A role of the cancer cell membrane fluidity in the cancer metastases: An ESR study.

Abstract

AH66F or Yoshida sarcoma (YS) cells were transplanted intraperitoneally into male Donryu rats. Cancer cells obtained from ascites were suspended in saline solution (10' cells ml) after washing. Then, 0.1 ml of each suspension obtained from both strains was injected into the tail vein of 5 rats, respectively. Each metastatic nodule, 1 mm or less in a diameter, thus obtained was then injected into the peritoneal cavity in which these metastatic cells come to free. After 10 days, cancer cells obtained from each ascites were suspended in phosphate buffered saline (Ca2+ and Mg2+ free, pH 7.2) after washing. Each suspension (10' cells/ml) was violently vibrated with a definite amount of 5-doxyl stearic acid and spin labeling of cancer cell membrane was done. Furthermore, each specimen thus obtained was subjected to the electron spin resonance (ESR) measurement and the order parameter was determined from the spectra. In both YS and AH66F strains, the cell membrane fluidity of the metastatic cancer cell was increased at each temperature measured from 5°C through 35°C. The results obtained here suggest that the change of the cell membrane fluidity of cancer cell is closely related with the cancer metastases.cancer ; cell membrane fluidity ; metastases ; ESR ; phospholipid Metastasis to other organ is one of the characteristics of cancer which makes it difficult to cure cancer patients. Therefore, it is desirable to clarify the mechanism of cancer metastasis in order to cure cancer patients. Fidler (1973a) reported that only 1% of B16 melanoma cells injected intravenously had survived to form actual pulmonary metastasis. Fidler (1973b) also succeeded in obtaining the highly metastatic strains of B16 melanoma by means of intravenous injection and tissue culture of the metastatic lesions. Bosmann et al. (1973) noticed the biochemical features of the most highly metastatic strain (F10), that is, the change in the electrophoretic mobility, surface glycoprotein, proteases and increasing degradative enzyme as compared with those of low metastatic B16 melanoma strain (F 1). Miller et al. (1985) suggested that metastasis of human colonic cancer

Epithelial-Stromal Interactions in Human Breast Cancer: Effects on Adhesion, Plasma Membrane Fluidity and Migration Speed and Directness

“…Even though no univocal trend is evident regarding the direction of alterations in tumor cell membrane fluidity [54], in most tumors malignant cell membranes have been found to possess higher fluidity than those of their healthy counterparts. It has also been shown that such structural change significantly correlates with an increased migratory/invasive aptitude and malignant potential of tumor cells as well as with a poor prognosis [52], [53], [55], [56]. Therefore, in the present study, two-photon microscopy imaging of cells labelled with the membrane fluorescent probe Laurdan was used to investigate whether fluidity changes in plasma membranes could occur as a result of tumor cell/fibroblast co-culturing.…”

“…Changes in the motional freedom of lipids and protein molecules in the cellular membrane represent a critical feature of pathological cells, influencing several aspects of their behaviour such as the response to chemotherapeutic agents [48], [49], availability or activation of membrane receptors [50], [51], migratory, invasive and/or metastatic potential [52], [53]. Even though no univocal trend is evident regarding the direction of alterations in tumor cell membrane fluidity [54], in most tumors malignant cell membranes have been found to possess higher fluidity than those of their healthy counterparts.…”

Biophysics in cancer: The relevance of drug-membrane interaction studies

“…In fact, alterations in membrane fluidity in cancer cells may be correlated with their malignant potential [30,32] and capability to metastasize [33]. Although no pattern has been described, such alterations can be responsible for cancer cell sensitivity or resistance to several anticancer drugs [31].…”

Screening of FOXD3 targets in lung cancer via bioinformatics analysis

“…Plasma membrane fluidity depends on the composition of the lipids and proteins in the membrane, and has been demonstrated to be significantly associated with the malignant potential of cancer cells (28), with alterations in the plasma membrane fluidity of cancer cells associated with their capacity to form metastases (29). In lung cancer, studies reported that patients with high plasma membrane fluidity had poorer prognoses than those with less fluid membranes, and the fluidity variable may be used as an independent additional prognostic factor (28,30,31).…”