A role of alpha-tocopherol and phylloquinone in the modulation of uterine contractility and reproductive function in mouse models

Bafor, Enitome E.; Ebidame, Victory O.; Elvis-Offiah, Uloma B.; Omoruyi, Osemelomen; Eze, Gerald Ikechi; Igbinuwen, Osamwonyi; Braimoh, Kadiri P.

doi:10.1016/j.medici.2017.05.002

Cited by 11 publications

(6 citation statements)

References 46 publications

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“…It would be interesting to find out whether these processes also occur in UFs. This has not been studied yet, but these results could be also explained by estrogen-like properties of vitamin E. Bafor et al, in a very interesting study about the effects of AT on the concentrations of reproductive hormones in mice, found that treatment with AT significantly increased the plasma levels of luteinizing hormone (LH), estradiol, and progesterone [ 70 ]. They also found that AT affected not only the hypophysis alone (effect on LH), but also the ovaries (effect on estrogen and progesterone) [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Ciebiera

Szymańska-Majchrzak

Sentkowska

et al. 2018

BioMed Research International

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Uterine fibroids (UFs) are benign tumors of the reproductive tract, arising from smooth muscle cells of the uterus. Steroid hormones, estrogen, and progesterone are considered to be the most important links in the pathophysiology of UFs. Alpha-tocopherol (AT) is the most active form of vitamin E. What is important as far as UFs are concerned is that ATs contain structural determinants, which makes them possible ligands for estrogen receptors (ERs). We present a retrospective cohort study performed in a university teaching hospital. We included a total of 162 patients divided into 2 groups: with UFs and controls. The effects of age, body mass index (BMI), positive medical history, parity, and AT serum concentrations on the risk for the development of UFs were investigated. Mean AT serum concentrations were 11.66 ± 4.97 μg/ml and 7.83 ± 3.13 μg/ml (medians 10.56 μg/ml and 7.42 μg/ml) in patients with UFs confirmed on ultrasound and controls, respectively. The presented difference was statistically significant. Higher BMI, positive family history, and low parity were found to be major risk factors for UFs. In our study, we confirmed that elevated serum AT concentration might be an important risk factor for UFs in Caucasian women. Further research in this area is necessary.

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Section: Discussionmentioning

confidence: 99%

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Ciebiera

Szymańska-Majchrzak

Sentkowska

et al. 2018

BioMed Research International

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“…Each concentration was allowed a contact time of 5 min. (Bafor et al, 2017a). This procedure was performed for both non-pregnant and pregnant mouse uteri.…”

Section: Experiments On Spontaneous Uterine Contractilitymentioning

confidence: 99%

Tocolytic activity assessment of the methanol leaf extract of Justicia flava Vahl (Acanthaceae) on mouse myometrial contractility and preliminary mass spectrometric determination of secondary metabolites

Bafor

Ukpebor

Omoruyi

et al. 2019

Journal of Ethnopharmacology

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Ethnopharmacological relevance: The leaves of Justicia flava are traditionally used in the South of Nigeria to prevent preterm births. Aim of the study: In this study, the activity of the methanol leaf extract of J. flava (JF) was investigated on uterine contractility in non-pregnant and pregnant isolated mouse tissues. Material and methods: The effects on spontaneous, oxytocin, and KCl-induced contractions were determined. The effects in calcium-free media were also determined. Possible mechanisms of activity were investigated using receptor and channel modulators. Mass spectrometric analysis was additionally performed on the leaf extract to identify secondary metabolites. Results: JF was observed to inhibit spontaneous, oxytocin and high KCl-induced uterine contractility. JF also inhibited contractions in Ca 2+free media. JF was found to exert its inhibitory effect via interaction with inositol triphosphate and ryanodine receptors and also through modulation of K +-channels. Lignans and alkaloids were identified with the lignans being the most abundant in JF. Conclusion: JF has been shown to potently inhibit uterine contractions in non-pregnant and pregnant isolated mouse uterus. The inhibitory activity of JF has been shown to occur via blockade of extracellular and intracellular calcium entry and these effects may be due to the lignans identified in the JF. JF has therefore been shown in this study to be a lead plant in the discovery of new drugs with uterine inhibitory activity.

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“…A contact time of 3 min was allowed following each concentration of http://www.jomped.co.za Open Access extract administered. After each set of administration, the tissues were washed three times and a washout period minimum of 10 min was allowed for the tissue to recover before the next administration (Bafor et al 2017).…”

Section: Experiments On the Effect Of Extract On Spontaneous Uterine Contractionmentioning

confidence: 99%

Modulation of ex-vivo uterine contraction by the methanol leaf extract of Alchornea laxiflora Benth. (Euphorbiaceae) and preliminary spectrometric identification of associated secondary metabolites

Bafor

Nwogu

Elvis-Offiah

et al. 2018

JOMPED

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Background: The leaves of Alchornea laxiflora are traditionally used in the south of Nigeria to prevent preterm births. Aim: This study was designed to investigate the activity of A. laxiflora on uterine contractility. Setting: The leaves of the plant were collected from forests in Egor, Benin City, Nigeria. Methods: The leaves were cleaned and extracted in methanol. The extract (0.005 mg/mL–3.5 mg/mL) was tested on spontaneous uterine contraction and on oxytocin-induced contraction in normal and Ca2+-free media. The plant extract (0.0035 mg/mL, 0.035 mg/mL, 0.35 mg/mL and 3.5 mg/mL) was tested on high KCl-induced uterine contractions (80 mM). The plant extract (3.5 mg/mL) was also studied in the presence of amiodarone and glibenclamide in separate experiments. Mass spectrometric analysis was additionally performed on the plant extract in order to identify significant secondary metabolites that may have contributed to the activity of the plant. Results: The plant extract inhibited spontaneous, oxytocin and high KCl-induced uterine contractions and also significantly inhibited (p < 0.01) oxytocin-induced uterine contraction in Ca2+-free medium. The plant extract significantly inhibited (p < 0.01 and p < 0.05) oxytocin’s amplitude in the presence of amiodarone and glibenclamide, respectively. Secondary metabolites belonging to classes of fatty acids, glycols, terpenes, flavonoid glycosides and porphyrins were identified. Conclusion: Alchornea laxiflora inhibited mouse uterine contractility possibly through interaction with potassium and calcium channels. Of the known metabolites identified, 3-deoxy-arabino-hept-2-ulosonic acid, 17-hydroxyingenol and phaeophorbide-a methyl inhibit uterine contractility and may contribute to the activity of A. laxiflora in utero.

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A role of alpha-tocopherol and phylloquinone in the modulation of uterine contractility and reproductive function in mouse models

Abstract: Alpha-tocopherol and phylloquinone have been shown to directly modulate uterine contractility and reproductive function and may contribute to the management and treatment of reproductive disorders.

Cited by 11 publications

References 46 publications

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Tocolytic activity assessment of the methanol leaf extract of Justicia flava Vahl (Acanthaceae) on mouse myometrial contractility and preliminary mass spectrometric determination of secondary metabolites

Modulation of ex-vivo uterine contraction by the methanol leaf extract of Alchornea laxiflora Benth. (Euphorbiaceae) and preliminary spectrometric identification of associated secondary metabolites

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