A Role of VAMP8/Endobrevin in Regulated Exocytosis of Pancreatic Acinar Cells

Wang, Cheng-Chun; Ng, Chee Peng; Lü, Lei; Atlashkin, Vadim; Zhang, Wei; Seet, Li‐Fong; Hong, Wanjin

doi:10.1016/j.devcel.2004.08.002

Cited by 162 publications

(254 citation statements)

References 51 publications

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“…6; and data not shown). Western blotting of the Percoll gradient fractions for the secretory granule SNARE Vamp8 (Wang et al, 2004;Weng et al, 2007) confirmed that secretory granules were enriched in fraction 3 of the Percoll gradient. We also detected some Vamp8 in lighter fractions toward the top of the Percoll gradient.…”

Section: Sdmg1 Is a Component Of Pancreatic Secretory Granulesmentioning

confidence: 72%

“…The expression of Sdmg1 in the exocrine secretory cells in the pancreas, salivary gland, and mammary gland, and the localization of Sdmg1 to pancreatic secretory granules, bears some resemblance to the expression and subcellular localization of the Vamp8 SNARE (Wang et al, 2004;Weng et al, 2007). Expression of Vamp8 and Sdmg1 are also both up-regulated in a male-specific manner during embryonic gonad development (Best et al, 2008).…”

Section: Discussionmentioning

confidence: 97%

“…Sdmg1 in each of these secretory exocrine cells bears some resemblance to the subcellular localization of Vamp8, a SNARE associated with secretory granules in these cell types (Wang et al, 2004(Wang et al, , 2007.…”

Section: A-imentioning

confidence: 92%

“…During secretory granule exocytosis, there appears to be little diffusion from the secretory granule membrane into the plasma membrane and, once the secretory granule cargo is expelled, the membrane of the secretory granule remains distinct from the plasma membrane and is eventually endocytosed and recycled (Thorn et al, 2004). Around 80 -90% of all protein secretion in the secretory exocrine cells of the pancreas and parotid salivary gland occurs through secretory granule exocytosis (Van Nest et al, 1980;von Zastrow and Castle, 1987); therefore, proteins involved in the regulated secretory pathway are expressed at high levels in these secretory exocrine cells (Wang et al, 2004(Wang et al, , 2007.…”

Section: Introductionmentioning

confidence: 99%

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Sdmg1 is a component of secretory granules in mouse secretory exocrine tissues

Best

Adams

2008

Developmental Dynamics

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Sdmg1 is a conserved eukaryotic transmembrane protein that is mainly expressed in the gonads where it may have a role in mediating signaling between somatic cells and germ cells. In this study we demonstrate that secretory exocrine cells in the pancreas, salivary gland, and mammary gland also express Sdmg1. Furthermore, we show that Sdmg1 expression is up-regulated during pancreas development when regulated secretory granules start to appear, and that Sdmg1 colocalizes with secretory granule markers in adult pancreatic acinar cells. In addition, we show that Sdmg1 co-purifies with secretory granules during subcellular fractionation of the pancreas and that Sdmg1 and the secretory granule marker Vamp2 are localized to distinct subdomains in the secretory granule membrane. These data suggest that Sdmg1 is a component of regulated secretory granules in exocrine secretory cells and that the developmental regulation of Sdmg1 expression is related to a role for Sdmg1 in post-Golgi membrane trafficking.

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Section: Sdmg1 Is a Component Of Pancreatic Secretory Granulesmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 97%

Section: A-imentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Sdmg1 is a component of secretory granules in mouse secretory exocrine tissues

Best

Adams

2008

Developmental Dynamics

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“…Regarding the secretory signaling pathway and machinery, CCKAR (Fig. 5I), VAMP8 -a SNARE protein associated with ZG whose function is crucial for secretion (36) (Fig. 5G), and syntaxin 4 -a membrane protein of pancreatic acinar cells (36) - (Fig.…”

Section: Cells Having Activated the Acinar Gene Expression Program DImentioning

confidence: 99%

Murine Embryonic Stem Cell–Derived Pancreatic Acinar Cells Recapitulate Features of Early Pancreatic Differentiation

et al. 2008

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Background & Aims-Acinar cells constitute 90% of the pancreas epithelium, are polarized, and secrete digestive enzymes. These cells play a crucial role in pancreatitis and pancreatic cancer. However, there are no models to study normal acinar cell differentiation in vitro. The aim of this work was to generate and characterize purified populations of pancreatic acinar cells from embryonic stem cells (ES).

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Toxicology of the Exocrine Pancreas

Foster¹

2009

General, Applied and Systems Toxicology

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The exocrine pancreas is not a common target organ for toxic drugs and chemicals. It is however the fifth most common human cancer in the western world, and acute and chronic pancreatitis, through various identifiable and unidentified causes, is a common disease in both the developed and developing countries of the world. Both inflammatory disease and cancer of the pancreas is correlated to lifestyle with poor diet and habits, such as moderate to high alcohol intake and smoking, having positive correlations to developing the disease. The chapter describes the functional anatomy of the organ together with its biochemistry that determines toxicity, in particular the role of oxidant damage in the development of cell and tissue damage is thought to be important. The consequences of toxic damage to the acinar cells are severe and primarily result from the auto‐activation and hence autodigestion of the tissue by the digestive enzymes normally contained within the exocrine cells, a cascade of apoptosis/necrosis and fibrosis with the ultimate collapse of the organ, subsequent shock, multi‐organ collapse and death. The organ has complex molecular mechanisms to prevent premature activation of the digestive enzymes and also has defence mechanisms present to neutralise any premature enzyme activation but these can be rendered ineffectual by factors such as alcohol abuse and a poor diet. Chemical exposure, through both occupational and consumer product usage, has been associated with the development of both pancreatic neoplastic and non‐neoplastic disease but the relationship between exposure and disease has never developed further than a simple association and it is likely that the development of pancreatic disease is a multi‐faceted combination of inter‐related events, none of which in isolation can induce disease. The pancreas has its own array of phase 1 and 2 metabolic enzyme systems and the role of pancreatic drug and chemical activation and detoxification in inducing toxicity is discussed together with the possible secondary effects of hepatic drug/chemical activation and detoxification. Finally the role of animal models of pancreatic disease is discussed in terms of their contribution to understanding the aetiology of the human conditions and the possible use of therapeutic intervention in treating the disease.

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A Role of VAMP8/Endobrevin in Regulated Exocytosis of Pancreatic Acinar Cells

Cited by 162 publications

References 51 publications

Sdmg1 is a component of secretory granules in mouse secretory exocrine tissues

Sdmg1 is a component of secretory granules in mouse secretory exocrine tissues

Murine Embryonic Stem Cell–Derived Pancreatic Acinar Cells Recapitulate Features of Early Pancreatic Differentiation

Toxicology of the Exocrine Pancreas

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