A role for lactate in the differentiation of cultured sheep thyroid cells

Mothersill, Carmel; Murphy, Ann; O’Connor, Michael K.; Seymour, Colin; Malone, J. F.

doi:10.1016/0309-1651(81)90202-2

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…The inhibiting effect of glucose on differentiation is not restricted to HT-29 cells: primary cultures of chick embryo neuroretinal cells accumulate lens specific 6crystalline when cultured in low glucose but fail to do so if supplementary glucose is added (De Pomerai and Gali, 1982); glucose inhibits the functional differentiation of primary cultures of sheep thyroid cells (Mothersill et al, 1981). Along with the results in HT-29 cells, these data suggest that glucose metabolism is involved in the regulation of cell differentiation.…”

Section: -mentioning

confidence: 59%

Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT‐29 selected for growth in sugar‐free medium and its inhibition by glucose

Zweibaum

Pinto

Chevalier

et al. 1985

Journal Cellular Physiology

313

193

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In order to study the effect of glucose on the differentiation of cultured human colon cancer cells, a subpopulation of HT-29 cells was selected for its capacity to grow in the total absence of sugar. These cells (Glc-cells) exhibit, after confluency, an enterocytic differentiation, in contrast to cells grown with glucose (Glc+ cells), which always remain undifferentiated. The differentiation is characterized by a polarization of the cell layer with apical brush borders and tight junctions, and by the presence of sucrase-isomaltase. The differentiation of Glc- cells is reversible: the addition of glucose to postconfluent cultures of Glc- cells results in an inhibiting effect on the expression of sucrase-isomaltase; switching growing cultures of Glc- cells to the Glc+ medium for several passages results in a progressive reversion to the undifferentiated state, which is completed after seven passages. The dedifferentiation process is associated with a parallel, passage-related, increase in the rates of glucose consumption and lactic acid production, and decreases of intracellular glycogen content, which return to the values of the undifferentiated original Glc+ cells. The values of these metabolic parameters are correlated, at each passage, with the degree of dedifferentiation of the cells. When these dedifferentiated cells, after having been cultured in Glc+ medium for 20 passages, are switched back to the Glc- medium, they readily grow without mortality, and reexpress the same enterocytic differentiation as the parent Glc- cells. These results show that the capacity of this subpopulation to grow and differentiate in the absence of sugar is a stable characteristic. They further suggest that glucose metabolism interferes with the program of differentiation of HT-29 cells.

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Section: -mentioning

confidence: 59%

Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT‐29 selected for growth in sugar‐free medium and its inhibition by glucose

Zweibaum

Pinto

Chevalier

et al. 1985

Journal Cellular Physiology

313

193

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“…The level of differentiation obtainable is high, follicular development, T, production and iodine trapping being detectable [ 70, 721. Differentiation is dependent on thyrotropic hormone and on availability of lactate which is used in the process of follicle formation [73]. Absence of these substances leads to dedifferentiation and growth of cells.…”

Section: Development Of New Transformation Systemsmentioning

confidence: 99%

Radiation transformation studies: are they relevant to radiation protection problems?

Seymour

Mothersill

1988

J. Radiol. Prot.

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Radiation induced cancer is of considerable interest in the field of radiation protection. Because of the difficulties of studying carcinogenesis in humans, several in vitro systems are utilised. These cell transformation systems are reviewed, with particular emphasis on their relevance to human radiological protection problems. Most available systems use rodent fibroblasts. These are discussed in detail, since considerable amounts of radiation transformation dose response data have been derived from them. Attention is drawn to certain artefacts which can cause problems with interpretation of such data. The relevance of these systems is questionable because of species differences, particularly concerning life span and because most human tumours are derived from epithelial cells. New epithelial culture systems and three-dimensional tissue culture methods becoming available are discussed in the light of their potential for addressing radiation protection problems.

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“…The follicular morphology im¬ proved for up to 20 days after which the cells rapidly died due to lack of nutrients (Mothersill et al 1980). Differentiated cell life could be extended up to 30 days by using conditioned medium from 10-15 day cultures for medium changes after day 20 and further extension of the differentiated life span to cultures could be produced by adding concentrated glucose solutions (5 mg in 0.1 ml medium per flask) to the medium just before the cell death was due to occur (Mothersill et al 1981). …”

mentioning

confidence: 99%

An investigation of the optimum culture conditions for a differentiated culture of sheep thyroid cells

Murphy¹,

Mothersill

O’Connor³

et al. 1983

Acta Endocrinologica

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Primary cultures of differentiated sheep thyroid were investigated to assess the effect of varying the media, sera, hormones and the initial cell number plated on the culture life span. Iodide uptake and follicular morphology were used as indices of differentiation.It was found that the effect of variations in the type of medium and the concentration and type of serum used was very small. The addition of all the hormones used \p=n-\ insulin, hydrocortisone and thyrotrophic hormone \p=n-\ had a synergistic effect on the iodide trapping ability of the culture system. A cell number of approximately 1 \m=x\106 cells per 25 cm2 flask was found to be optimum for the development of differentiation in the cultures.

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A role for lactate in the differentiation of cultured sheep thyroid cells

Cited by 10 publications

References 11 publications

Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT‐29 selected for growth in sugar‐free medium and its inhibition by glucose

Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT‐29 selected for growth in sugar‐free medium and its inhibition by glucose

Radiation transformation studies: are they relevant to radiation protection problems?

An investigation of the optimum culture conditions for a differentiated culture of sheep thyroid cells

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