A routine assay procedure for monoamine oxidase and its application to human blood platelets

Demisch, L.; Bochnik, Hans J.; Seiler, Nikolaus

doi:10.1016/0009-8981(76)90348-x

Cited by 19 publications

(2 citation statements)

References 26 publications

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“…using Instagel (Packard Instruments, Warrenville, Illinois) as scintillant. By using a multiple column chromatographic device, 48 coluinns can be operated in parallel [45] . This allows the estimation of A4ADC in at least 96 samples per working day.The labeled tryptophan was purified prior to use by using the same columns.…”

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confidence: 99%

Metabolic inhibitors and subcellular distribution of GABA

Sarhan

Seiler

1979

J of Neuroscience Research

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Experimental procedures are described which are believed to yield results that reflect, within certain limits, the in vivo changes of the size of the GABA pool in nerve endings in comparison with those of all other GABA pools. Two irreversible GABA-T inhibitors, vinyl GABA and acetylenic GABA, two GAD inhibitors, 3-mercaptopropionic acid and pyridoxal phosphate glutamyl-gamma-hydrazone, and di-n propylacetate, a clinically useful anticonvulsant, have been studied to determine their effects on GABA compartmentalization in mouse brain cortex. The changes elicited by these drugs in subcellular fractions of brain cortex homogenates support the notion that measurement of amino acid concentrations in crude synaptosomal fractions and in supernatant fractions under controlled conditions allow one to draw conclusions about relative changes of pool sizes in vivo. In particular this work showed that a specific increase in the concentration of GABA within the nerve endings is more important than a large increase of total brain GABA as a means of decreasing susceptibility to a variety of chemically or physically induced seizures.

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confidence: 99%

Metabolic inhibitors and subcellular distribution of GABA

Sarhan

Seiler

1979

J of Neuroscience Research

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“…Aliquots of plasmas A and B were removed for measuring platelet amounts and sizes, MOA-B activity and 5-HT concentrations. The median volurne distribution and platelet counts were measured electronically and MAO activity calculated according to Demisch et al (1976) using p-tyramine as substrate. 5-HT was chromatographed by HPLC (5~m ODS column) and detected by fluorescence (Mellord and Barchas, 1982;Peat and Gibb, 1983).…”

Section: Methods and Resultsmentioning

confidence: 99%

Methodical Problems with α₂-Receptor-binding Studies on Membrane Fragments of Blood Platelets in Clinical Investigations

Syha¹

1985

Pharmacopsychiatry

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Two different subtypes of antimitochondrial antibodies are associated with primary biliary cirrhosis: Identification and characterization by radioimmunoassay and immunoblotting

et al. 1987

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Antimitochondrial antibodies from patients with primary biliary cirrhosis react with different mitochondrial polypeptides as demonstrated by Western blots. The IgG fractions of a patient with primary biliary cirrhosis Stage I reacting exclusively with a pair of polypeptides at 48,000 daltons (p 48) on Western blot and from a patient with Stage III primary biliary cirrhosis reacting exclusively with a single 62,000 dalton polypeptide (p 62) were labeled with 125I; two radioimmunoassays were established detecting antimitochondrial antibodies against p 62 and p 48, respectively. Autologous sera blocked the assay, but the two reference sera did not block each other. Fourteen of 40 patients with primary biliary cirrhosis reacted with p 62, 6/40 with p 48 and 20 sera with both antigens. Sera from 200 patients with various hepatic and nonhepatic diseases were negative for anti-p 62 and anti-p 48. This collection of sera included 5 patients with nonhepatic autoimmune disorders, 3 with drug-induced pseudolupus syndrome and 2 with syphilis II, which were positive for antimitochondrial antibodies by immunofluorescence. Mitochondrial autoantigens p 62 and p 48 were both localized on mitoplasts, presumably inner mitochondrial membranes; they were thermolabile, trypsin- and chymotrypsin-sensitive, but resistant to DNAase, RNAase and neuraminidase treatments. In cesium chloride density gradients, p 62 floated at 1.28 gm per cm3 and p 48 at 1.30 gm per cm3. Thus, radioimmunoassays have been developed that specifically detect two distinct primary biliary cirrhosis-specific subtypes of antimitochondrial antibodies: anti-p 62 and anti-p 48. All primary biliary cirrhosis sera were positive for at least one of these antimitochondrial antibodies subtypes.(ABSTRACT TRUNCATED AT 250 WORDS)

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A routine assay procedure for monoamine oxidase and its application to human blood platelets

Cited by 19 publications

References 26 publications

Metabolic inhibitors and subcellular distribution of GABA

Metabolic inhibitors and subcellular distribution of GABA

Methodical Problems with α₂-Receptor-binding Studies on Membrane Fragments of Blood Platelets in Clinical Investigations

Two different subtypes of antimitochondrial antibodies are associated with primary biliary cirrhosis: Identification and characterization by radioimmunoassay and immunoblotting

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A routine assay procedure for monoamine oxidase and its application to human blood platelets

Cited by 19 publications

References 26 publications

Metabolic inhibitors and subcellular distribution of GABA

Metabolic inhibitors and subcellular distribution of GABA

Methodical Problems with α2-Receptor-binding Studies on Membrane Fragments of Blood Platelets in Clinical Investigations

Two different subtypes of antimitochondrial antibodies are associated with primary biliary cirrhosis: Identification and characterization by radioimmunoassay and immunoblotting

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Methodical Problems with α₂-Receptor-binding Studies on Membrane Fragments of Blood Platelets in Clinical Investigations