A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection

Diuzheva, Alina; Balogh, József; Studenyak, Yaroslav; Cziáky, Zoltán; Jekő, József

doi:10.1016/j.talanta.2018.10.026

Cited by 23 publications

(9 citation statements)

References 28 publications

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“…Most of the methods had the problems associated with large volumes or time consumption. Even a liquid-liquid microextraction required 600 μL of organic solvent, and only 100 μL extract were obtained as a result [16]. In our project, an organic solvent protein precipitation extractive agent was selected to facilitate a shorter extraction time and low amount of organic solvent.…”

Section: Optimization Of Uplc-ms/ms Conditionsmentioning

confidence: 99%

Validated UPLC-MS/MS method for the determination of ivosidenib in rat plasma: Application to a pharmacokinetic study

Chen¹,

Chen

et al. 2023

AChrom

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Ivosidenib (AG-120) is an unlisted, but estimated to be valid, oral inhibitor for isocitrate dehydrogenase 1 (IDH1) in the phase Ⅰ study of IDH1-mutated acute myeloid leukemia (AML) patients. This paper presents the investigation and validation of a rapid, effective, qualitative and quantitative determination method of ivosidenib in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The samples were treated using acetonitrile precipitation to remove protein influence. Then, the supernatant was extracted to analyze plasma concentration traits. In the UPLC system, acetonitrile and water containing 0.1% formic acid were selected as a cosolvent mobile phase, applying a gradient elution to isolate compounds in a C18 column. Mass detections were performed on a triple quadruple mass spectrometer in positive ion mode. Electroshock characteristic fragment ionization was used for m/z 583.95→214.53 for ivosidenib for quantitative determination, m/z 583.95→186.6 for qualitative determination, and m/z 492.06→354.55 for IS. The selectivity, linearity, stability, accuracy and precision were verified by reaching the guideline criteria from European Medicine Agency (EMA) and the Food and Drug Administration (FDA). The calibration curve was linear over the concentration range of 2–2,000 ng mL−1 for ivosidenib in rat plasma with a lower limit of quantification (LLOQ) of at least 2 ng mL−1. Additionally, there was no distinct matrix effect or carry-over phenomenon. The method was successfully established and applied to separate ivosidenib from plasma, with the entire analytical process being performed within 3 min for each sample, which shows high-efficiency and convenience for further studies of ivosidenib.

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Section: Optimization Of Uplc-ms/ms Conditionsmentioning

confidence: 99%

Validated UPLC-MS/MS method for the determination of ivosidenib in rat plasma: Application to a pharmacokinetic study

Chen¹,

Chen

et al. 2023

AChrom

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“…The HLLE in this case should start with forming a single homogenous phase, then adding a definite amount of EPPS until complete phase separation was achieved, and EPPS was precipitated. As shown in Table 2, SALLE has been extensively used in drugs [71][72][73], toxicants [74], pesticides [75] and active ingredients [76] in various matrices including biological fluids [77,78], food products [19,79], and water [80].…”

Section: F I G U R E 1 Procedures Of Salting-out Induced Homogeneous Liquid-liquid Extractionmentioning

confidence: 99%

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Hammad

Abdallah

Bedair

et al. 2021

J of Separation Science

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Liquid-liquid extraction is a widely used technique of sample preparation in biomedical analysis. In spite of the high pre-concentration capacities of liquidliquid extraction, it suffers from a number of limitations including time and effort consumption, large organic solvent utilization, and poor performance in highly polar analytes. Homogeneous liquid-liquid extraction is an alternative sample preparation technique that overcomes some drawbacks of conventional liquid-liquid extraction, and allows employing greener organic solvents in sample treatment. In homogeneous liquid-liquid extraction, a homogeneous phase is formed between the aqueous sample and the water-miscible extractant, followed by chemically or physically induced phase separation. To form the homogeneous phase, aqueous samples are mixed with water-miscible organic solvents, waterimmiscible solvents/cosolvents, surfactants, or smart polymers. Then, phase separation is induced chemically (adding salt, sugar, or buffer) or physically (changing temperature or pH). This mode is rapid, sustainable, and cost-effective in comparison with other sample preparation techniques. Moreover, homogeneous liquid-liquid extraction is more suitable for the extraction of delicate macromolecules such as enzymes, hormones, and proteins and it is more compatible with liquid chromatography with tandem mass spectrometry, which is a vital technique in metabolomics and proteomics. In this review, the principle, types, applications, automation, and technical aspects of homogeneous liquid-liquid extraction are discussed.

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“…L-Cysteine (Cys) is a non-essential amino acid with a thiol group (Sahu et al 2021), which plays a critical role in different biological processes, such as protein synthesis (Remond et In comparison, spectrophotometric methods are simple, fast, and low-cost, which provides a convenient and e cient method for assaying substances. A number of spectrophotometric methods have been reported for the determination of Cys (Diuzheva et al 2019;Gaitonde 1967;Tütem and Apak 1991). Gaitonde (Gaitonde 1967) reported a spectrophotometric determination of Cys by copper(II)-neocuproin reagent requiring heat.…”

Section: Introductionmentioning

confidence: 99%

“…Gaitonde (Gaitonde 1967) reported a spectrophotometric determination of Cys by copper(II)-neocuproin reagent requiring heat. Diuzheva (Diuzheva et al 2019) developed a salting-out assisted liquid-liquid extraction procedure for the determination of Cys, based on its interaction with phenazine methasulfate. The method could be applied in food chemical analysis, but requires the use of toxic acetonitrile.…”

Section: Introductionmentioning

confidence: 99%

Two spectrophotometric methods for the determination of cysteine in dietary supplements

Liu¹,

Yuan²,

Chu³

et al. 2022

Preprint

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Two rapid, sensitive, extraction-free spectrophotometric methods were developed for the determination of cysteine. The procedures were based on the addition reaction of cysteine with Aniline blue water soluble (Method A) or cysteine with Acid Fuchsin (Method B) in borax-sodium hydroxide medium, which formed a colorless thioether derivative, and resulting in a decrease in absorbance at wavelengths of 584 and 540 nm respectively. The cysteine complied with Beer's Law in the concentration range of 0.20~2.40 mg/L and 0.50~6.00 mg/L with good precision and accuracy, whose limits of detection were 0.122 mg/L at 584 nm for Method A and 0.113 mg/L at 540 nm for Method B, respectively. The proposed methods have been successfully applied to the determination of cysteine in dietary supplements. The analytical results of the actual samples were in accordance with those by the copper(II)-neocuproin reagent spectrophotometric method.

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A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection

Cited by 23 publications

References 28 publications

Validated UPLC-MS/MS method for the determination of ivosidenib in rat plasma: Application to a pharmacokinetic study

Validated UPLC-MS/MS method for the determination of ivosidenib in rat plasma: Application to a pharmacokinetic study

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Two spectrophotometric methods for the determination of cysteine in dietary supplements

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