A salicylic acid inducible mulberry WRKY transcription factor, MiWRKY53 is involved in plant defence response

Negi, Nisha; Khurana, Paramjit

doi:10.1007/s00299-021-02710-8

Cited by 18 publications

(9 citation statements)

References 69 publications

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“…In addition, the promoter region of LcWRKY17 contained one TCA element and one TC-rich repeat motif. Previous studies confirmed that TCA elements are important cis-acting elements involved in SA responsiveness (Negi & Khurana, 2021). SA is a well-studied phenolic plant hormone that plays an important role in plant defense.…”

Section: Discussionmentioning

confidence: 84%

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

Liu,

Jiang,

Wen

et al. 2023

Agronomy Journal

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WRKY proteins comprise a large family of transcription factors that play important roles in plant growth, development, and responses to stress, but little information is available about these genes in lychee (Litchi chinensis Sonn.). Despite being a popular fruit worldwide, lychee is highly vulnerable to lychee downy blight (LDB), a major disease affecting lychee. Based on lychee whole‐genome data, a total of 50 LcWRKY genes were identified across 15 chromosomes. According to a conserved domain and phylogenetic analysis, these 50 LcWRKYs were classified into three distinct subfamilies. Gene structure analysis revealed the presence of 2‐11 exons in the LcWRKYs. Cis‐acting regulatory element analysis identified many stress‐response elements in LcWRKY promoters. Moreover, a comprehensive two‐year investigation involving the screening of a lychee natural population led to the identification of Guiwei (GW) and Yurong 1 (YR1) as displaying stable susceptibility and resistance against LDB, respectively. Furthermore, expression patterns indicated that LcWRKY13, LcWRKY17, and LcWRKY34 were significantly upregulated in response to LDB induction, while LDB suppressed the expression of LcWRKY11. These findings suggest that these four genes may play a crucial role in lychee resistance to LDB. The present study has provided a foundation for future investigations of LcWRKY gene function in lychee.This article is protected by copyright. All rights reserved

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Section: Discussionmentioning

confidence: 84%

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

Liu,

Jiang,

Wen

et al. 2023

Agronomy Journal

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“…To build a suite of constitutive promoters with a wide range of transgene expression strengths as a plant synthetic biology toolkit, we selected 15 genes spanning different mean abundance of transcripts from the enriched candidate pool (Table ). We extracted a region of interest starting two kilobases upstream of the start codon to the beginning of the coding sequence of each gene to ensure capture of cis -regulatory elements responsible for their respective transcriptional activity. − Promoters were inserted into a pCAMBIA backbone upstream of PhytoBrick compatible GFP dropout cassette for facile construction of plasmid designs for metabolic engineering or plant synthetic biology (Figure A). This simple cloning strategy allows for the GFP dropout cassette to be replaced by the transgene of interest; thus, bacterial colonies with successful construction of the plasmid that have lost the GFP dropout cassette no longer fluoresce green and can be easily screened for on an agar plate.…”

Section: Resultsmentioning

confidence: 99%

A Suite of Constitutive Promoters for Tuning Gene Expression in Plants

et al. 2023

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The need for convenient tools to express transgenes over a large dynamic range is pervasive throughout plant synthetic biology; however, current efforts are largely limited by the heavy reliance on a small set of strong promoters, precluding more nuanced and refined engineering endeavors in planta. To address this technical gap, we characterize a suite of constitutive promoters that span a wide range of transcriptional levels and develop a GoldenGate-based plasmid toolkit named PCONS, optimized for versatile cloning and rapid testing of transgene expression at varying strengths. We demonstrate how easy access to a stepwise gradient of expression levels can be used for optimizing synthetic transcriptional systems and the production of small molecules in planta. We also systematically investigate the potential of using PCONS as an internal standard in plant biology experimental design, establishing the best practices for signal normalization in experiments. Although our library has primarily been developed for optimizing expression in N. benthamiana, we demonstrate the translatability of our promoters across distantly related species using a multiplexed reporter assay with barcoded transcripts. Our findings showcase the advantages of the PCONS library as an invaluable toolkit for plant synthetic biology.

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“…MiWRKY53, identified from mulberry (Morus indica var. K2) and induced by SA, functions as a positive regulator of plant defense response through SA-mediated mechanisms [55]. In this study, to know which of them, SA and Eth, could induce the expression of ShWRKY41, and was involved in wild tomato LA1777 against On-lz, the expression level of genes, PAL4 and ICS, coding the key enzymes of SA biosynthesis, ACS3 and ACO2, coding the key enzymes of Eth biosynthesis, were analyzed using qRT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of WRKY41, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (Solanum habrochaites) LA1777

Lian

Zhang

et al. 2022

IJMS

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WRKYs, a large family of transcription factors, are involved in plant response to biotic and abiotic stresses, but the role of them in tomato resistance to Oidium neolycopersici is still unclear. In this study, we evaluate the role of WRKYs in powdery mildew-resistant wild tomato (Solanum habrochaites) LA1777 defense against O. neolycopersici strain lz (On-lz) using a combination of omics, classical plant pathology- and cell biology-based approaches. A total of 27 WRKYs, belonging to group I, II, and III, were identified as differentially expressed genes in LA1777 against On-lz. It was found that expression of ShWRKY41 was increased after Pseudomonas syringae pv. tomato (Pst) DC3000, On-lz and Botrytis cinerea B05 inoculation or ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) treatment. GUS staining of ShWRKY41 promoter indicated that the expression of ShWRKY41 could be induced by SA and ethylene. Furthermore, ShWRKY41 gene silencing reduced the resistance to On-lz infection by decreasing the generation of H2O2 and HR in LA1777 seedlings. Overall, our research suggests that ShWRKY41 plays a positive role in defense activation and host resistance to O. neolycopersici in wild tomato (S. habrochaites) LA1777.

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A salicylic acid inducible mulberry WRKY transcription factor, MiWRKY53 is involved in plant defence response

Cited by 18 publications

References 69 publications

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

Identification and expression analysis of WRKY superfamily genes under lychee downy blight stress in lychee

A Suite of Constitutive Promoters for Tuning Gene Expression in Plants

Identification and Characterization of WRKY41, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (Solanum habrochaites) LA1777

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