A Role for αvβ3 Integrin During Implantation in the Rabbit Model1

Illera, M.; Lorenzo, P.L.; Gui, Yaoting; Beyler, Stanley A; Apparao, K.B.C.; Lessey, Bruce A.

doi:10.1093/biolreprod/68.3.766

Cited by 92 publications

(55 citation statements)

References 46 publications

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“…Function-inhibiting antibodies that target integrin αvβ3 or αvβ8 -both of which are expressed on trophectoderm as well as on receptive phase epithelium -RGD peptide and knockdown of epithelial αvβ3 can reduce embryo attachment in vitro (Kaneko et al, 2011(Kaneko et al, , 20132014a) and in vivo (Illera et al, 2000(Illera et al, , 2003Kumar et al, 2015). This implies an interaction between integrin αv and an extracellular bridging ligand (see poster) regarding attachment (Singh and Aplin, 2009).…”

Section: Box 2: Demonstrating Integrin Function In Implantationmentioning

confidence: 99%

Embryo–epithelium interactions during implantation at a glance

Aplin

Ruane

2017

Journal of Cell Science

191

140

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At implantation, with the acquisition of a receptive phenotype in the uterine epithelium, an initial tenuous attachment of embryonic trophectoderm initiates reorganisation of epithelial polarity to enable stable embryo attachment and the differentiation of invasive trophoblasts. In this Cell Science at a Glance article, we describe cellular and molecular events during the epithelial phase of implantation in rodent, drawing on morphological studies both in vivo and in vitro, and genetic models. Evidence is emerging for a repertoire of transcription factors downstream of the master steroidal regulators estrogen and progesterone that coordinate alterations in epithelial polarity, delivery of signals to the stroma and epithelial cell death or displacement. We discuss what is known of the cell interactions that occur during implantation, before considering specific adhesion molecules. We compare the rodent data with our much more limited knowledge of the human system, where direct mechanistic evidence is hard to obtain. In the accompanying poster, we represent the embryo-epithelium interactions in humans and laboratory rodents, highlighting similarities and differences, as well as depict some of the key cell biological events that enable interstitial implantation to occur.

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Section: Box 2: Demonstrating Integrin Function In Implantationmentioning

confidence: 99%

Embryo–epithelium interactions during implantation at a glance

Aplin

Ruane

2017

Journal of Cell Science

191

140

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“…Both constitutive and cyclical expression of integrins in the uterus has been observed, and they are now considered the most decisive criterion for determining uterine receptivity (Lessey et al 1992(Lessey et al , 1996. Although there are many integrin heterodimer pairs expressed in the human uterus, the apical localization of a v b 3 and a v b 5 integrins in the mouse, human, baboon, rabbit, pig, and sheep luminal epithelium, makes these specific integrin pairs appropriate candidates for mediating trophoblast/epithelial interactions (Bowen et al 1996, Lessey et al 1996, Fazleabas et al 1997, Burghardt et al 2002, Illera et al 2003. Furthermore, in the human baboon, and pig, integrin b 3 is temporally regulated, such that its expression coincides with the window of receptivity (Lessey et al 1992, Bowen et al 1996, Fazleabas et al 1997.…”

Section: Integrinsmentioning

confidence: 99%

Animal models of implantation

Lee

DeMayo²

2004

Reproduction

233

155

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Implantation is an intricately timed event necessary in the process of viviparous birth that allows mammals to nourish and protect their young during early development. Human implantation begins when the blastocyst both assumes a fixed position in the uterus and establishes a more intimate relationship with the endometrium. Due to the impracticalities of studying implantation in humans, animal models are necessary to decipher the molecular and mechanical events of this process. This review will discuss the differences in implantation between different animal models and describe how these differences can be utilized to investigate discrete implantation stages. In addition, factors that have been shown to be involved in implantation in the human and other various animal models including growth factors, cytokines, modulators of cell adhesion, and developmental factors will be discussed, and examples from each will be given.

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“…Development of focal adhesions in vivo at the maternal-conceptus interface during initial stages of implantation and placentation has not been reported, no doubt due in part to the complexity of isolating the initial attachment sites. However, treatments that block integrin attachment reduce the number of implantation sites in mice and rabbits (Illera et al 2000(Illera et al , 2003. Large focal adhesions along with abundant ECM have been detected later in pregnancy at the apical surfaces of LE and conceptus trophectoderm in interplacentomal attachment sites in sheep.…”

Section: Introductionmentioning

confidence: 99%

Comparative aspects of implantation

Bazer

Spencer²,

Johnson³

et al. 2009

Reproduction

345

285

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Uterine receptivity to implantation of blastocysts in mammals includes hatching from zona pellucida, precontact with uterine luminal (LE) and superficial glandular (sGE) epithelia and orientation of blastocyst, apposition between trophectoderm and uterine LE and sGE, adhesion of trophectoderm to uterine LE/sGE, and, in some species, limited or extensive invasion into the endometrial stroma and induction of decidualization of stromal cells. These peri-implantation events are prerequisites for pregnancy recognition signaling, implantation, and placentation required for fetal-placental growth and development through the remainder of pregnancy. Although there is a range of strategies for implantation in mammals, a common feature is the requirement for progesterone (P 4 ) to downregulate expression of its receptors in uterine epithelia and P 4 prior to implantation events. P 4 then mediates its effects via growth factors expressed by stromal cells in most species; however, uterine luminal epithelium may express a growth factor in response to P 4 and/or estrogens in species with a true epitheliochorial placenta. There is also compelling evidence that uterine receptivity to implantation involves temporal and cell-specific expression of interferon (IFN)-stimulated genes that may be induced directly by an IFN or induced by P 4 and stimulated by an IFN. These genes have many roles including nutrient transport, cellular remodeling, angiogenesis and relaxation of vascular tissues, cell proliferation and migration, establishment of an antiviral state, and protection of conceptus tissues from challenges by the maternal immune cells.

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A Role for αvβ3 Integrin During Implantation in the Rabbit Model1

Cited by 92 publications

References 46 publications

Embryo–epithelium interactions during implantation at a glance

Embryo–epithelium interactions during implantation at a glance

Animal models of implantation

Comparative aspects of implantation

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