A ROS-dependent mechanism to drive progression through S phase

Kirova, Dilyana Georgieva; Vorhauser, Julia; Zerjatke, Thomas; Leung, Jacky K.; Glauche, Ingmar; Mansfeld, Jörg

doi:10.1101/2022.03.31.486607

Cited by 3 publications

(2 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results show that 62 key targets were mainly gathered in melanoma cells. By intersecting the key cluster and the intersection of drug-related genes and melanoma-related genes, we finally identified three crucial targets, EGFR, ERBB2, and CDK2, which are all protein serine/threonine kinases and are involved in cell cycle regulation (Lo and Hung, 2006;Hirai et al, 2017;Kirova et al, 2022). It has been reported that cinobufagin can inhibit the EGFR-CDK2 signaling pathway in hepatocellular carcinoma, which is consistent with our predicted results (Yang et al, 2021).…”

Section: Discussionsupporting

confidence: 84%

Mechanisms underlying the therapeutic effects of cinobufagin in treating melanoma based on network pharmacology, single-cell RNA sequencing data, molecular docking, and molecular dynamics simulation

Yang,

Cheng,

2024

Front. Pharmacol.

View full text Add to dashboard Cite

Malignant melanoma is one of the most aggressive of cancers; if not treated early, it can metastasize rapidly. Therefore, drug therapy plays an important role in the treatment of melanoma. Cinobufagin, an active ingredient derived from Venenum bufonis, can inhibit the growth and development of melanoma. However, the mechanism underlying its therapeutic effects is unclear. The purpose of this study was to predict the potential targets of cinobufagin in melanoma. We gathered known and predicted targets for cinobufagin from four online databases. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were then performed. Gene expression data were downloaded from the GSE46517 dataset, and differential gene expression analysis and weighted gene correlation network analysis were performed to identify melanoma-related genes. Using input melanoma-related genes and drug targets in the STRING online database and applying molecular complex detection (MCODE) analysis, we identified key targets that may be the potential targets of cinobufagin in melanoma. Moreover, we assessed the distribution of the pharmacological targets of cinobufagin in melanoma key clusters using single-cell data from the GSE215120 dataset obtained from the Gene Expression Omnibus database. The crucial targets of cinobufagin in melanoma were identified from the intersection of key clusters with melanoma-related genes and drug targets. Receiver operating characteristic curve (ROC) analysis, survival analysis, molecular docking, and molecular dynamics simulation were performed to gain further insights. Our findings suggest that cinobufagin may affect melanoma by arresting the cell cycle by inhibiting three protein tyrosine/serine kinases (EGFR, ERBB2, and CDK2). However, our conclusions are not supported by relevant experimental data and require further study.

show abstract

Section: Discussionsupporting

confidence: 84%

Mechanisms underlying the therapeutic effects of cinobufagin in treating melanoma based on network pharmacology, single-cell RNA sequencing data, molecular docking, and molecular dynamics simulation

Yang,

Cheng,

2024

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Moreover, cellular metabolism and its redox potential associated play a crucial role in cell cycle regulation. In mitotic cells, the progression of cell division is accompanied by an increase in ROS levels, especially in the mitochondria [ 204 ]. ROS influence cell cycle progression in a context-dependent manner via phosphorylation and ubiquitination of CDKs and cell cycle regulatory molecules [ 205 ].…”

Section: Impact Of Ros On Biological Events In Agingmentioning

confidence: 99%

ROS: Basic Concepts, Sources, Cellular Signaling, and its Implications in Aging Pathways

Almeida

Oliveira

Pontes

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Reactive oxygen species (ROS) are bioproducts of cellular metabolism. There is a range of molecules with oxidizing properties known as ROS. Despite those molecules being implied negatively in aging and numerous diseases, their key role in cellular signaling is evident. ROS control several biological processes such as inflammation, proliferation, and cell death. The redox signaling underlying these cellular events is one characteristic of the new generation of scientists aimed at defining the role of ROS in the cellular environment. The control of redox potential, which includes the balance of the sources of ROS and the antioxidant system, implies an important target for understanding the cells’ fate derived from redox signaling. In this review, we summarized the chemical, the redox balance, the signaling, and the implications of ROS in biological aging.

show abstract

Navigating the redox landscape: reactive oxygen species in regulation of cell cycle

Mackova,

Raudenska,

Polanska

et al. 2024

Redox Report

View full text Add to dashboard Cite

A ROS-dependent mechanism to drive progression through S phase

Cited by 3 publications

References 100 publications

Mechanisms underlying the therapeutic effects of cinobufagin in treating melanoma based on network pharmacology, single-cell RNA sequencing data, molecular docking, and molecular dynamics simulation

Mechanisms underlying the therapeutic effects of cinobufagin in treating melanoma based on network pharmacology, single-cell RNA sequencing data, molecular docking, and molecular dynamics simulation

ROS: Basic Concepts, Sources, Cellular Signaling, and its Implications in Aging Pathways

Navigating the redox landscape: reactive oxygen species in regulation of cell cycle

Contact Info

Product

Resources

About