A role for synaptopodin and the spine apparatus in hippocampal synaptic plasticity

Deller, Thomas; Bas‐Orth, Carlos; Turco, Domenico Del; Vlachos, Andreas; Burbach, Guido J.; Drakew, Alexander; Chabanis, Sophie; Körte, Martin; Schwegler, Herbert; Haas, Carola A.; Frotscher, Michael

doi:10.1016/j.aanat.2006.06.013

Cited by 60 publications

(46 citation statements)

References 75 publications

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“…On one hand, SP has been associated with the actin cytoskeleton and actin-regulatory proteins (Mundel et al, 1997;Asanuma et al, 2005), which suggest a role for SP in spine motility as well as GluR trafficking and anchoring (Wyszynski et al, 1997(Wyszynski et al, , 1998) but on the other hand, it has been hypothesized to be related to regulation of calcium stores (Deller et al, 2007;Jedlicka et al, 2008b), which are also associated with synaptic plasticity (Fitzjohn and Collingridge, 2002). Our results clearly link SP with calcium stores, immunolabeled with RyR.…”

Section: Discussionsupporting

confidence: 58%

Synaptopodin Regulates Plasticity of Dendritic Spines in Hippocampal Neurons

Vlachos

Korkotian²,

Schonfeld³

et al. 2009

J. Neurosci.

167

217

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The spine apparatus is an essential component of dendritic spines of cortical and hippocampal neurons, yet its functions are still enigmatic. Synaptopodin (SP), an actin-binding protein, is tightly associated with the spine apparatus and it may play a role in synaptic plasticity, but it has not yet been linked mechanistically to synaptic functions. We studied endogenous and transfected SP in dendritic spines of cultured hippocampal neurons and found that spines containing SP generate larger responses to flash photolysis of caged glutamate than SP-negative ones. An NMDA-receptor-mediated chemical long-term potentiation caused the accumulation of GFP-GluR1 in spine heads of control but not of shRNA-transfected, SP-deficient neurons. SP is linked to calcium stores, because their pharmacological blockade eliminated SP-related enhancement of glutamate responses, and release of calcium from stores produced an SP-dependent increase of GluR1 in spines. Thus, SP plays a crucial role in the calcium store-associated ability of neurons to undergo long-term plasticity.

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Section: Discussionsupporting

confidence: 58%

Synaptopodin Regulates Plasticity of Dendritic Spines in Hippocampal Neurons

Vlachos

Korkotian²,

Schonfeld³

et al. 2009

J. Neurosci.

167

217

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show abstract

“…Moreover, we were recently able to demonstrate that (3) SP/ SA is also involved in the regulation of homeostatic synaptic plasticity , which indicates that SP/SA could play an important role in adjusting the ability of neurons to express different forms of synaptic plasticity (Vlachos, 2012). Consistent with work on ER Ca 2+ stores in synaptic plasticity, these studies further established SP/SA as a key regulator of synaptic plasticity (for reviews see Deller et al, 2007;Jedlicka et al, 2008;Segal et al, 2010;Vlachos, 2012). While the precise cellular and molecular mechanisms through which SP/SA affect different forms of synaptic plasticity warrant additional investigation, experimental evidence has been provided for changes in SPexpression, SP-cluster sizes and stacking of SAs to accompany the induction of synaptic plasticity (Vlachos et al, 2009; see also Yamazaki et al, 2001;Fukazawa et al, 2003).…”

Section: The Sa Controls Different Forms Of Synaptic Plasticitymentioning

confidence: 67%

Synaptic plasticity at the interface of health and disease: New insights on the role of endoplasmic reticulum intracellular calcium stores

Maggio

Vlachos

2014

Neuroscience

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“…We examine here a cross-section of studies that describe this fascinating and poorly understood family of proteins, the synaptopodin family. Because some aspects of other members of the synaptopodin family have previously been reviewed (Deller et al 2007), we place emphasis here on synaptopodin 2.…”

Section: Introductionmentioning

confidence: 99%

Synaptopodin family of natively unfolded, actin binding proteins: physical properties and potential biological functions

Chalovich

Schroeter

2010

Biophys Rev

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The synaptopodin family of proteins consists of at least 3 members: synaptopodin, the synaptopodin 2 proteins, and the synaptopodin 2-like proteins. Each family member has at least 3 isoforms that are produced by alternative splicing. Synaptopodin family members are basic proteins that are rich in proline and have little regular 2° or 3° structure at physiological temperature, pH and ionic strength. Like other natively unfolded proteins, synaptopodin family members have multiple binding partners including actin and other actin-binding proteins. Several members of the synaptopodin family have been shown to stimulate actin polymerization and to bundle actin filaments either on their own or in collaboration with other proteins. Synaptopodin 2 has been shown to accelerate nucleation of actin filament formation and to induce actin bundling. The actin polymerization activity is inhibited by Ca-calmodulin. Synaptopodin 2 proteins are localized in Z-bands of striated and heart muscle and dense bodies of smooth muscle cells. Depending on the developmental status and stress, at least one member of the synaptopodin family can occupy nuclei of some cells. Members of the synaptopodin 2 subfamily have been implicated in cancers.

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A role for synaptopodin and the spine apparatus in hippocampal synaptic plasticity

Cited by 60 publications

References 75 publications

Synaptopodin Regulates Plasticity of Dendritic Spines in Hippocampal Neurons

Synaptopodin Regulates Plasticity of Dendritic Spines in Hippocampal Neurons

Synaptic plasticity at the interface of health and disease: New insights on the role of endoplasmic reticulum intracellular calcium stores

Synaptopodin family of natively unfolded, actin binding proteins: physical properties and potential biological functions

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