A Rolling-Circle Plasmid from Psychrobacter sp. TA144: Evidence for a Novel Rep Subfamily

Tutino, Maria Luisa; Duilio, Angela; Moretti, Maria Angela; Sannia, Giovanni; Marino, Gennaro

doi:10.1006/bbrc.2000.3148

Cited by 24 publications

(26 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(3) are reported the results obtained from the statistical analysis of bacterial sequences belonging to the HSLlike family. A similar analysis was reported by Manco and co-workers [41], analysing only 4 heterogeneous sequences: Psychrobacter TA144 lipase 2 (previously called Moraxella TA144; bacterial, psychrophiles) [43]; Acinetobacter calcoaceticus esterase 2 (bacterial, mesophiles); human Hormone-Sensitive Lipase (eukaryotic, mesophiles) and EST2 from A. acidocaldarius (bacterial, thermophiles). Manco and co-workers pointed out the role of the Cys and Asn contents Fig.…”

Section: Statistical Resultssupporting

confidence: 68%

An Overview on Thermal Adaptation of Esterases and Lipases Belonging to the HSL Family: New Insight on the Computational Analysis

Mandrich¹,

Pascale²

2011

curr chem biol

View full text Add to dashboard Cite

The mechanism used by proteins to maintain their thermostability throughout a wide range of temperature has been extensively investigated. Different aspects have been reported which explain protein thermal stability such as protein flexibility, loops length, number of charged residues, hydrophobic and ionic interactions, electrostatic interactions and their pathways, number and dimension of internal cavities. All these features have an effect on the protein global structure, but they are not the unique mechanism which explains the protein thermal stability.The molecular mechanisms of adaptation to the environment of an organism are reflected on different levels, with regards to DNA, genes and proteins expression, which are intrinsically adapted to the particular physical/chemical state.Amino acid composition is strictly related to environmental adaptation and, in this review, we present an up to date overview on thermal adaptation of esterases and lipases belonging to the HSL family. In particular, we discuss results obtained by different analyses on these enzymes and we re-analyze them from a statistical standpoint.

show abstract

Section: Statistical Resultssupporting

confidence: 68%

An Overview on Thermal Adaptation of Esterases and Lipases Belonging to the HSL Family: New Insight on the Computational Analysis

Mandrich¹,

Pascale²

2011

curr chem biol

View full text Add to dashboard Cite

show abstract

“…The largest replicons, pRWF101 (13.9 kb) and plasmid 1 of P. cryohalolentis K5 (41.2 kb), contain 14 and 44 open reading frames (ORFs), respectively, while the other plasmids are small (1.3–2.1 kb) cryptic replicons, encoding one or two ORFs. An in-depth sequence analysis has only been reported in the case of the plasmids harbored by the strain TA144 (Tutino et al 2000; Duilio et al 2001). …”

Section: Introductionmentioning

confidence: 99%

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

2012

View full text Add to dashboard Cite

The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slflocus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment.

show abstract

“…However, little is known about plasmids in psychrophiles. Since there is a considerable interest in the properties of cold adapted host-plasmid expression systems for the overproduction of heat-sensitive proteins (Tutino et al, 2000), the presence of plasmids in the novel isolates may be significant in recombinant DNA technology, particularly as a cloning vehicle for foreign genes.…”

mentioning

confidence: 99%

Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen

Groudieva

Grote

Antranikian

2003

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Using starch as a carbon source at a cultivation temperature of 4˚C, a number of Gram-negative, aerobic strains was isolated from sea-ice and sea-water samples collected at Spitzbergen in the Arctic. Analysis of the genetic diversity of the novel isolates by random amplification of polymorphic DNA (RAPD) and ERIC fingerprinting revealed a homogenic group of biofilm-forming bacteria that contained small extrachromosomal elements. As a representative of the group, strain Pull 5.3 T ,isolated from a sea-water sample, was used for detailed characterization. In recent years, increasing attention in research has been directed to cold-adapted micro-organisms that are able to grow at or close to the freezing point of water, namely psychrophiles. They are defined by an optimal temperature for growth of about 15˚C or below, a maximal growth temperature of about 20˚C and the ability to grow at 0˚C (Morita, 1975). In comparison, psychrotolerant microorganisms, sometimes also referred to as 'psychrotrophs', generally have optimum and maximum growth temperatures of 20˚C or above (Ingraham & Stokes, 1959).Cold-adapted micro-organisms are found in both permanently and temporarily cold habitats, which comprise more than 80 % of the Earth's biosphere. Oceans, covering threequarters of the Earth, polar regions (14 % of the Earth's surface), high mountains and deep lakes provide various aquatic and terrestrial cold environments where the temperature seldom or never reaches 5˚C (Gounot, 1999). The ability of micro-organisms to grow at low temperatures is not restricted to prokaryotes. A wide variety of micro-organisms, including bacteria, archaea, yeast, fungi and algae, is found in cold environments. These microorganisms are free-living in soil and fresh and saline waters or are associated with plants and cold-blooded animals such as fish or crustaceans. Among the bacteria, almost all types have been identified either after isolation (Ravenschlag et al., 1999; Bowman et al., 1997) or by detection in their natural habitats using a 16S rRNA approach (Fuhrman et al., 1993;DeLong et al., 1994;Vetriani et al., 1998). Unlike hyperthermophiles, they do not belong to new phyla. The majority of psychrophiles studied to date belong to the Gram-negative Proteobacteria. This is not surprising, since Gram-negatives are predominant in marine waters, where most investigations have been performed.Psychrophiles and psychrotolerant micro-organisms have a wide range of adaptations, including alterations in the protein and lipids of their membrane, energy-generation systems, protein synthesis and hydrolytic enzymes (Russell, 1998). Higher specific activity at low temperatures and thermosensitivity of cold-active enzymes provide a valuable source for exploration of novel biotechnological processes (Feller & Gerday, 1997). Since it has become clear that psychrophiles also represent a naturally occurring model for investigating protein adaptation to cold (Aghajari et al., 1998), an increasing number of novel bacterial strains from different Arctic and A...

show abstract

A Rolling-Circle Plasmid from Psychrobacter sp. TA144: Evidence for a Novel Rep Subfamily

Cited by 24 publications

References 24 publications

An Overview on Thermal Adaptation of Esterases and Lipases Belonging to the HSL Family: New Insight on the Computational Analysis

An Overview on Thermal Adaptation of Esterases and Lipases Belonging to the HSL Family: New Insight on the Computational Analysis

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen

Contact Info

Product

Resources

About