A root acyl carrier protein-II from spinach is also expressed in leaves and seeds

Schmid, Katherine M.; Ohlrogge, John B.

doi:10.1007/bf00016126

Cited by 27 publications

(24 citation statements)

References 52 publications

(67 reference statements)

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“…4) clearly identifies the acpA gene product as an acyl carrier protein, a key cofactor in the synthesis and metabolism of fatty acids (39). Our finding of the acpA gene in the plastid genome of Cryptomonas '1 thus identifies lipid biosynthesis as a metabolic pathway involving plastid-encoded enzymes/proteins.…”

Section: Discussionmentioning

confidence: 75%

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The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Wang

Liu

1991

Proc. Natl. Acad. Sci. U.S.A.

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The plastid genome of Cryptomonas 4), a cryptomonad alga, contains three genes that have not previously been found in any organellar genome. Each of these genes encodes a functional class of organellar gene product not previously reported. The first gene, dnaK, encodes a polypeptide of the hsp7O heat shock protein family. The predicted amino acid sequence of the DnaK protein is-54% identical to that of the Escherichia coli hsp70 protein (DnaK), 50-53% identical to that of two nucleus-encoded mitochondrial hsp70 proteins, and 43-46% identical to that of several eukaryotic cytoplasmic members of the hsp7O protein family. The second gene, hipA, encodes a polypeptide resembling bacterial histonelike proteins. The predicted amino acid sequence of the HlpA protein is 25-53% identical to that of several bacterial histonelike proteins, and the identity increases to 39-76% over a conserved region corresponding to the Iong arm that binds DNA. The third gene, acpA, encodes an acyl carrier protein, which is a key cofactor in the synthesis and metabolism of fatty acids. Its predicted amino acid sequence is 36-59% identical to that ofeubacterial and plant chloroplast (nucleus-encoded) acyl carrier proteins.

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Section: Discussionmentioning

confidence: 75%

“…2. (39), Arabidopsis thaliana (A. t.) (40), and Brassica campestris (B. c.) (41). Symbols are as in Fig.…”

Section: Discussionmentioning

confidence: 99%

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Wang

Liu

1991

Proc. Natl. Acad. Sci. U.S.A.

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“…Arabidopsis ACP-2 mRNA Is Expressed in Roots, Leaves, and Developing Seeds Detailed analysis ofthe expression patterns ofACP isoforms in spinach has indicated the presence of both tissue-specific and constitutive ACPs (24,31). To examine Arabidopsis ACP isoform expression, we investigated the expression of ACP-2 at the mRNA level.…”

Section: Structure Of Two Acp Clones From Arabidopsismentioning

confidence: 99%

“…ACP isoforms with different patterns of tissue-specific expression have been characterized from spinach and barley (13,24). In spinach, a major isoform (ACP-I) is expressed only in leaves, whereas ACP-II, a minor isoform in leaves, is apparently "constitutive" as it is also found in roots and developing seeds (21,31). ACP cDNA clones have been isolated from spinach leaves (30) and roots (31), barley leaves (13), and Brassica campestris (28) and B. napus (29) developing seeds.…”

mentioning

confidence: 99%

“…In spinach, a major isoform (ACP-I) is expressed only in leaves, whereas ACP-II, a minor isoform in leaves, is apparently "constitutive" as it is also found in roots and developing seeds (21,31). ACP cDNA clones have been isolated from spinach leaves (30) and roots (31), barley leaves (13), and Brassica campestris (28) and B. napus (29) developing seeds. In addition, ACP genomic clones have been characterized from Arabidopsis (18,25), B. napus (32), and barley (14).…”

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confidence: 99%

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Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

Hloušek-Radojčić¹,

Post-Beittenmiller²,

Ohlrogge³

1992

Plant Physiol.

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We have characterized the occurrence and expression of multiple acyl carrier protein (ACP) isoforms in Arabidopsis thaliana (L.) Heynh ecotype Columbia. Immunoblot analysis of ACPs from Arabidopsis tissues separated by native polyacrylamide gel electrophoresis and I molar urea polyacrylamide gel electrophoresis revealed a complex pattem of multiple ACP isoforms. All tissues examined (leaves, roots, and seeds) expressed at least three forms of ACP. The immunoblot identifications of ACP bands were confirmed by acylation of ACP extracts with Escherichia coli acyl-ACP synthetase. A full-length cDNA clone has been isolated that has 70% identity with a previously characterized Arabidopsis genomic ACP clone (ACP-1) (MA Post-Beittenmiller, A HlousekRadojcic, JB Ohirogge [1989] Nucleic Acids Res 17: 1777). Based on RNA blot analysis, the cDNA clone represents an ACP that is expressed in leaves, seeds, and roots. In order to identify the protein products of each known ACP gene, their mature coding sequences have been expressed in E. coli. Using polymerase chain reactions, exons 11 and IlIl of the genomic ACP-1 clone and the mature coding sequences of the ACP-2 cDNA clone were subcloned into E. coil expression vectors. Site-directed mutagenesis was used to convert the amino acid sequence of the ACP-2 cDNA clone to that of the A2 clone of Lamppa and Jacks ([1991] Plant Mol Biol 16: 469-474), ACP-3. The three E. coli-expressed proteins have different mobilities on polyacrylamide gel electrophoresis gels and each comigrates with a different Arabidopsis ACP isoform expressed in leaves, seeds, and roots. Thus, all of the three cloned ACPs appear to be constitutively expressed Arabidopsis ACPs. In addition to these three ACP isoforms, protein blots indicate that seed, leaf, and root each express one or more tissue-specific isoforms.

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Lipid Metabolism

Schmid

2004

Handbook of Plant Biotechnology

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Lipids are an immensely diverse group of compounds, technically including all biomolecules that dissolve more readily in non‐polar solvents than in water. This review will emphasisze two prominent groups of lipids: the acyl lipids, whose hydrophobic units are derived from fatty acids, and the isoprenoids, made up of five‐carbon isopentene units. Acyl lipids, in the form of the triacylglycerols stored as energy reserves, underpin the vegetable oil industry. In addition, acyl lipids that dominate the bilayers of plant membranes, are indispensable for the protective layers that prevent plant desiccation and pathogen attack, and include several signalling molecules. Isoprenoids are even more diverse than acyl lipids, with more than 25,000known. Two prominent groups are the sterols, which are important membrane constituents, and the carotenoids, which collect light for photosynthesis, protect plants from excessive light, attract pollinators and fruit dispersal agents, and provide vitamin A precursors for consumers. Biotechnology has provided both a tool for understanding plant lipid metabolism, and a means of altering quality and quantity of these constituents to better serve the needs of industry and human nutrition better.

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A root acyl carrier protein-II from spinach is also expressed in leaves and seeds

Cited by 27 publications

References 52 publications

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

Lipid Metabolism

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