“…Therefore, a rapid detection method should be applied for on-site COVID-19 identification in the environment. Loop-mediated isothermal amplification (LAMP) has achieved brilliant performance in pathogenic virus detection, accomplishing amplification within 45 min ( Liu et al, 2018 ). LAMP assay also performs nucleic acid amplification without requiring nucleic acid extraction ( Lalli et al, 2020 ), thus preventing RNA damage through a tedious process.…”
SARS-Cov-2 has erupted across the globe, and confirmed cases of COVID-19 pose a high infection risk. Infected patients typically receive their treatment in specific isolation wards, where they are confined for at least 14 days. The virus may contaminate any surface of the room, especially frequently touched surfaces. Therefore, surface contamination in wards should be monitored for disease control and hygiene purposes. Herein, surface contamination in the ward was detected on-site using an RNA extraction-free rapid method. The whole detection process, from surface sample collection to readout of the detection results, was finished within 45 min. The nucleic acid extraction-free method requires minimal labor. More importantly, the tests were performed on-site and the results were obtained almost in real-time. The test confirmed that 31 patients contaminated seven individual sites. Among the sampled surfaces, the electrocardiogram fingertip presented a 72.7% positive rate, indicating that this surface is an important hygiene site. Meanwhile, the bedrails showed the highest correlation with other surfaces, so should be detected daily. Another surface with high contamination risk was the door handle in the bathroom. To our knowledge, we present the first on-site analysis of COVID-19 surface contamination in wards. The results and applied technique provide a potential further reference for disease control and hygiene suggestions.
“…Therefore, a rapid detection method should be applied for on-site COVID-19 identification in the environment. Loop-mediated isothermal amplification (LAMP) has achieved brilliant performance in pathogenic virus detection, accomplishing amplification within 45 min ( Liu et al, 2018 ). LAMP assay also performs nucleic acid amplification without requiring nucleic acid extraction ( Lalli et al, 2020 ), thus preventing RNA damage through a tedious process.…”
SARS-Cov-2 has erupted across the globe, and confirmed cases of COVID-19 pose a high infection risk. Infected patients typically receive their treatment in specific isolation wards, where they are confined for at least 14 days. The virus may contaminate any surface of the room, especially frequently touched surfaces. Therefore, surface contamination in wards should be monitored for disease control and hygiene purposes. Herein, surface contamination in the ward was detected on-site using an RNA extraction-free rapid method. The whole detection process, from surface sample collection to readout of the detection results, was finished within 45 min. The nucleic acid extraction-free method requires minimal labor. More importantly, the tests were performed on-site and the results were obtained almost in real-time. The test confirmed that 31 patients contaminated seven individual sites. Among the sampled surfaces, the electrocardiogram fingertip presented a 72.7% positive rate, indicating that this surface is an important hygiene site. Meanwhile, the bedrails showed the highest correlation with other surfaces, so should be detected daily. Another surface with high contamination risk was the door handle in the bathroom. To our knowledge, we present the first on-site analysis of COVID-19 surface contamination in wards. The results and applied technique provide a potential further reference for disease control and hygiene suggestions.
One of the major challenges for scientists and engineers today is to develop technologies for the improvement of human health in both developed and developing countries. However, the need for cost-effective, high-performance diagnostic techniques is very crucial for providing accessible, affordable, and high-quality healthcare devices. In this context, microfluidic-based devices (MFDs) offer powerful platforms for automation and integration of complex tasks onto a single chip. The distinct advantage of MFDs lies in precise control of the sample quantities and flow rate of samples and reagents that enable quantification and detection of analytes with high resolution and sensitivity. With these excellent properties, microfluidics (MFs) have been used for various applications in healthcare, along with other biological and medical areas. This review focuses on the emerging demands of MFs in different fields such as biomedical diagnostics, environmental analysis, food and agriculture research, etc., in the last three or so years. It also aims to reveal new opportunities in these areas and future prospects of commercial MFDs.
“…With the help of MembR valves, all the steps from sample lysis, RNA extraction and purification, to amplification using real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) and RNA detection were integrated on the single device and applied to the detection of avian influenza viruses (HPAIVs). The whole set-up, controlled by a laptop, included accurate temperature control and weighed just 4 kg, in agreement with the requirements of a POC platform ( Figure 7 ) [ 84 ].…”
Section: Poc Tools For Personalized Medicinementioning
confidence: 99%
“…( C ) Design of the diagnostic platform including six reservoirs with different solutions, a fibre-packed channel, an aliquoting structure, and six chambers for RNA extraction and RT-LAMP reaction; six different MembR valves, four transfer chambers and waste chambers. Reproduced with permission from [ 84 ].…”
Section: Figurementioning
confidence: 99%
“…Second, the sample adding area of the paper was cut and put in a micro-well for subsequent isothermal amplification. The nucleic acid captured on the glass fibre of the paper was directly used as the template for the high-efficiency LAMP reaction, and the results were visible by the naked eye on the basis of color (rose red positive for or brown for negative) ( B ) Modified with permission from [ 84 ].…”
A major trend in biomedical engineering is the development of reliable, self-contained point-of-care (POC) devices for diagnostics and in-field assays. The new generation of such platforms increasingly addresses the clinical and environmental needs. Moreover, they are becoming more and more integrated with everyday objects, such as smartphones, and their spread among unskilled common people, has the power to improve the quality of life, both in the developed world and in low-resource settings. The future success of these tools will depend on the integration of the relevant key enabling technologies on an industrial scale (microfluidics with microelectronics, highly sensitive detection methods and low-cost materials for easy-to-use tools). Here, recent advances and perspectives will be reviewed across the large spectrum of their applications.
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