A role for the mycotoxin deoxynivalenol in stem colonisation during crown rot disease of wheat caused by Fusarium graminearum and Fusarium pseudograminearum

Mudge, Agnieszka M.; Dill‐Macky, Ruth; Dong, Yanhong; Gardiner, Donald M.; White, Rosemary G.; Manners, John M.

doi:10.1016/j.pmpp.2007.01.003

Cited by 161 publications

(207 citation statements)

References 40 publications

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“…The present study showed as in barley, differently from what reported by Mudge et al (2006) in soft wheat, the colonization by F. pseudograminearum strain CS3427 was limited to the basal portions (up to segment II), in accordance to what reported by Backhouse et al (2004) in FCR investigations conducted in Australia.…”

Section: Discussionsupporting

confidence: 90%

“…As already shown in wheat (Mudge et al 2006, Covarelli et al 2012, Winter et al 2013, Moretti et al 2014, FCR is a potential additional source of grain contamination with Fusarium mycotoxins. Thus, the objective of this study was to investigate the ability of F. graminearum, F. culmorum and F. pseudograminearum to systemically colonize barley plants starting from the stem base by visual, scanning electron microscope and Real-Time PCR methods and to verify the possible DON accumulation in the head.…”

Section: Manuscript Received December 2017mentioning

confidence: 65%

“…These data are related with the quantification of F. culmorum strain Fc820 biomass and also with the work of Covarelli et al (2012) that showed a decreasing concentration of DON from the stem-base to the head of soft wheat. DON translocation from the base to the head was also detected in soft wheat plants inoculated at the stem-base with F. graminearum and F. pseudograminearum (Mudge et al 2006) and it was observed how this characteristic is depending on strain aggressiveness, such as symptoms extension and fungal colonization (Beccari et al 2018). In addition, Mudge et al (2006) demonstrated how strains F. graminearum mutant for tri5 gene (responsible for DON biosynthesis) were unable to colonize the adjacent area of the flag leaf in soft wheat.…”

Section: Discussionmentioning

confidence: 96%

“…This finding was also confirmed by SEM observations and is partially in accordance with what described by other authors. The ability of Fusarium species, to systemically move along the stem up to the head, following stem-base infection, was already investigated on soft wheat (Mudge et al 2006, Covarelli et al 2012, Winter et al 2013) and durum wheat (Moretti et al 2014). …”

Section: Discussionmentioning

confidence: 99%

“…Conversely, for FCR, the relationship between symptom severity, fungal biomass and mycotoxin accumulation is still poorly understood, because Fusarium causal agents do not directly colonize the head. Recently, several studies (Mudge et al 2006, Covarelli et al 2012, Winter et al 2013, Moretti et al 2014, Beccari et al 2018) investigated the colonization process of different Fusarium species and their possible systemic movement along soft and durum wheat plants after stem base inoculation. In particular, Covarelli et al (2012), Winter et al (2013) and Moretti et al (2014), investigating also DON translocation during FCR progress, revealed the presence of this secondary metabolite in wheat heads and in the grains, even when Fusarium was not detected in these parts of the plant.…”

Section: Manuscript Received December 2017mentioning

confidence: 99%

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Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

Pecoraro

Giannini

Beccari

et al. 2018

AFSci

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Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents.

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Section: Discussionsupporting

confidence: 90%

Section: Manuscript Received December 2017mentioning

confidence: 65%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Manuscript Received December 2017mentioning

confidence: 99%

See 3 more Smart Citations

Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

Pecoraro

Giannini

Beccari

et al. 2018

AFSci

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Fusarium Diseases of Canadian Grain Crops: Impact and Disease Management Strategies

et al. 2014

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The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

Jubault

Canning

Urban

et al. 2011

Plant Fungal Pathogens

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In recent years, many Fusarium species have emerged which now threaten the productivity and safety of small grain cereal crops worldwide. During floral infection and post-harvest on stored grains the Fusarium hyphae produce various types of harmful mycotoxins which subsequently contaminate food and feed products. This article focuses specifically on the induction and production of the type B sesquiterpenoid trichothecene mycotoxins. Methods are described which permit in liquid culture the small or large scale production and detection of deoxynivalenol (DON) and its various acetylated derivatives. A wheat (Triticum aestivum L.) ear inoculation assay is also explained which allows the direct comparison of mycotoxin production by species, chemotypes and strains with different growth rates and/or disease-causing abilities. Each of these methods is robust and can be used for either detailed time-course studies or end-point analyses. Various analytical methods are available to quantify the levels of DON, 3A-DON and 15A-DON. Some criteria to be considered when making selections between the different analytical methods available are briefly discussed.

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A role for the mycotoxin deoxynivalenol in stem colonisation during crown rot disease of wheat caused by Fusarium graminearum and Fusarium pseudograminearum

Cited by 161 publications

References 40 publications

Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

Fusarium Diseases of Canadian Grain Crops: Impact and Disease Management Strategies

The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

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