A ROS-dependent and Caspase-3-mediated apoptosis in sheep bronchial epithelial cells in response to Mycoplasma Ovipneumoniae infections

Xue, Ding; Li, Yanan; Jiang, Zhongjia; Deng, Guangcun; Li, Min; Liu, Xiaoming; Wang, Yujiong

doi:10.1016/j.vetimm.2017.04.004

Cited by 13 publications

(21 citation statements)

References 39 publications

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“…Sheep infected with M. ovipneumoniae show respiratory disorders, runny noses, weight loss, growth retardation, and primary infection within 1–3 months, depending on the age of the sheep (Besser et al 2017 ). The control and prevention of M. ovipneumoniae is difficult and has caused great economic losses to the sheep industry in many countries (Giangaspero et al 2012 ; Xue et al 2017 ). In addition, upon M. ovipneumoniae infection, sheep become susceptible to other diseases.…”

Section: Introductionmentioning

confidence: 99%

Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae

Zhang

Cao

Zhu

et al. 2019

World J Microbiol Biotechnol

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In order to establish a rapid detection method for Mycoplasma ovipneumoniae , this study used the loop-mediated isothermal amplification (LAMP) technique to carry out nucleic acid amplification and chromatographic visualization via a lateral flow dipstick (LFD) assay. The M. ovipneumoniae elongation factor TU gene ( EF-TU ) was detected using a set of specific primers designed for the EF-TU gene, and the EF-TU FIP was detected by biotin labeling, which was used in the LAMP amplification reaction. The digoxin-labeled probe specifically hybridized with LAMP products, which were visually detected by LFD. Here, we established the M. ovipneumoniae LAMP-LFD rapid detection method and tested the specificity, sensitivity, and clinical application of this method. Results showed that the optimized LAMP performed at 60 °C for 60 min, and LFD can specifically and visually detect M. ovipneumoniae with a minimum detectable concentration at 1.0 × 10 2 CFU/mL. The sensitivity of LAMP-LFD was 1000 times that of the conventional PCR detection methods, and the clinical lung tissue detection rate was 86% of 50 suspected sheep infected with M. ovipneumoniae . In conclusion, LAMP-LFD was established in this study to detect M. ovipneumoniae , a method that was highly specific, sensitive, and easy to operate, and provides a new method for the prevention and diagnosis of M. ovipneumoniae infection. Electronic supplementary material The online version of this article (10.1007/s11274-019-2601-5) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae

Zhang

Cao

Zhu

et al. 2019

World J Microbiol Biotechnol

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“…The pathogenesis of M. ovipneumoniae involves a polysaccharide capsule, which could promote adherence of the organisms to the ciliated epithelium, thus promoting bacterial colonization [ 12 , 13 ]. Furthermore, the polysaccharide capsule may have cytotoxic effects on the epithelial cells, inducing apoptosis [ 14 , 15 ], which would also contribute to the pathogenesis of the disease. Mycoplasma spp.…”

Section: Introductionmentioning

confidence: 99%

Bronchopneumonia in Swedish lambs: a study of pathological changes and bacteriological agents

2018

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BackgroundOne of the most common post-mortem inspection finding of sheep and lambs in Sweden, following routine slaughter is pneumonia and its prevalence is increasing. To our knowledge, the aetiology of pneumonia in lambs is not well-known for Swedish conditions. Chronic bronchopneumonia, also known as “atypical” or chronic non-progressive pneumonia, is a common disease worldwide, affecting lambs up to 12 months old. It is therefore of interest to elucidate if this disease complex is also a common cause of pneumonia among Swedish lambs. Chronic bronchopneumonia has a characteristic macroscopic and histopathologic appearance, and Mycoplasma ovipneumoniae is the microbial agent most frequently found. Although this bacterium is important for the pathogenesis, multiple agents are presumed to be involved. The aim of this study was to describe the macroscopic and histopathologic lung lesions in routinely slaughtered lambs with pneumonia, and to determine the bacterial agents involved.ResultsA total of 41 lungs with gross lesions consistent with pneumonia were examined. Of these, 35 lungs displayed the typical gross appearance of chronic bronchopneumonia, with several or all of the characteristic histological features. M. ovipneumoniae was detected in 83% of the 35 lungs and Mannheimia haemolytica was isolated in 71%. Pneumonia associated with M. ovipneumoniae could be correlated to specific gross lesions consistent with the gross description of chronic bronchopneumonia in lambs.ConclusionIn this study, chronic bronchopneumonia was the most common lung disease in routinely slaughtered Swedish lambs. This diagnosis was based on the characteristic macroscopic and histopathologic pulmonary findings and the frequent presence of the bacterium M. ovipneumoniae. The macroscopic appearance of chronic bronchopneumonia could therefore be used during routine investigation of the lamb carcasses at slaughter, to determine the most likely cause of pneumonia.

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“…The lipopolysaccharide from Leptospira interrogans also causes macrophage apoptosis (Du et al, 2018a). M. ovipneumoniae caused apoptosis in sheep airway epithelial cells via ROS-induced pathways and the ERK-mediated mitochondrial pathway (Jiang et al, 2017; Li et al, 2016; Xue et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…M. ovipneumoniae has been found in goats (Livingston & Gauer, 1979; Mohan, Obwolo, & Hill, 1992; Rifatbegovic, Maksimovic, & Hulaj, 2011; Rong et al, 2014), bighorn sheep (Besser et al, 2014), moose, caribou, mule, white-tailed deer, and mule deer (Highland et al, 2018) besides sheep. Previous studies of the pathogenic mechanisms of M. ovipneumoniae showed that this bacterium caused direct cell death via the reactive oxygen species (ROS) and the the mitogen-activated protein kinase (MAPK) signaling–mediated mitochondrial apoptotic pathway (Li et al, 2016; Xue et al, 2017). Moreover, the M. ovipneumoniae capsular polysaccharide caused the apoptosis of sheep airway epithelial cells via the c-Jun N-terminal kinase (JNK)/P38 MAPK signaling pathway (Jiang et al, 2017; Weiser et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Strategies of Mycoplasma ovipneumoniae to evade immune clearance by alveolar macrophages

Gao

Zhang

et al. 2021

Preprint

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Evidences showed that M. ovipneumoniae might associate with the development and duration of chronic pneumonia. Moreover, sheep infected with M. ovipneumoniae are easily infected by other organisms, suggesting that M. ovipneumoniae may play an immunosuppressive role during infection. However, the mechanism is still poorly understood. The infection occurs in the airway, where resident alveolar macrophages first encounter M. ovipneumoniae. Therefore, primary alveolar macrophages (AMs) were collected from the lungs of healthy adult sheep, and the (iTRAQ) protein assay was used to investigate the immunosuppressive effects of M. ovipneumoniae on sheep AMs. The RAW264.7 cells were used to confirm the findings. The results showed that M. ovipneumoniae promoted higher expression of anti-apoptotic proteins and lower expression of apoptosis-related proteins in the infected AMs. Moreover, the number of infected AMs increased. However, M. ovipneumoniae reduced ATP levels in AMs and impaired late endosome maturation and phagolysosome fusion. Furthermore, M. ovipneumoniae inhibited the autophagy pathway via the Akt-mTOR axis in AMs. These findings indicated that M. ovipneumoniae had distinctive strategies to evade elimination caused by the AMs. The findings might explain the chronic infection and co-infection in sheep infected by M. ovipneumoniae.

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A ROS-dependent and Caspase-3-mediated apoptosis in sheep bronchial epithelial cells in response to Mycoplasma Ovipneumoniae infections

Cited by 13 publications

References 39 publications

Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae

Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae

Bronchopneumonia in Swedish lambs: a study of pathological changes and bacteriological agents

Strategies of Mycoplasma ovipneumoniae to evade immune clearance by alveolar macrophages

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