A salt tolerant Enterobacter cloacae mutant for bioaugmentation of petroleum- and salt-contaminated soil

Hua, Xueming; Wang, Jun; Wu, Zuojun; Zhang, Hongxing; Li, Heping; Xing, Xinghui; Liu, Zheng

doi:10.1016/j.bej.2009.12.014

Cited by 85 publications

(30 citation statements)

References 28 publications

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“…for strain-D, strain-S, strain-O, and strain-I, respectively. Bacteria of the genera Pseudomonas, Enterobacter, and Bacillus have been widely reported as bacteria that can degrade the organic compounds in petroleum oil and gas wastes, including oilfieldproduced water [9,20], soil contaminated with hydrocarbons [21][22][23][24], and wastewater contaminated with hydrocarbons [10]. A recent study reported that the genus Enterobacter also has the ability to degrade the biopolymers that are used in oil-recovery processes [25].…”

Section: Bioreactor Design/operation and Determination Of The Biokinementioning

confidence: 99%

Biological Treatment of Synthetic Oilfield-Produced Water in Activated Sludge Using a Consortium of Endogenous Bacteria Isolated from A Tropical Area

Kardena¹,

Hidayat²,

Nora³

et al. 2017

J Pet Environ Biotechnol

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The aim of this study was to investigate the biological treatment of synthetic oilfield-produced water in activated sludge in an attempt to remove the organic compounds using endogenous bacteria; we also hope to determine the biokinetic coefficients. The activated sludge was operated with various hydraulic retention times (HRT=20 hours, 12 hours, 8 hours), solid retention times (SRT=25 days, 20 days, 15 days, 10 days), and substrate concentrations (500 mg L -1 to 1,100 mg L -1 ). The endogenous bacterial strains, which were isolated from existing wastewater treatment facilities, were identified as Pseudomonas sp., Enterobacter sp., Bacillus sp1., and Bacillus sp2. It was observed that the highest COD removals were obtained in reactors A (80.7%) and B (82.4%), which had high SRTs (25 days and 20 days) and HRT (20 hours). At shorter SRTs (15 days and 20 days), the concentration of the COD effluent did not comply with the Indonesian regulations for oilfield-produced water quality standards, which means that these SRTs were not recommended as appropriate operational conditions. Furthermore, the results showed that the yield (Y), decay coefficient (kd), maximum specific growth rate (k), and saturation constant (K s ) were 0.533 mg MLVSS mg -1 COD, 0.167 day -1 , 0.985 day -1 , and 255.46 mg COD L -1 , respectively. These biokinetic coefficients (obtained from the Y and K s values) indicated that although the strains of bacteria can grow well in the reactor, they had low affinities to the substrate, which caused the concentration of the COD effluent to be relatively high.

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Section: Bioreactor Design/operation and Determination Of The Biokinementioning

confidence: 99%

Biological Treatment of Synthetic Oilfield-Produced Water in Activated Sludge Using a Consortium of Endogenous Bacteria Isolated from A Tropical Area

Kardena¹,

Hidayat²,

Nora³

et al. 2017

J Pet Environ Biotechnol

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“…To evaluate salt tolerance, BF40 strain was grown in Luria-Bertani (LB) medium with NaCl additions, i.e., 0, 2.0, 4.0, 6.0, and 8.0% at 30°C on a rotary shaker at 150 rpm (Hua et al 2010). Growth was monitored through spectrophotometry at optical density 630 (OD 630 ) at 2, 3, 4, 5, 6, 8, 10, 12, 14, 18, 22, 26, and 32 h. Surface tension and emulsification activity were employed to evaluate the surface-active properties using culture supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…Due to pipeline leakage, mismanagement, and offshore oil production, petroleum-and salt-contaminated soils are often found in oilfields and coastal zones (Pezeshki et al 2000, Nicholson andFathepure 2004). Salinity is a key factor which can inhibit microbial growth and subsequent degradation of petroleum contaminants (Hua et al 2010), and high salt concentrations (400 mmol NaCl) can inhibit microbial growth in soils by more than 90% (Rousk et al 2011). Rhykerd et al (1995) found that salt concentrations of 200 dS/m can decrease oil mineralization in soils by 20-44%.…”

mentioning

confidence: 99%

Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

Wu¹,

Xie²,

Yi³

et al. 2012

PLANT SOIL ENVIRON

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An ideal strain for crude oil degradation in saline soils would be one with high salt-tolerance. A novel bacterial strain, Serratia sp. BF40, was isolated from crude oil contaminated saline soils. Its salt-tolerance, surface activity and ability to degrade crude oil in saline soils were evaluated. It can grow in liquid culture with NaCl concentration less than 6.0%. Its surface activity characterized as an efficient surface tension reduction, was significantly affected by salinity above 2.0%. BF40 inoculation could decrease surface tension of soil solutions and facilitate crude oil removal in soils with 0.22-1.20% salinity, but the efficiency was both significantly lower than its biosurfactant addition. The BF40 strain has a high potential for biodegradation of crude oil contaminated saline soils in view of its high surface activity and salt-tolerance, which is the first report of biosurfactant producing by the genus Serratia for petroleum degrading. We suggest that biosurfactant addition is an efficient strategy. Simultaneously, the growing status of the strain and how to boost its surface activity in saline soils should deserve further studies in order to achieve a continuous biosurfactant supply.

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“…It has been reported that ARTP has a higher positive genotoxic response than traditional mutation methods, making it a potentially effective mutation breeding strategy (Hua et al, 2010;Wang et al, 2010;Li et al, 2014;Zhang et al, 2014). In this study, ARTP-irradiation was used for inducing random mutations, and high AD-producing M. neoaurum mutants were selected later.…”

Section: Mutagenesis and Mutant Selectionmentioning

confidence: 99%

“…Among the different types of atmospheric pressure non-equilibrium discharge (APNED) plasma sources, atmospheric and room temperature plasma (ARPT), which is driven by a radio frequency (RF) power supply with water-cooled, bare-metallic electrodes, has shown promise in applications in biotechnology . In this context, the use of a novel mutation method such as ARTP has become increasingly popular due to its efficiency, safety, and environment-friendly nature (Hua et al, 2010;Wang et al, 2010;Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment

Liu

Zhang

Rao

et al. 2015

J. Zhejiang Univ. Sci. B

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Steroid medication is used extensively in clinical applications and comprises a large and vital part of the pharmaceutical industry. However, the difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) restricts the application of the microbial transformation of phytosterols in the industry. A novel atmospheric and room temperature plasma (ARTP) treatment, which employs helium as the working gas, was used to generate Mycobacterium neoaurum mutants producing large amounts of AD. After treatment of cultures with ARTP, four mutants were selected using a novel screening method with a color assay. Among the mutants, M. neoaurum ZADF-4 was considered the best candidate for industrial application. When the fermentation medium contained 15 g/L phytosterols and was cultivated on a rotary shaker at 160 r/min at 30 °C for 7 d, (6.28±0.11) g/L of AD and (0.82±0.05) g/L of ADD were produced by the ZADF-4 mutant, compared with (4.83±0.13) g/L of AD and (2.34±0.06) g/L of ADD by the original strain, M. neoaurum ZAD. Compared with ZAD, the molar yield of AD increased from 48.3% to 60.3% in the ZADF-4 mutant. This result indicates that ZADF-4 may have potential for industrial production of AD.

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A salt tolerant Enterobacter cloacae mutant for bioaugmentation of petroleum- and salt-contaminated soil

Cited by 85 publications

References 28 publications

Biological Treatment of Synthetic Oilfield-Produced Water in Activated Sludge Using a Consortium of Endogenous Bacteria Isolated from A Tropical Area

Biological Treatment of Synthetic Oilfield-Produced Water in Activated Sludge Using a Consortium of Endogenous Bacteria Isolated from A Tropical Area

Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment

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