A role of flavonoids in cytochrome c-cardiolipin interactions

Rice, Malaysha; Wong, B.; Oja, Mare; Samuels, Kelley; Williams, Alicia K.; Fong, Jim C.; Sapse, Anne‐Marie; Maran, Uko; Korobkova, Ekaterina A.

doi:10.1016/j.bmc.2021.116043

Cited by 5 publications

(2 citation statements)

References 59 publications

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“…Nitric oxide is a well-known inhibitor of cyt c peroxidase activity and thus may downregulate apoptosis [104]. The ROS scavenger activity of antioxidant molecules, such as flavonoids, may prevent cellular aging and they can inhibit cyt c peroxidase activity, preventing proapoptotic events [105].…”

Section: Cytochrome C-cardiolipin Interaction In Neurodegeneration An...mentioning

confidence: 99%

Cytochrome c Interaction with Cardiolipin Plays a Key Role in Cell Apoptosis: Implications for Human Diseases

et al. 2022

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In the cell cytochrome, c performs different functions depending on the environment in which it acts; therefore, it has been classified as a multifunction protein. When anchored to the outer side of the inner mitochondrial membrane, native cytochrome c acts as a Schweitzer-StennerSchweitzer-Stenner that transfers electrons from cytochrome c reductase to cytochrome c oxidase in the respiratory chain. On the other hand, to interact with cardiolipin (one of the phospholipids making up the mitochondrial membrane) and form the cytochrome c/cardiolipin complex in the apoptotic process, the protein reorganizes its structure into a non-native state characterized by different asymmetry. The formation of the cytochrome c/cardiolipin complex is a fundamental step of the apoptotic pathway, since the structural rearrangement induces peroxidase activity in cytochrome c, the subsequent permeabilization of the membrane, and the release of the free protein into the cytoplasm, where cytochrome c activates the apoptotic process. Apoptosis is closely related to the pathogenesis of neoplastic, neurodegenerative and cardiovascular diseases; in this contest, the biosynthesis and remodeling of cardiolipin are crucial for the regulation of the apoptotic process. Since the role of cytochrome c as a promoter of apoptosis strictly depends on the non-native conformation(s) that the protein acquires when bound to the cardiolipin and such event leads to cytochrome c traslocation into the cytosol, the structural and functional properties of the cytochrome c/cardiolipin complex in cell fate will be the focus of the present review.

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Section: Cytochrome C-cardiolipin Interaction In Neurodegeneration An...mentioning

confidence: 99%

Cytochrome c Interaction with Cardiolipin Plays a Key Role in Cell Apoptosis: Implications for Human Diseases

et al. 2022

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“…Indeed, Cyt c –CL nanoparticles sharply activated apoptosis in two A2780 human ovarian cancer cell lines, including those resistant to doxorubicin [ 3 ]. On the other hand, strategies for protection against apoptosis are developed using, e.g., flavonoids that affect the interaction of cytochrome c with cardiolipin [ 4 ]. Thus, the mechanism of interaction between cytochrome c and cardiolipin is being actively studied to control this critical process.…”

Section: Introductionmentioning

confidence: 99%

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

et al. 2023

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Thermal lens spectrometry along with spectrophotometric titration were used to assess the composition of the complex of oxidized cytochrome c (ferricytochrome c) with 1,1′,2,2′-tetraoleyl cardiolipin, which plays a key role in the initiation of apoptosis. Spectrophotometric titration was carried out for micromolar concentrations at which the complex is mainly insoluble, to assess the residual concentration in the solution and to estimate the solubility of the complex. Thermal lens spectrometry was used as a method of molecular absorption spectroscopy, which has two advantages over conventional optical transmission spectroscopy: the higher sensitivity of absorbance measurements and the possibility of studying the light absorption by chromophores and heat transfer in complex systems, such as living cells or tissues. Thermal lens measurements were carried out at nanomolar concentrations, where the complex is mainly in solution, i.e., under the conditions of its direct measurements. From the thermal lens measurements, the ratios of cytochrome c and cardiolipin in the complex were 50 at pH 7.4; 30 at pH 6.8; and 10 at pH 5.5, which fit well to the spectrophotometric data. The molecular solubility of the complex at pH 6.8–7.4 was estimated as 30 µmol/L.

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Online ligand screening for cytochrome C from herbal medicines through “four-in-one” measurement

Li,

Zhang,

Gong

et al. 2024

Pharmacological Research

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A role of flavonoids in cytochrome c-cardiolipin interactions

Cited by 5 publications

References 59 publications

Cytochrome c Interaction with Cardiolipin Plays a Key Role in Cell Apoptosis: Implications for Human Diseases

Cytochrome c Interaction with Cardiolipin Plays a Key Role in Cell Apoptosis: Implications for Human Diseases

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

Online ligand screening for cytochrome C from herbal medicines through “four-in-one” measurement

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