 Electrochemical immunosensor Polyhydroquinone-graphene composite CYFRA-21 2.3 pg/mL 44 4 Electrochemical immunosensor Amine-functionalized MoO3@RGO Nanohybrid-Based Biosensor CEA 0.001 ng/mL 45 5 Electrochemical immunosensor A sandwich-type electrochemical immunosensor using polythionine/AuNPs nanocomposites CEA 0.3 ng/mL 46 6 This work RGO/MEL/antibody/BSA/antigen CEA, CYFRA-21 1 pg/mLfor both the antigens …”

Section: Resultsmentioning

confidence: 99%

Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors

Joshi

Kallappa

Kumar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

One way of early diagnosis of cancer is by detecting the biomarkers that get introduced into easily accessible body fluids. We report the development of portable and rapid electronic biosensors for quantitative detection of two secretive cancer biomarkers–Carcinoembryonic antigen (CEA) and Cytokeratin fragment 19 (CYFRA 21-1). The reduced graphene oxide (rGO)/ melamine (MEL)/antibodies/ bovine serum albumin (BSA) based devices were tested for 1 pg/mL to 800 ng/mL of CEA and CYFRA 21-1. The responses of the sensors ranged from 7.14 to 59.1% and from 6.18 to 64% for 1 pg/mL to 800 ng/mL CEA and CYFRA 21-1 respectively. A read-out circuit was assembled to develop a portable prototype which was used to assess the concentrations of the two antigens present in saliva samples of 14 subjects. The prototype could accurately discriminate between 9 oral squamous cell carcinoma patients and 5 healthy controls.

show abstract

“…Among them, graphene and carbon nanotubes are probably the most commonly used materials in chemical analysis. These carbon nanomaterials can be synthesized by several methods, which can be roughly classified in top-down and bottom-up methods (Lai et al 2019 ). Their physicochemical characterization is essential to assure product quality (purity, defects, chemical species on the surface, etc.)…”

Section: Resultsmentioning

confidence: 99%

Nursing research on a new silver-based antibacterial agent for pneumonia

Wu¹,

Cao²,

Xu³

2022

Appl Nanosci

View full text Add to dashboard Cite

As a highly contagious bacterium, pneumonia can cause a series of respiratory diseases, and its treatment has become a concern of people. This study mainly discusses the nursing research of a new silver-based antibacterial agent in the treatment of pneumonia. The following procedures were performed: (1) a sterilized expectoration suction device was inserted into the nasopharynx (7–8 cm), and the nasopharyngeal secretions were suck by controlling the pressure of the suction device; and (2) the catheter was washed with 2 mL of sterilized physiological saline, and the sample was immediately sent to the laboratory for bacterial culture of the lower airway and respiratory secretions to determine viral antigens. The drug resistance coefficient of the lysozyme, benzoate, and pneumonia bacteria is 4.5–26.8%. In addition, the fourth generation sefalos posephin and pneumonia bacteria presented drug sensitivity, and the drug resistance coefficient is 13.1–33.3%. The sensitivity to the new silver-based antimicrobial agent and lopenem is 100%. The sensitivity of other germs is between 2 and 5%. The results of the study indicate that the antibacterial properties of the new silver-based antibacterial agent increase with the degree of amino acid substitution of the same sample concentration. The new silver-based antibacterial agent has excellent antibacterial properties against Klebsiella pneumoniae .

show abstract

A sandwich-type electrochemical immunosensor using polythionine/AuNPs nanocomposites as label for ultrasensitive detection of carcinoembryonic antigen

Cited by 33 publications

References 0 publications

Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe

Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe

Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors

Nursing research on a new silver-based antibacterial agent for pneumonia

Contact Info

Product

Resources

About