A role for the tet(O) plasmid in maintaining Campylobacter plasticity

Friis, Lorna M.; Pin, Carmen; Taylor, Diane E.; Pearson, Bruce M.; Wells, Jerry M.

doi:10.1016/j.plasmid.2006.06.005

Cited by 21 publications

(23 citation statements)

References 47 publications

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“…venerealis isolate 84-112 (V81) verified that this system has also retained conjugative functions (Kienesberger et al, unpublished). Consistent with that observation, the structure of the C. fetus genomic island is highly syntenic to resistance plasmids found in other Campylobacter species, pCC31 and pTet (8,26). Some T4SS components also mediate DNA uptake, thus conferring a natural competence on organisms such as C. jejuni or H. pylori (7,42).…”

Section: Discussionsupporting

confidence: 77%

“…Alternatively, the distally located orf29, encoding a putative lytic transglycosylase, may provide that function. The T4SS exhibits the most apparent synteny and homology with the resistance (R) plasmids pCC31 of C. coli and pTet of C. jejuni (8,26), as well as an uncharacterized locus within the Campylobacter hominis genome (GenBank accession no. NC_009714.1).…”

Section: Resultsmentioning

confidence: 99%

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A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

et al. 2010

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The pathogen Campylobacter fetus comprises two subspecies, C. fetus subsp. fetus and C. fetus subsp. venerealis. Although these taxa are highly related on the genome level, they are adapted to distinct hosts and tissues. C. fetus subsp. fetus infects a diversity of hosts, including humans, and colonizes the gastrointestinal tract. In contrast, C. fetus subsp. venerealis is largely restricted to the bovine genital tract, causing epidemic abortion in these animals. In light of their close genetic relatedness, the specific niche preferences make the C. fetus subspecies an ideal model system to investigate the molecular basis of host adaptation. In this study, a subtractive-hybridization approach was applied to the genomes of the subspecies to identify different genes potentially underlying this specificity. The comparison revealed a genomic island uniquely present in C. fetus subsp. venerealis that harbors several genes indicative of horizontal transfer and that encodes the core components necessary for bacterial type IV secretion. Macromolecular transporters of this type deliver effector molecules to host cells, thereby contributing to virulence in various pathogens. Mutational inactivation of the putative secretion system confirmed its involvement in the pathogenicity of C. fetus subsp. venerealis.

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Section: Discussionsupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

et al. 2010

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“…The T4SS of C. fetus ssp. venerealis exhibits the most apparent synteny and homology with the resistance plasmids pCC31 of C. coli and pTet of C. jejuni (Batchelor et al ., 2004; Friis et al ., 2007). As described in the previous section, this T4SS supports intra‐ and interspecies mobilization of plasmids based on pIP1455 (S. Kienesberger, G. Gorkiewicz, A. Fauster and E.L. Zechner, unpublished) and is therefore useful for disseminating plasmids from C. fetus ssp.…”

Section: Molecular Geneticsmentioning

confidence: 99%

New molecular microbiology approaches in the study of Campylobacter fetus

Kienesberger¹,

Gorkiewicz²,

Wolinski³

et al. 2010

Microbial Biotechnology

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SummaryCampylobacter fetus infection is a substantial problem in herds of domestic cattle worldwide and a rising threat in human disease. Application of comparative and functional genomics approaches will be essential to understand the molecular basis of this pathogen's interactions with various hosts. Here we report recent progress in genome analyses of C. fetus ssp. fetus and C. fetus ssp. venerealis, and the development of molecular tools to determine the genetic basis of niche‐specific adaptations. Campylobacter research has been strengthened by the rapid advancements in imaging technology occurring throughout microbiology. To move forward in understanding the mechanisms underlying C. fetus virulence, current efforts focus on developing suitable in vitro models to reflect host‐ and tissue‐specific aspects of infection.

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“…The pVir plasmid has been suggested to contain some genes of a potential type IV secretion system [13], but the contribution of the pVir plasmid to C. jejuni virulence is still under debate [14]. The pTet plasmid confers tetracycline resistance and contains genes encoding for a type IV secretion system, which is thought to function in conjugative transfer [15,16]. A plasmid found in C. coli, pCC31, is >90% identical to pTet.…”

Section: Plasmidsmentioning

confidence: 99%

“…C. jejuni and C. coli are capable of natural transformation, and lack several DNA repair mechanisms, which contribute to genome plasticity [5]. In addition, bacteriophages and plasmids are thought to contribute to genome plasticity and DNA rearrangements [16,32]. Several techniques have been employed for the analysis of genetic variability and for typing of C. jejuni and C. coli.…”

Section: Comparative Genomic Analysis Of Campylobactermentioning

confidence: 99%

Genomics of Thermophilic <i>Campylobacter</i> Species

Gaskin¹,

Reuter²,

Shearer³

et al. 2009

Microbial Pathogenomics

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The thermophilic Campylobacter species C. jejuni and C. coli are important human pathogens, which are major causes of bacterial gastroenteritis. The recent progress in genomics techniques has allowed for a rapid increase in our knowledge of the molecular biology of Campylobacter species, but needs to be matched by concurrent increases in our understanding of the unique biology of these organisms. Campylobacter species display significant levels of genomic variation via natural transformation, phase variation, plasmid transfer and infection with bacteriophages, and this poses a continuous challenge for studies on pathogenesis, physiology, epidemiology and evolution of Campylobacter. In this chapter we will review the current state of the art of the genomics of thermophilic Campylobacter species, and opportunities where genomics can further contribute to our understanding of the biology of these successful human pathogens.

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A role for the tet(O) plasmid in maintaining Campylobacter plasticity

Cited by 21 publications

References 47 publications

A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

New molecular microbiology approaches in the study of Campylobacter fetus

Genomics of Thermophilic <i>Campylobacter</i> Species

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