A salicylic acid‐induced rice (<i>Oryza sativa</i> L.) transcription factor OsWRKY77 is involved in disease resistance of <i>Arabidopsis thaliana</i>

Chinese cabbage (Brassica rapa L. ssp. pekinensis), an important vegetable crop, can succumb to diseases such as bacterial soft rot, resulting in significant loss of crop productivity and quality. Pectobacterium carotovorum ssp. carotovorum (Pcc) causes soft rot disease in various plants, including Chinese cabbage. To overcome crop loss caused by bacterial soft rot, a gene from Chinese cabbage was isolated and characterised in this study. We isolated the BrWRKY12 gene from Chinese cabbage, which is a group II member of the WRKY transcription factor superfamily. The 645-bp coding sequence of BrWRKY12 translates to a protein with a molecular mass of approximately 24.4 kDa, and BrWRKY12 was exclusively localised in the nucleus. Transcripts of BrWRKY12 were induced by Pcc infection in Brassica. Heterologous expression of BrWRKY12 resulted in reduced susceptibility to Pcc but not to Pseudomonas syringae pv. tomato in Arabidopsis. Defence-associated genes, such as AtPDF1.2 and AtPGIP2, were constitutively expressed in transgenic lines overexpressing BrWRKY12. The expression of AtWKRY12, which is the closest orthologue of BrWRKY12, was down-regulated by Pcc in Arabidopsis. However, the Atwrky12-2 mutants did not show any difference in response to Pcc, pointing to a difference in function of WRKY12 in Brassica and Arabidopsis. Furthermore, BrWRKY12 in Chinese cabbage also exhibited enhanced resistance to bacterial soft rot and increased the expression of defence-associated genes. In summary, BrWRKY12 confers enhanced resistance to Pcc through transcriptional activation of defence-related genes.

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“…We found the recently indentified NLS domain of Os WRKY77 (Lan et al . ) is conserved in Br WRKY12 (Fig. S1).…”

Section: Resultsmentioning

confidence: 98%

Overexpression of the Brassica rapa transcription factor WRKY12 results in reduced soft rot symptoms caused by Pectobacterium carotovorum in Arabidopsis and Chinese cabbage

et al. 2014

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“…TFs play a crucial role in the coordination of plant growth and development, as well in the response to different stresses because they are able to regulate the spatial expression of the many different genes that are contained in the promoter region motifs that are recognized by TFs [ 53 ]. Based on the experimental data that describes the role the regulation of gene expression in model species [ 54 , 55 ], it is possible to screen the promoter region of any gene to identify which TFs are able to influence its expression [ 56 , 57 , 58 ]. Knowledge about which TFs can activate the gene expression allows the function of the gene that was analyzed in plant development program or response to external factors to be predicted.…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of the Genes Encoding Proteins that Are Involved in the Biosynthesis of the RMS/MAX/D Pathway Revealed New Roles of Strigolactones in Plants

Marzec

Muszyńska

2015

IJMS

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Strigolactones were described as a new group of phytohormones in 2008 and since then notable large number of their functions has been uncovered, including the regulation of plant growth and development, interactions with other organisms and a plant’s response to different abiotic stresses. In the last year, investigations of the strigolactone biosynthesis pathway in two model species, Arabidopsis thaliana and Oryza sativa, resulted in great progress in understanding the functions of four enzymes that are involved in this process. We performed in silico analyses, including the identification of the cis-regulatory elements in the promoters of genes encoding proteins of the strigolactone biosynthesis pathway and the identification of the miRNAs that are able to regulate their posttranscriptional level. We also searched the databases that contain the microarray data for the genes that were analyzed from both species in order to check their expression level under different growth conditions. The results that were obtained indicate that there are universal regulations of expression of all of the genes that are involved in the strigolactone biosynthesis in Arabidopsis and rice, but on the other hand each stage of strigolactone production may be additionally regulated independently. This work indicates the presence of crosstalk between strigolactones and almost all of the other phytohormones and suggests the role of strigolactones in the response to abiotic stresses, such as wounding, cold or flooding, as well as in the response to biotic stresses.

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“…Chitinase, hydrolytic enzymes in plants, showed in vitro antifungal activity (Karmakar et al, 2016) and many OsWRKY transcription factor were also involved in defence mechanisms in rice (Peng et al, 2016). Pathogenesis-related genes were upregulated by enhanced expression of several OsWRKYs, including OsWRKY77 (Jimmy and Babu, 2015), a transcription factor reported to play important roles in plant pathogen responses by activating the salicylic acid (SA) pathway (Lan et al, 2013). Consistent with the results of RNA-seq, transcriptional expression of two chitinase genes (CHIT7 and CHIT8) and two WRKY genes (OsWRKY77 and OsWRKY79) were confirmed elevated significantly by real-time PCR in spl30-1 and spl30-2 compared to wildtype ( Figure S9C).…”

Section: The Ups-mediated Degradation Of Spl30 N343ymentioning

confidence: 99%

OsACL‐A2 negatively regulates cell death and disease resistance in rice

Ruan

Hua

Zhao

et al. 2019

Plant Biotechnology Journal

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Summary ATP ‐citrate lyases ( ACL ) play critical roles in tumour cell propagation, foetal development and growth, and histone acetylation in human and animals. Here, we report a novel function of ACL in cell death‐mediated pathogen defence responses in rice. Using ethyl methanesulphonate ( EMS ) mutagenesis and map‐based cloning, we identified an Oryza sativa ACL ‐A2 mutant allele, termed spotted leaf 30‐1 ( spl30‐1 ), in which an A‐to‐T transversion converts an Asn at position 343 to a Tyr (N343Y), causing a recessive mutation that led to a lesion mimic phenotype. Compared to wild‐type plants, spl30‐1 significantly reduces ACL enzymatic activity, accumulates high reactive oxygen species and increases degradation rate of nuclear deoxyribonucleic acids. CRISPR /Cas9‐mediated insertion/deletion mutation analysis and complementation assay confirmed that the phenotype of spl30‐1 resulted from the defective function of Os ACL ‐A2 protein. We further biochemically identified that the N343Y mutation caused a significant degradation of SPL 30 N343Y in a ubiquitin‐26S proteasome system ( UPS )‐dependent manner without alteration in transcripts of Os ACL ‐A2 in spl30‐1 . Transcriptome analysis identified a number of up‐regulated genes associated with pathogen defence responses in recessive mutants of Os ACL ‐A2, implying its role in innate immunity. Suppressor mutant screen suggested that Os SL , which encodes a P450 monooxygenase protein, acted as a downstream key regulator in spl30‐1 ‐mediated pathogen defence responses. Taken together, our study discovered a novel role of Os ACL ‐A2 in negatively regulating innate immune responses in rice.

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A salicylic acid‐induced rice (Oryza sativa L.) transcription factor OsWRKY77 is involved in disease resistance of Arabidopsis thaliana

Cited by 45 publications

References 62 publications

Overexpression of the Brassica rapa transcription factor WRKY12 results in reduced soft rot symptoms caused by Pectobacterium carotovorum in Arabidopsis and Chinese cabbage

Overexpression of the Brassica rapa transcription factor WRKY12 results in reduced soft rot symptoms caused by Pectobacterium carotovorum in Arabidopsis and Chinese cabbage

In Silico Analysis of the Genes Encoding Proteins that Are Involved in the Biosynthesis of the RMS/MAX/D Pathway Revealed New Roles of Strigolactones in Plants

OsACL‐A2 negatively regulates cell death and disease resistance in rice

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