A Role for the RNA Polymerase Gene Specificity Factor σ
            <sup>54</sup>
            in the Uniform Colony Growth of Uropathogenic Escherichia coli

Switzer, Amy; Burchell, Lynn; Mitsidis, Panagiotis; Thurston, Teresa L. M.; Wigneshweraraj, Sivaramesh

doi:10.1128/jb.00031-22

J Bacteriol

2022

DOI: 10.1128/jb.00031-22

|View full text |Cite

A Role for the RNA Polymerase Gene Specificity Factor σ ⁵⁴ in the Uniform Colony Growth of Uropathogenic Escherichia coli

Amy Switzer

Lynn Burchell

Panagiotis Mitsidis

et al.

Abstract: RNA synthesis is the first step of gene expression. The multisubunit RNA polymerase (RNAP) is the central enzyme responsible for RNA synthesis in bacteria.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Sousa,

Felice,

Dominici

et al. 2024

Venereology

View full text Add to dashboard Cite

Gardnerella vaginalis is a Gram-variable bacillus capable of causing bacterial vaginosis, a condition prevalent in reproductive-age women, this bacterium is present in almost 100% of cases and is also considered a gateway to various sexually transmitted infections. This organism exhibits high pathogenicity linked to virulence and resistance genes acquired throughout evolution, showcasing elevated resistance to a broad spectrum of drug classes. This study conducted comparative genomic analyses to identify these genes and correlate their presence with positive Darwinian selection. Additionally, new drug targets were selected through docking and molecular modeling, guided by the heightened antimicrobial resistance exhibited by this microbial species. The available genomes of G. vaginalis were analyzed, and the orthologous genes were delineated and positively selected, whereby 29 groups were found. Of these genes, one of great importance was predicted, Mef(A), which is related to resistance to the macrolide group of antibiotics, which are one of the main choices for the treatment of sexually transmitted infections. Additionally, two potential protein candidates were selected as drug targets. These proteins were linked with a natural compound each and are considered good potential drug targets. The analyses in this study contribute to analyzing the evolution of the species and how resistance genes are related to their permanence as a potential pathogen.

show abstract

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Sousa,

Felice,

Dominici

et al. 2024

Venereology

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Role for the RNA Polymerase Gene Specificity Factor σ ⁵⁴ in the Uniform Colony Growth of Uropathogenic Escherichia coli

Abstract: RNA synthesis is the first step of gene expression. The multisubunit RNA polymerase (RNAP) is the central enzyme responsible for RNA synthesis in bacteria.

Cited by 1 publication

References 33 publications

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Contact Info

Product

Resources

About

A Role for the RNA Polymerase Gene Specificity Factor σ 54 in the Uniform Colony Growth of Uropathogenic Escherichia coli

Abstract: RNA synthesis is the first step of gene expression. The multisubunit RNA polymerase (RNAP) is the central enzyme responsible for RNA synthesis in bacteria.

Cited by 1 publication

References 33 publications

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Unveiling Resistance and Virulence Mechanisms under Darwinian Positive Selection for Novel Drug Discovery for Gardnerella vaginalis

Contact Info

Product

Resources

About

A Role for the RNA Polymerase Gene Specificity Factor σ ⁵⁴ in the Uniform Colony Growth of Uropathogenic Escherichia coli