A salt stress-activated mitogen-activated protein kinase in soybean is regulated by phosphatidic acid in early stages of the stress response

Im, Jong Hee; Lee, Hyoungseok; Kim, Ji-Tae; Kim, Ho Bang; Kim, Se-Young; Kim, B. Moon; An, Chung Sun

doi:10.1007/s12374-011-0036-8

Cited by 14 publications

(15 citation statements)

References 35 publications

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“…In our previous study, GMK1 is activated by PA (Im et al, 2012). Therefore, it is reasonable to speculate that GMK1 activation is regulated by both PA and H 2 O 2 .…”

Section: Resultsmentioning

confidence: 82%

“…Reactive oxygen species were generated in salt-treated soybean roots Our previous study suggested that the PA signaling controls GMK1 activity up to 5 min of NaCl treatment (Im et al, 2012). However, salt-inducible GMK1 activity was persistent up to 30 min.…”

Section: Resultsmentioning

confidence: 94%

“…Activity of GMK1 is regulated by PA and hydrogen peroxide at different time points in salt stress Previous studies indicate that both PA and ROS activate GMK1. In salt stress, PA reached its maximum level at 5 min (Im et al, 2012) but H 2 O 2 was significantly increased even after 30 min of NaCl treatment (Fig. 1B).…”

Section: Dmentioning

confidence: 93%

“…In our previous study, we found that soybean MAPK, GMK1 activity was strongly induced within 5 min of 300 mM NaCl treatment; this early activity is regulated by PA (Im et al, 2012). In this study, we examined regulation of GMK1 at late time periods of the treatment, because we have already shown that GMK1 activity is retained for up to 30 min.…”

Section: Introductionmentioning

confidence: 92%

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Soybean MAPK, GMK1 Is Dually Regulated by Phosphatidic Acid and Hydrogen Peroxide and Translocated to Nucleus during Salt Stress

Lee

Kim

et al. 2012

Molecules and Cells

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Mitogen-activated protein kinase (MAPK) is activated by various biotic and abiotic stresses. Salt stress induces two well-characterized MAPK activating signaling molecules, phosphatidic acid (PA) via phospholipase D and phospholipase C, and reactive oxygen species (ROS) via nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase. In our previous study, the activity of soybean MAPK, GMK1 was strongly induced within 5 min of 300 mM NaCl treatment and this early activity was regulated by PA. In this study, we focused on the regulation of GMK1 at the later stage of the salt stress, because its activity was strongly persistent for up to 30 min. H(2)O(2) activated GMK1 even in the presence of PA generation inhibitors, but GMK1 activity was greatly decreased in the presence of diphenyleneiodonium, an inhibitor of NADPH-oxidase after 5 min of the treatment. On the contrary, the n-butanol and neomycin reduced GMK1 activity within 5 min of the treatment. Thus, GMK1 activity may be sustained by H(2)O(2) 10 min after the treatment. Further, GMK1 was translocated into the nucleus 60 min after NaCl treatment. In the relationship between GMK1 and ROS generation, ROS generation was reduced by SB202190, a MAPK inhibitor, but was increased in protoplast overexpressing TESD-GMKK1. However, these effects were occurred at prolonged time of NaCl treatment. These data suggest that GMK1 indirectly regulates ROS generation. Taken together, we propose that soybean GMK1 is dually regulated by PA and H(2)O(2) at a time dependant manner and translocated to the nucleus by the salt stress signal.

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“…In our previous study, GMK1 is activated by PA (Im et al, 2012). Therefore, it is reasonable to speculate that GMK1 activation is regulated by both PA and H 2 O 2 .…”

Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 94%

Section: Dmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 92%

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Soybean MAPK, GMK1 Is Dually Regulated by Phosphatidic Acid and Hydrogen Peroxide and Translocated to Nucleus during Salt Stress

Lee

Kim

et al. 2012

Molecules and Cells

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“…Exogenous application of neomycine as PLC inhibitor showed non-significant effect on shoot fresh and dry weights while shoot length of all rice cultivars (Bas-385, Bas-2000, Bas-370 and Shaheen) was slightly increased. It has been reported that salt stress inhibits plant growth and development by activating kinase-dependent pathways and increased with increasing concentration of salt stress concentration however, neomycin a phospholipase C inhibitor inhibits this activity by increasing growth characteristics in soybean (Im et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Morpho-physiological Modulations in Rice (Oryza sativa) by Foliar Application of Phospholipase-C Inhibitor Neomycine under Saline Conditions

Masood¹,

Shahbaz²

2016

IJAB

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The present study was conducted to assess modulation in morpho-physiological attributes of rice (Oryza sativa L.) cultivars (Bas-385, Bas-2000, Bas-370 and Shaheen) at vegetative stage by phospholipase C (PLC) inhibitor neomycine (control and 100 µM) under salt stress (control, 50, 100 and 150 mM NaCl). Salt stress significantly reduced shoot fresh and dry weights, shoot length, water potential, osmotic potential, net CO 2 assimilation rate (A), transpiration rate (E), stomatal conductance (g s ), non-photochemical quenching (NPQ), coefficient of non-photochemical quenching (q N ) and photochemical quenching (q P ) while increased efficiency of photosystem-II (Fv/Fm), leaf free proline, shoot and root Na . Of all rice cultivars, cv. Bas-2000 performed better in growth, water relation and photosynthetic attributes under saline and non-saline conditions.

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Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5

Shi

Zhang

et al. 2021

Mol Biol Rep

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The mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively. In the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR assay revealed that the expression of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C ), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl2), which suggested that the RmMAPK might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.

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A salt stress-activated mitogen-activated protein kinase in soybean is regulated by phosphatidic acid in early stages of the stress response

Cited by 14 publications

References 35 publications

Soybean MAPK, GMK1 Is Dually Regulated by Phosphatidic Acid and Hydrogen Peroxide and Translocated to Nucleus during Salt Stress

Soybean MAPK, GMK1 Is Dually Regulated by Phosphatidic Acid and Hydrogen Peroxide and Translocated to Nucleus during Salt Stress

Morpho-physiological Modulations in Rice (Oryza sativa) by Foliar Application of Phospholipase-C Inhibitor Neomycine under Saline Conditions

Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5

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