A sample pretreatment method to suit the metabolomic analysis of Bacillus licheniformis based on GC-MS

Wang, H. B.; Feng, Yongrui; Gui, Shuqi; Zhang, Y.; Lu, Fuping

doi:10.1039/c7ay00008a

Cited by 6 publications

(7 citation statements)

References 38 publications

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“…Quenching with cold methanol: water (8 : 2, −20°C) successfully retrieved abundance of polar metabolites, especially coenzyme A (CoA) and CoA thioester derivatives, citric acid, and some nucleotides in Caulobacter crescentus [ 59 ]. The use of 60% methanol (−40°C)/0.9% ammonium carbonate (NH 4 HCO 3 ) detected about 127 metabolites of Bacillus licheniformis , where the salt helped in improving the exudation of metabolites as it reduces the osmotic stress on the cells [ 87 ].…”

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

“…The choice of extraction solvent depends on a number of factors including the total sample volume, the extraction time, and the coverage of metabolites [ 96 ]. The mechanical methods include the use of ultrasonic bath [ 83 ], glass bead beating [ 49 , 52 , 72 , 82 ], bead mill [ 53 , 87 ], freeze-thaw [ 55 , 56 , 59 , 71 , 84 , 97 ], and the least favourable, supercritical fluid extraction [ 98 ]. Mechanical extraction is commonly applied for animal and plant samples but it is least preferred for microbial intracellular metabolites as the method releases both small and large metabolites [ 30 ].…”

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

“…Mechanical extraction is commonly applied for animal and plant samples but it is least preferred for microbial intracellular metabolites as the method releases both small and large metabolites [ 30 ]. To enhance the efficiency of metabolite extraction, a number of studies have been reported on the combination of chemical and mechanical methods including methanol/ethanol with freeze-thaw cycles [ 55 , 56 , 59 , 66 , 71 , 84 , 97 ], methanol/ethanol with bead mill [ 53 ], bead milling in liquid nitrogen [ 87 ], and glass bead beating [ 49 ], as well as sonication with methanol [ 55 ] and ethanol [ 83 ].…”

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

“…Similarly, bead milling in liquid nitrogen showed better identification of B . licheniformis metabolites than ultrasonic bath as bead milling operated less manually with greater cell disruption rate [ 87 ]. Meanwhile, extremely low temperature conditions due to liquid nitrogen maintain the stability of metabolites during the cell disruption process [ 87 ].…”

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

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Bacterial Metabolomics: Sample Preparation Methods

Kamal

Maifiah

Rahim

et al. 2022

Biochemistry Research International

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Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of the important “omics” tools, the approach has been widely employed in various fields in helping to better understand the complex cellular metabolic states and changes. Bacterial metabolomics has gained a significant interest as bacteria serve to provide a better subject or model at systems level. The approach in metabolomics is categorized into untargeted and targeted which serves different paradigms of interest. Nevertheless, the bottleneck in metabolomics has been the sample or metabolite preparation method. A custom-made method and design for a particular species or strain of bacteria might be necessary as most studies generally refer to other bacteria or even yeast and fungi that may lead to unreliable analysis. The paramount aspect of metabolomics design comprises sample harvesting, quenching, and metabolite extraction procedures. Depending on the type of samples and research objective, each step must be at optimal conditions which are significantly important in determining the final output. To date, there are no standardized nor single designated protocols that have been established for a specific bacteria strain for untargeted and targeted approaches. In this paper, the existing and current developments of sample preparation methods of bacterial metabolomics used in both approaches are reviewed. The review also highlights previous literature of optimized conditions used to propose the most ideal methods for metabolite preparation, particularly for bacterial cells. Advantages and limitations of methods are discussed for future improvement of bacterial metabolomics.

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Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

Section: Sample Preparation Methods For Bacterial Metabolitesmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial Metabolomics: Sample Preparation Methods

Kamal

Maifiah

Rahim

et al. 2022

Biochemistry Research International

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“…erefore, the original taste, flavor, color, and nutrients of the sample were well maintained. According to some reports, 127 metabolites were detected in the metabonomic analysis of Bacillus licheniformis, including a large number of carbohydrates, amino acids, and organic acids, which produced furfural or benzaldehyde as reactants in the Maillard reaction [32].…”

Section: Fingerprints Of Ymp With Different Sterilization Methodsmentioning

confidence: 99%

Analysis of Volatile Organic Compounds by HS-GC-IMS in Powdered Yak Milk Processed under Different Sterilization Conditions

Feng

Wang

et al. 2021

Journal of Food Quality

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Headspace-gas chromatography-ion mobility spectroscopy (HS-GC-IMS) was used to detect the volatile organic compounds (VOCs) of yak milk powders (YMPs) under ultra-high-pressure sterilization (UHPS) and thermization (TH) methods. The analyses led to the identification of several characteristic of compounds, therefore, exploitation and comparison of the different flavors. A total of 46 peaks were detected, and 17 compounds were identified, including 7 aldehydes, 5 ketones, 3 acids, 1 terpene, and 1 ester. Furthermore, principal component analysis (PCA) and fingerprint similarity analysis based on Euclidean distance compared the YMPs and found that the YMPs had certain differences, which can distinguish the YMPs with different sterilization methods. In conclusion, different sterilization methods possibly affect the flavor of YMPs, and UHPS is bettedslfr than TH. Also, aldehydes were mainly be detected in UHPS groups, whereas the ketones and acids mostly appeared in TH groups. Most importantly, UHPS can retain the original flavor of yak milk to a greater extent.

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