A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids

Filipits, Martin; Simon, Manuel; Rapatz, Winfried; Hamilton, Barbara; Ruis, Helmut

doi:10.1016/0378-1119(93)90513-3

Cited by 78 publications

(72 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SPS18/19 Promoter Region Is Unidirectionally Activated under Oleate Conditions-Oleate response elements, best described as palindromic CGG triplets spaced by 15-18 base pairs, mediate the transcriptional regulation of a number of peroxisomal protein-encoding genes, including POX1 (51), FOX3 (52), CTA1 (28), and PMP27 (53). The smallest element capable of relaying the fatty acid signal, a single ORE half-site (5Ј-CGGNNNTNA-3Ј), is sufficient for bi-directional induction (28).…”

Section: ) Xbai (X) a 189-base Pair Fragment Within The Sps19 Open mentioning

confidence: 99%

See 1 more Smart Citation

The Saccharomyces cerevisiae Peroxisomal 2,4-Dienoyl-CoA Reductase Is Encoded by the Oleate-inducible GeneSPS19

Gurvitz

Rottensteiner

Kilpeläinen

et al. 1997

Journal of Biological Chemistry

Self Cite

128

View full text Add to dashboard Cite

␤-Oxidation is compartmentalized in mammals into both mitochondria and peroxisomes. Fatty acids with double bonds at even-numbered positions require for their degradation the auxiliary enzyme 2,4-dienoyl-CoA reductase, and at least three isoforms, two mitochondrial and one peroxisomal, exist in the rat. The Saccharomyces cerevisiae Sps19p is 34% similar to the human and rat mitochondrial reductases, and an SPS19 deleted strain was unable to utilize petroselineate (cis-C 18:1(6) ) as the sole carbon source, but remained viable on oleate (cis-C 18:1(9) ). Sps19p was purified to homogeneity from oleate-induced cells and the homodimeric enzyme (native molecular weight 69,000) converted 2,4-hexadienoyl-CoA into 3-hexenoyl-CoA in an NADPH-dependent manner and therefore contained 2,4-dienoyl-CoA reductase activity. Antibodies raised against Sps19p decorated the peroxisomal matrix of oleate-induced cells. SPS19 shares with the sporulation-specific SPS18 a common promoter region that contains an oleate response element. This element unidirectionally regulates transcription of the reductase and is sufficient for oleate induction of a promoterless CYC1-lacZ reporter gene. SPS19 is dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate.

show abstract

Section: ) Xbai (X) a 189-base Pair Fragment Within The Sps19 Open mentioning

confidence: 99%

“…The smallest element capable of relaying the fatty acid signal, a single ORE half-site (5Ј-CGGNNNTNA-3Ј), is sufficient for bi-directional induction (28). Previous nucleotide analysis of the shared SPS18/19 promoter region had identified potential ORE half-sites (Fig.…”

Section: ) Xbai (X) a 189-base Pair Fragment Within The Sps19 Open mentioning

confidence: 99%

The Saccharomyces cerevisiae Peroxisomal 2,4-Dienoyl-CoA Reductase Is Encoded by the Oleate-inducible GeneSPS19

Gurvitz

Rottensteiner

Kilpeläinen

et al. 1997

Journal of Biological Chemistry

Self Cite

128

View full text Add to dashboard Cite

show abstract

“…The 3PORE:CYC1-lacZ reporter constructs were made essentially as described for pAG244 (Gurvitz et al, 1997). The Sal I-delineated oligonucleotides defining the 3Ј ORE half-site and a neighbouring GC-and A-rich region (oligonucleotides 3PORE1 and 2) were annealed and ligated into the XhoI site of the integrative vector pMF6 (Filipits et al, 1993), a derivative of pLG669Z (Guarente and Ptashne, 1981) to produce pAG419 and pAG420. Polymerase chain reaction (PCR) using the CYC1 oligonucleotide and either of the two 3PORE oligonucleotides (Table 2) at an annealing temperature of 45ЊC demonstrated that the ORE half-site in pAG419 (3PORE-d) was distal to the reporter gene, whereas in pAG420 (3PORE-p) it was proximal.…”

Section: Construction Of Gene Fusionsmentioning

confidence: 99%

“…The ⌬pox1::LEU2 fragment was excised from this plasmid and used to disrupt the POX1 gene in strain BJ1991. The resultant strain was transformed with the YEplac24 plasmid containing the TRP1 selectable marker (Gietz and Sugino, 1988) and mated with the MF24-transformed (Filipits et al, 1993) wild-type and ⌬pox1 strains A232-4A and A232-4A-33 (Dmochowska et al, 1990) respectively, resulting in the wild-type and ⌬pox1 diploids yAG652 and yAG651.…”

Section: Heterologous Expression Of Ha-tagged Pip2pmentioning

confidence: 99%

“…OREs are known to mediate the induction of a number of S. cerevisiae genes encoding peroxisomal proteins, including catalase A (CTA1; Filipits et al, 1993), acyl-CoA oxidase (POX1 ; Wang et al, 1994) and thiolase (FOX3 ; Einerhand et al, 1993), and are the binding target for Pip2p and Oaf1p (Luo et al, 1996;Rottensteiner et al, 1996;Karpichev et al, 1997). Peroxisomes and their biogenesis are the subject of intense research, not least because these organelles and their proliferation have been linked to several human genetic disorders, including Zellweger syndrome (Kaiser and Kramar, 1988;Masters and Crane, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of the yeast SPS19 gene encoding peroxisomal 2,4‐dienoyl‐CoA reductase by the transcription factors Pip2p and Oaf1p: β‐oxidation is dispensable for Saccharomyces cerevisiae sporulation in acetate medium

Gurvitz

Rottensteiner

et al. 1997

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

SummaryThe yeast SPS19 gene encoding the peroxisomally targeted 2,4-dienoyl-CoA reductase shares its promoter region (291 bp) with the sporulation-specific gene SPS18. SPS19 is induced during sporulation in diploids but to a lesser extent than SPS18 ; under oleate induction conditions, SPS19, but not SPS18, is transcribed via an oleate response element (ORE) independently of ploidy or sporulation. The SPS19 ORE is the binding target of the Pip2p and Oaf1p transcription factors, and an SPS19-lacZ reporter gene, which is highly expressed in oleate-induced cells, is not activated in haploids devoid of either protein.We examined the expression of CYC1-lacZ reporter constructs carrying the SPS19 and CTA1 OREs in diploids propagated under sporulation conditions and have shown that OREs are not sufficient for heterologous expression during yeast development. In addition, diploids deleted at either PIP2 or OAF1 demonstrated abundant ascosporogenesis, indicating that these genes are not essential for sporulation. A ⌬pex6 strain lacking peroxisomal structures and one devoid of fatty acyl-CoA oxidase (⌬pox1), the first step in fungal ␤-oxidation, were both proficient for sporulation and, hence, ␤-oxidation and the peroxisomal compartment containing it are dispensable for meiotic development.

show abstract

Independent regulation of full-length and 5′-truncatedPAS5 mRNAs inSaccharomyces cerevisiae

Liu

Dieckmann

1996

Yeast

View full text Add to dashboard Cite

Peroxisome assembly in Saccharomyces cerevisiae requires the products of several genes. In this report, the PAS5 gene has been characterized. The gene is on the left arm of chromosome X and encodes a polypeptide with similarity to the mammalian peroxisome assembly factor‐1 (PAF‐1). Two different length transcripts are produced from the yeast PAS5 gene. The longer mRNAs encompass an open reading frame, while the shorter transcripts initiate 46–110 base pairs downstream of the first in‐frame AUG. The longer transcripts are induced four‐fold on medium containing fatty acids as the sole carbon source, while the shorter transcripts are induced up to ten‐fold on medium containing glycerol as a carbon source. The full‐length coding sequence encodes a protein with a calculated molecular weight of 30·7 kDa. A protein of 25 kDa could be translated from the shorter transcripts and would lack a very acidic domain found in the amino‐terminal extension of the longer protein. The common portion of the proteins is very basic; the calculated pI of the longer polypeptide is 9·02 and that of the shorter protein is 10·06. The PAS5 GenBank accession number is M86538.

show abstract

A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids

Cited by 78 publications

References 28 publications

The Saccharomyces cerevisiae Peroxisomal 2,4-Dienoyl-CoA Reductase Is Encoded by the Oleate-inducible GeneSPS19

The Saccharomyces cerevisiae Peroxisomal 2,4-Dienoyl-CoA Reductase Is Encoded by the Oleate-inducible GeneSPS19

Regulation of the yeast SPS19 gene encoding peroxisomal 2,4‐dienoyl‐CoA reductase by the transcription factors Pip2p and Oaf1p: β‐oxidation is dispensable for Saccharomyces cerevisiae sporulation in acetate medium

Independent regulation of full-length and 5′-truncatedPAS5 mRNAs inSaccharomyces cerevisiae

Contact Info

Product

Resources

About