A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris)

Rodriguez‐Uribe, Laura; O’Connell, Mary A.

doi:10.1093/jxb/erj118

Cited by 94 publications

(64 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They include various transcription factors, protein kinases, protein phosphatases, response regulators, calmodulin-like protein, and ABI binding protein amongst others. bHLH, bZIP, EREBP, NAC, MPK7, ABI, GASA4, and CAM proteins are examples of signal transduction elements or transcription factor families that have been found to be responsive to drought stress in other species (ToledoOrtiz et al 2003;White and Broadley 2003;Rodriguez-Uribe and O'Connell 2006;Shen et al 2003;Olsen et al 2005;Meng et al 2009a, b). As expected, these gene families were also identified in our study, where not only families but homologs from the same family demonstrated unique expressional changes in response to water scarcity.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Zhang

et al. 2011

Plant Mol Biol Rep

View full text Add to dashboard Cite

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a vegetable crop with high water requirement and is adversely affected by drought during cultivation. A genome-wide transcription analysis in response to drought is essential to provide effective genetic engineering strategies to improve stress tolerance in crop plants. To gain a deeper understanding of the mechanisms of drought tolerance in B. rapa, we conducted the first genome-wide analysis of gene expression during a drought model using tag sequencing with a Solexa Illumina array. Leaf samples at four time points, 0 day, 1 day, 2 days, and 3 days after dehydration treatment, were taken from a B. rapa DH line, T12-19, and the corresponding RNA was used to construct the expression libraries. The total number of tags per library ranged from 5.6 to 7.6 million and, among these, 13,036, 12,472, 12,774, and 12,227 distinct clean tags for the four respective libraries were unambiguously mapped to a publicly available unigene database. The analysis of differentially expressed genes revealed that 1,092 genes were significantly altered in response to water deficit. In this set of genes, we found 37 transcription factors, 28 genes involved in signal transduction, and 61 water-and osmosensing-responsive genes. Overall, the tag sequencing analyses demonstrated a high degree of transcriptional complexity in response to dehydration stress and represents a considerable improvement over microarray data in the large-scale analysis of transcriptional changes.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Zhang

et al. 2011

Plant Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…Genomic DNA (10 or 20 lg) was digested separately with AvaI, BamHI, EcoRI, EcoRV, HindIII, PstI or XbaI; blots were prepared, hybridized and washed as described (Rodriguez-Uribe and O'Connell 2006). Probes for hybridization were prepared using cloned mtDNA genes: wheat for cox1, cotton for cox2 and cox3 (Wang 2008), maize for atp1 (atpA) (Braun and Levings 1985), atp6 (Dewey et al 1986), cob and rrn26 (26S rRNA) (Stern et al 1982), and petunia for atp9 (Young and Hanson 1987).…”

Section: Southern Blot Analysismentioning

confidence: 99%

RFLP analysis of mitochondrial DNA in two cytoplasmic male sterility systems (CMS-D2 and CMS-D8) of cotton

et al. 2009

View full text Add to dashboard Cite

Cytoplasmic male sterility (CMS) in higher plants is a maternally inherited trait and CMSassociated genes are known to be located in the mitochondrial genome. However, CMS-inducing genes in CMS-D2 and CMS-D8 of Upland cotton (Gossypium hirsutum L., AD1) are currently unknown. The objective of this study was to identify potential candidate DNA or gene sequences for CMS-D2 and CMS-D8 through restriction fragment length polymorphism (RFLP) analysis. Seven mtDNA gene probes and five restriction enzymes were first used to compare D2 (from G. harknessii Brandegee) and AD1 cytoplasms. With cox1, cox2, and atp1 as probes, RFLP polymorphisms were detected with one or more restriction enzyme digestions. The most notable difference was an additional fragment in the normal AD1 cytoplasm detected by cox2 in digests of three enzymes, and by cox1 and atp1 in digests with PstI. The RFLP analysis was then conducted among CMS-D2, CMS-D8 (from G. trilobum (DC.) Skovst.), and AD1 cytoplasms. Two probes from maize, atp1 and atp6, detected polymorphism among the different cytoplasmic lines. However, no difference in RFLP patterns was noted between male sterile (A) and restorer (R) lines with the D2 or D8 cytoplasm, indicating that the presence of the D2 or D8 restorer gene does not affect mtDNA organization in Upland cotton. The results demonstrate that RFLP using atp1 and atp6 as probes can distinguish the three cytoplasms. The atp1 and atp6 in CMS-D8 and these two genes together with cox1 and cox2 in CMS-D2 could be the candidates of CMS-associated genes in the mitochondrial genome, providing information for further molecular studies and developing PCR-based markers for the CMS cytoplasms in breeding. This research represents the first work using RFLP to analyze the genetic basis of CMS in cotton.

show abstract

“…In plants, like other transcription factor genes, members of the bZIP transcription factor family are also either expressed constitutively or in an organ-specific (Schindler et al, 1992a;Rodriguez-Uribe and O'Connell, 2006), stimulus-responsive (de Vetten and Ferl, 1995), development-dependent (Chern et al, 1996), and cellcycle-specific (Minami et al, 1993) manner. So far, over 120 bZIP transcription factors have been identified in different plants (Supplemental Table S1).…”

mentioning

confidence: 99%

Genomic Survey and Gene Expression Analysis of the Basic Leucine Zipper Transcription Factor Family in Rice

et al. 2007

View full text Add to dashboard Cite

The basic leucine (Leu) zipper (bZIP) proteins compose a family of transcriptional regulators present exclusively in eukaryotes. The bZIP proteins characteristically harbor a bZIP domain composed of two structural features: a DNA-binding basic region and the Leu zipper dimerization region. They have been shown to regulate diverse plant-specific phenomena, including seed maturation and germination, floral induction and development, and photomorphogenesis, and are also involved in stress and hormone signaling. We have identified 89 bZIP transcription factor-encoding genes in the rice (Oryza sativa) genome. Their chromosomal distribution and sequence analyses suggest that the bZIP transcription factor family has evolved via gene duplication. The phylogenetic relationship among rice bZIP domains as well as with bZIP domains from other plant bZIP factors suggests that homologous bZIP domains exist in plants. Similar intron/exon structural patterns were observed in the basic and hinge regions of their bZIP domains. Detailed sequence analysis has been done to identify additional conserved motifs outside the bZIP domain and to predict their DNA-binding site specificity as well as dimerization properties, which has helped classify them into different groups and subfamilies, respectively. Expression of bZIP transcription factor-encoding genes has been analyzed by full-length cDNA and expressed sequence tag-based expression profiling. This expression profiling was complemented by microarray analysis. The results indicate specific or coexpression patterns of rice bZIP transcription factors starting from floral transition to various stages of panicle and seed development. bZIP transcription factor-encoding genes in rice also displayed differential expression patterns in rice seedlings in response to abiotic stress and light irradiation. An effort has been made to link the structure and expression pattern of bZIP transcription factor-encoding genes in rice to their function, based on the information obtained from our analyses and earlier known results. This information will be important for functional characterization of bZIP transcription factors in rice.

show abstract

A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris)

Cited by 94 publications

References 29 publications

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

Transcriptome Profiling of Dehydration Stress in the Chinese Cabbage (Brassica rapa L. ssp. pekinensis) by Tag Sequencing

RFLP analysis of mitochondrial DNA in two cytoplasmic male sterility systems (CMS-D2 and CMS-D8) of cotton

Genomic Survey and Gene Expression Analysis of the Basic Leucine Zipper Transcription Factor Family in Rice

Contact Info

Product

Resources

About