A role for TNFα in glial activation, myelin degradation and lesion-induced axonal sprouting

Hegelund, I.V.; Jensen, Marina Bergen; Agger, Ralf; Owens, Trevor; Finsen, Bente

doi:10.1016/s0165-5728(98)91345-5

Cited by 2 publications

(1 citation statement)

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“…To date PP-lesioning of the adult rat hippocampus has been shown to lead to microglial synthesis of IL-1 [18], IGF-1 mRNA and protein [30], and TGFb1 mRNA [60], which at least in the SJL/J mouse is supplemented by the expression of TNF mRNA [33]. Astrocytes have in the rat been shown to synthesize increased levels of FGF-2 [29], and CNTF mRNA and protein [31].…”

Section: Collateral Axonal Sprouts Vs Activated Micro-and Astroglia Amentioning

confidence: 99%

Axonal sprouting regulates myelin basic protein gene expression in denervated mouse hippocampus

Jensen

Poulsen

Finsen

2000

Intl J of Devlp Neuroscience

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The regulation of oligodendrocyte gene expression and myelination in vivo in the normal and injured adult CNS is still poorly understood. We have analyzed the effects of axotomy-induced axonal sprouting and microglial activation, on oligodendrocyte myelin basic protein (MBP) gene expression from 2 to 35 days after transection of the entorhino-hippocampal perforant path axonal projection. In situ hybridization analysis showed that anterograde axonal and terminal degeneration lead to upregulated oligodendrocyte MBP mRNA expression starting between day 2 and day 4, in (1) the deep part of stratum radiatum of CA3 and the dentate hilus, which display axonal sprouting but no degenerative changes or microglial activation, and (2) the outer part of the molecular layer of the fascia dentata, and in stratum moleculare of CA3 and stratum lacunosum-moleculare of CA1, areas that display dense anterograde axonal and terminal degeneration, myelin degenerative changes, microglial activation and axotomi-induced axonal sprouting. Oligodendrocyte MBP mRNA expression reached maximum in both these areas at day 7. MBP gene transcription remained constant in stratum radiatum, stratum pyramidale and stratum oriens of CA1, areas that were unaffected by perforant path transection. These results provide strong evidence that oligodendrocyte MBP gene expression can be regulated by axonal sprouting independently of microglial activation in the injured adult CNS.

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