A role for lactate in the differentiation of thyroid cells in culture?

Mothersill, Carmel; Murphy, Ann; O’Connor, Michael K.

doi:10.1042/bst0090306

Cited by 2 publications

(9 citation statements)

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“…The pathway by which the lactate is being used is unknown. However, investigations using monofluoroacetic acid or 2,4 DNP, inhibitors of the citric acid cycle and of the respiratory chain respectively, at a wide range of concentrations (0.0001 -10 mM) suggest that, contrary to what was previously thought (Mothersill et al 1981), aerobic respiration is not involved in the disap¬ pearance of lactate from the medium since the substance had no effect on lactate use (Table 3) Glucose and lactate levels detected in medium samples during the life span of primary thyroid cultures maintained for 100 days without loss of differentiation. (Fig.…”

Section: Resultsmentioning

confidence: 50%

“…Preliminary investigation (Mothersill et al 1981) suggested that addition of concentrated a.D(+) glucose solution (Sigma: 5 mg in 0.1 ml per 5 ml medium) to the thyroid cultures at the point where medium lactate levels were almost exhausted re¬ sulted in an extension of the differentiated culture life span. In the present paper an attempt was made to extend the life span of differentiated thyroid cultures as far as possible.…”

Section: Resultsmentioning

confidence: 99%

“…The methods involved in the intitial establishment of the culture have been described elsewhere in some detail (O'Connor et al 1980;Mothersill et al 1981). Minor modifications to the original technique are incorporated in the abbreviated description which follows:…”

Section: Methodsmentioning

confidence: 99%

“…How¬ ever, none of these systems permits the long-term study of primary cultures because they involve either disruption of the cell layer and/or medium changes which alter the established extra cellular environment. Our group recently reported con¬ siderable success in extending both morphological and functional differentiation in primary sheep thyroid cultures for up to 30 days (O'Connor et al 1980;Mothersill et al 1981). The major condition necessary to achieve this is that the cultures of thyroid cells are kept without medium changes.…”

mentioning

confidence: 98%

“…In the absence of medium changes glucose levels fall rapidly and this fall is accompanied by a stoiciometric rise in the lactate level. When all the glucose has been exhausted the lactate level begins to decline and cell death is associated with the com¬ plete exhaustion of medium lactate (Mothersill et al 1981) at about day 30 of the primary culture.…”

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confidence: 99%

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Maintenance of differentiated sheep thyroid cells in primary culture for three months

Mothersill

Seymour

Malone³

1984

Acta Endocrinologica

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A method is described which permits culture of primary thyroid cells without subculture for at least 100 days. Cultures are maintained without medium changes for the entire period, and concentrated glucose is added to replenish energy supplies at carefully defined intervals. The cells retain morphological and functional differentiation shown by light and electron microscopy, PAS positive histochemistry, iodine uptake and T4 production for at least 100 days. After this time fairly sudden death of the cultures occurs. Possible mechanisms for the effect are postulated. The technique should make it possible to study long-term effects of drugs/radiation on differentiated cultures without the need for continuous subculture.The development of tissue culture systems which are capable of retaining the morphological and functional properties of differentiated mammalian tissues is of considerable importance for biological and medical research. Such systems can act as experimental models of the parent tissue to allow it to be studied in isolation from the inevitably complex and undefined interactions it will have with its environment in vivo. Furthermore, good tissue culture models permit human studies to be performed on surgical specimens and by mini¬ mising distress to animals follow the trend away from controversial animal experimentation. A major problem with primary cultures of differen¬ tiated tissues is that even successfully established cultures lose their morphological and functional characteristics relatively rapidly. This is frequently followed by senescence or transformation of the culture, or else its domination by fibroblasts (Paul 1972). Consequently, cell culture models of differentiated tissues are very limited in their range of applications due to the short duration of the re¬ quired properties.

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Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Maintenance of differentiated sheep thyroid cells in primary culture for three months

Mothersill

Seymour

Malone³

1984

Acta Endocrinologica

Self Cite

View full text Add to dashboard Cite

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Long-term culture of differentiated human thyroid tissue

Mothersill

Seymour

Moriarty

et al. 1985

Acta Endocrinologica

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Human thyroid cells obtained during surgery have been maintained in monolayer culture for at least 2 months and without loss of morphological or functional differentiation. Samples as small as 0.5 g could be cultured but best results were obtained with samples of 5\ p=n-\ 10 g. The technique used was developed in this laboratory for sheep tissue and was applicable without significant modification to human tissue. It depends on the complete absence of media changes at any time during the culture period. Energy substrates are replenished by the addition of concentrated glucose solutions to the existing media at carefully monitored intervals. Differences in both morphology and function could be observed between cultures derived from patients with different diseases, suggesting that the technique could have predictive value.

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A role for lactate in the differentiation of thyroid cells in culture?

Cited by 2 publications

References 0 publications

Maintenance of differentiated sheep thyroid cells in primary culture for three months

Maintenance of differentiated sheep thyroid cells in primary culture for three months

Long-term culture of differentiated human thyroid tissue

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