A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions.

Marchler, Gabriele H.; Schüller, Christoph; Adam, Gerhard; Ruis, Helmut

doi:10.1002/j.1460-2075.1993.tb05849.x

Cited by 457 publications

(474 citation statements)

References 40 publications

Supporting

Mentioning

449

Contrasting

Unclassified

Order By: Relevance

“…Genes regulated by the HOG pathway contain a positive stress promoter element (STRE) with core consensus sequence CCCCT or AGGGG [22,23]. A single STRE-like sequence is found in the ENA1 promoter region (-651 from ATG) and it could mediate part of the effects observed in this work.…”

Section: ~ Discussionmentioning

confidence: 70%

Multiple transduction pathways regulate the sodium‐extrusion gene PMR2/ENA1 during salt stress in yeast

1996

View full text Add to dashboard Cite

The yeast PMR21£NAI gene encodes an ATPase involved in sodium extrusion and induced by NaCI. At low salt concentrations (0.3 1VID induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stre&~. At high salt concentrations (0.8 M) induction i~ mediated by the protein phosphatase caleineurin and is specific for sodium. Protein kinase A and Sis2ptHal3p modulate PMR21£NAI expression as negative and positive factors, respectively but Sis2p/Hal3p does not perticipate in the transduction of the salt signal. Salt stress dect'eases the level of cAMP and the resulting decrease in protein kinase A activity may contribute t6 HOGmediated Induction.Key words: Signal transduction; Salt stress; MAP kinase; Calcineurin; cAMP; Saccharomyces cerevisiae 1. Introductit, n Living cells can withstand a variety of stress conditions such as extreme temperatures, water deficit, high salt concentrations, starvation etc. Under these circumstances organisms modify their cellular machinery in order to counteract the deleterious effect of damaging agents. This constitutes a stress response and involves modulation of enzymatic activities as well as changes in gene expression [1,2]. In ~'ecent years considerable interest has been raised by the mechanisms of plant tolerance to drought and salinity, two major factors limiting agricultural productivity [3]. The signal transduction pathways operating during osmotic and salt stress are only partially understood and this gap of knowledge is a limiting factor for urgent biotechnological applications [2].The yeast Saccharomyces cerevisiae can be utilized as a convenient model system in salinity studies [2]. Salt stress has two major harmful effects for cells: the loss of turgor pressure and the toxicity of Na ÷ ions to cellular metabolism. The adaptation of yeast to these conditions requires the modification of the plasma membrane transport systems to exclude Na + ions from the cytosol and the onset of glycerol synthesis to restore turgor pressure. This adaptation is largely based on changes in the expression of key genes such as PMR21ENAI (in the following referred to as ENAI), encoding an ATPase involved in sodium extrusion [4], and GPDI, encoding~a dehydrogenase involved in gljcerol synthesis [5]. Many other genes are also induced by saP, stress whose contribution to the adaptatio,a, if any, is not well understood [2].In the present work we have investigated the environmental signals and tran,,~lucing pathways mediating the induction of *Corresponding author'. Fax: (34) (6) Materials and methodsYPD and synthetic medium were prepared as described [12]. YPD contained 2% glucose, 1% yeast extract and 2% peptone. Synthetic medium contained 2% glucose, 0.7% yeast nitrogen base without amino acids (Difco), 50 mM MES [2.(N-morpholino) ethanosulfonic acid] adjusted to pH 6.0 with Tris and the amino acids, purine and pyrimidine bases required by the strains.Strains YPH499 (MATs ura3 leu2 his3 trpl lys2 ado2) and JBY10 (isogonic to YPH499 with hogl.Al:: TRPI) ...

show abstract

Section: ~ Discussionmentioning

confidence: 70%

Multiple transduction pathways regulate the sodium‐extrusion gene PMR2/ENA1 during salt stress in yeast

1996

View full text Add to dashboard Cite

show abstract

“…To counteract the positive regulatory effect exerted by Yap1p and Msn2p is the Ras-cAMP-PKA pathway, which has been previously shown to negatively regulate both Yap1p (Fernandes et al, 1997) and STRE-driven induction (Gorner et al, 1998;Marchler, 1993).…”

Section: Introductionmentioning

confidence: 99%

YAP4 gene expression is induced in response to several forms of stress in Saccharomyces cerevisiae

Nevitt

Rodrigues-Pousada

2004

Yeast

View full text Add to dashboard Cite

Exposure of Saccharomyces cerevisiae to several environmental insults, including conditions of oxidative, heavy metal, metalloid and heat stress, induces the expression of the YAP4 gene, previously shown to play a role in the response to hyperosmotic stress. Expression analyses in several mutant strains under pro-oxidant conditions have determined that YAP4 is regulated by the transactivators Yap1p and Msn2p. Mutation of either the Yap1p-response element (YRE), located at −517 bp from the ATG, or the most proximal stress response element (STRE) at −430 bp, is shown to strongly compromise YAP4 gene expression under these conditions. Furthermore, these two mutations in combination lead to a severe depletion of detectable mRNA levels, indicating interplay between the transcription factors Yap1p and Msn2p in the regulation of YAP4 transcription. Transcriptional activation of this gene reflects a concomitant increase in Yap4p protein levels that appear phosphorylated upon stress and negatively regulated by protein kinase A. Yap4p amino acid residues Ser89, Ser196 and Thr241 are shown to be required for protein phosphorylation and/or protein stability.

show abstract

“…However, a subset of genes that is characteristic for this phase was already induced when glucose and all other nutrients were still available in the medium, and expression was induced far in advance of other so-called diauxic and stationary phase events (Herman, 2002;Werner-Washburne et al, 1993, 1996. Examples include genes involved in the synthesis of reserve carbohydrates (for review, see Francois and Parrou, 2001) and the general stress-response gene CTT1 (Marchler et al, 1993). Expression of the hexose transporter HXT5 was also induced when still ample glucose was present in the medium (Verwaal et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

Verwaal

Arako

Kapur

et al. 2004

Yeast

View full text Add to dashboard Cite

Hexose transporter (Hxt) proteins transport glucose across the plasma membrane in the yeast Saccharomyces cerevisiae. Recently, we have shown that expression of HXT5 is regulated by the growth rate of the cells. Because gene expression is regulated by binding of specific transcription factors to regulatory elements in the promoters of genes, the presence of putative regulatory elements in the promoter of HXT5 was determined by computer-assisted analysis. This revealed the presence of two putative stress-responsive elements (STREs), one putative post-diauxic shift (PDS) element and two putative Hap2/3/4/5p (HAP) complex binding elements. The involvement of these elements was studied by using mutations in a HXT5 promoter-LacZ fusion construct. Growth during various conditions that result in low growth rates of yeast cells revealed that the STRE most proximal to the translation initiation site seemed to be involved in particular in regulation of HXT5 expression during growth at decreased growth rates. In addition, the HAP elements seemed to be required during growth on non-fermentable carbon sources. The PDS element and, to a lesser extent, the other STRE showed particular involvement in regulation of HXT5 expression during growth on ethanol. Finally, it was shown that the PKA pathway, which is known to be involved in expression of STRE-regulated genes, was also involved in regulation of HXT5 expression. A possible mechanism by which expression of HXT5 could be regulated by the transcriptional regulatory elements in the promoter is discussed.

show abstract

A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions.

Cited by 457 publications

References 40 publications

Multiple transduction pathways regulate the sodium‐extrusion gene PMR2/ENA1 during salt stress in yeast

Multiple transduction pathways regulate the sodium‐extrusion gene PMR2/ENA1 during salt stress in yeast

YAP4 gene expression is induced in response to several forms of stress in Saccharomyces cerevisiae

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

Contact Info

Product

Resources

About