A sandwich SERS immunoassay platform based on a single-layer Au–Ag nanobox array substrate for simultaneous detection of SCCA and survivin in serum of patients with cervical lesions

Liu, Yifan; Ran, Menglin; Sun, Yue; Fan, Yongxin; Wang, Jinghan; Cao, Xiaowei; Lü, Dan

doi:10.1039/d1ra03082e

Cited by 10 publications

(19 citation statements)

References 49 publications

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“…Liu et al fabricated a SERSbased immunoassay to simultaneously detect squamous cell carcinoma antigen (SCCA) and survivin (Figure 13b). 261 In their unique design, the detection substrate consisted of gold− silver nanoshells (Au−Ag NSs) with DTNB and 4-amino thiophenol (4-ATP) as the Raman reporters, and monoclonal anti-SCCA and antisurvivin antibodies for targeting the respective antigen. The capture substrate consisted of a gold− silver nano box (Au-AgNB) array coated with polyclonal antibodies.…”

Section: Sers-based High Throughput Proteomic Assays For Ex Vivo Diag...mentioning

confidence: 99%

“…SERS-based immunoassays have also been developed with bimetallic NPs for detecting multiple tumor antigen markers. Liu et al fabricated a SERS-based immunoassay to simultaneously detect squamous cell carcinoma antigen (SCCA) and survivin (Figure b) . In their unique design, the detection substrate consisted of gold–silver nanoshells (Au–Ag NSs) with DTNB and 4-amino thiophenol (4-ATP) as the Raman reporters, and monoclonal anti-SCCA and antisurvivin antibodies for targeting the respective antigen.…”

Section: Labeled Surface-enhanced Raman Nanoprobes For Proteomicsmentioning

confidence: 99%

“…Detection substrate consisted of gold–silver nanoshells (Au–Ag NSs) with DTNB and 4-amino thiophenol (4-ATP) as the Raman reporters. Adapted from ref . Copyright 2021 Royal Society of Chemistry under CC BY 3.0 license ().…”

Section: Labeled Surface-enhanced Raman Nanoprobes For Proteomicsmentioning

confidence: 99%

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The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

Cutshaw,

Uthaman,

Hassan

et al. 2023

Chem. Rev.

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Omics technologies have rapidly evolved with the unprecedented potential to shape precision medicine. Novel omics approaches are imperative toallow rapid and accurate data collection and integration with clinical information and enable a new era of healthcare. In this comprehensive review, we highlight the utility of Raman spectroscopy (RS) as an emerging omics technology for clinically relevant applications using clinically significant samples and models. We discuss the use of RS both as a label-free approach for probing the intrinsic metabolites of biological materials, and as a labeled approach where signal from Raman reporters conjugated to nanoparticles (NPs) serve as an indirect measure for tracking protein biomarkers in vivo and for high throughout proteomics. We summarize the use of machine learning algorithms for processing RS data to allow accurate detection and evaluation of treatment response specifically focusing on cancer, cardiac, gastrointestinal, and neurodegenerative diseases. We also highlight the integration of RS with established omics approaches for holistic diagnostic information. Further, we elaborate on metal-free NPs that leverage the biological Raman-silent region overcoming the challenges of traditional metal NPs. We conclude the review with an outlook on future directions that will ultimately allow the adaptation of RS as a clinical approach and revolutionize precision medicine.

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Section: Sers-based High Throughput Proteomic Assays For Ex Vivo Diag...mentioning

confidence: 99%

Section: Labeled Surface-enhanced Raman Nanoprobes For Proteomicsmentioning

confidence: 99%

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The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

Cutshaw,

Uthaman,

Hassan

et al. 2023

Chem. Rev.

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“…As HPV vaccination does not protect against all high-risk HPV types [ 77 , 78 ], more work on multivariant HPV vaccines could help in this area. However, excellent quality screening programmes are still crucial in preventing cervical cancer in this new HPV vaccination era [ 79 , 80 , 81 , 82 ].…”

Section: Summary and Future Directionsmentioning

confidence: 99%

Raman Spectroscopy for Early Detection of Cervical Cancer, a Global Women’s Health Issue—A Review

Shaikh

Daniel²,

Lyng

2023

Molecules

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This review focuses on recent advances and future perspectives in the use of Raman spectroscopy for cervical cancer, a global women’s health issue. Cervical cancer is the fourth most common women’s cancer in the world, and unfortunately mainly affects younger women. However, when detected at the early precancer stage, it is highly treatable. High-quality cervical screening programmes and the introduction of the human papillomavirus (HPV) vaccine are reducing the incidence of cervical cancer in many countries, but screening is still essential for all women. Current gold standard methods include HPV testing and cytology for screening, followed by colposcopy and histopathology for diagnosis. However, these methods are limited in terms of sensitivity/specificity, cost, and time. New methods are required to aid clinicians in the early detection of cervical precancer. Over the past 20 years, the potential of Raman spectroscopy together with multivariate statistical analysis has been shown for the detection of cervical cancer. This review discusses the research to date on Raman spectroscopic approaches for cervical cancer using exfoliated cells, biofluid samples, and tissue ex vivo and in vivo.

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“…Several studies have shown the potential of this technique for cervical precancer and cancer diagnosis based on changes to the biochemistry of cervical tissues and cells [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. In addition, both Raman spectroscopy [ 18 , 19 , 20 ] and surface-enhanced Raman spectroscopy (SERS) using gold or silver nanoparticles [ 21 , 22 , 23 , 24 ] have shown good potential for cervical cancer detection using blood serum or plasma. Apart from studies on pellets of exfoliated cells [ 25 , 26 ] and our own studies on single exfoliated cells [ 13 , 15 , 17 , 27 , 28 , 29 ], there have been relatively few cytology studies using Raman spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Raman Spectroscopic Classification Model for Cervical Intraepithelial Neoplasia (CIN)

Traynor

Duraipandian

Bhatia

et al. 2022

Cancers

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The mortality associated with cervical cancer can be reduced if detected at the precancer stage, but current methods are limited in terms of subjectivity, cost and time. Optical spectroscopic methods such as Raman spectroscopy can provide a rapid, label-free and nondestructive measurement of the biochemical fingerprint of a cell, tissue or biofluid. Previous studies have shown the potential of Raman spectroscopy for cervical cancer diagnosis, but most were pilot studies with small sample sizes. The aim of this study is to show the clinical utility of Raman spectroscopy for identifying cervical precancer in a large sample set with validation in an independent test set. Liquid-based cervical cytology samples (n = 662) (326 negative, 200 cervical intraepithelial neoplasia (CIN)1 and 136 CIN2+) were obtained as a training set. Raman spectra were recorded from single-cell nuclei and subjected to a partial least squares discriminant analysis (PLSDA). In addition, the PLSDA classification model was validated using a blinded independent test set (n = 69). A classification accuracy of 91.3% was achieved with only six of the blinded samples misclassified. This study showed the potential clinical utility of Raman spectroscopy with a good classification of negative, CIN1 and CIN2+ achieved in an independent test set.

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A sandwich SERS immunoassay platform based on a single-layer Au–Ag nanobox array substrate for simultaneous detection of SCCA and survivin in serum of patients with cervical lesions

Abstract: A novel SERS immunoassay platform for simultaneous detection of SCCA and survivin based on Au–Ag nanoshells (Au–AgNSs) combined with a single-layer Au–AgNB array substrate was proposed in the current study.

Cited by 10 publications

References 49 publications

The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine

Raman Spectroscopy for Early Detection of Cervical Cancer, a Global Women’s Health Issue—A Review

Development and Validation of a Raman Spectroscopic Classification Model for Cervical Intraepithelial Neoplasia (CIN)

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