A role for <i>Drosophila Wnt‐4</i> in heart development

Tauc, Helen M; Mann, Tabea; Werner, Kathrin; Pandur, Petra

doi:10.1002/dvg.22021

Cited by 10 publications

(8 citation statements)

References 48 publications

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“…A mutation in the Wnt4 gene ( Wnt4 EMS23 ) was identified in this large-scale screen and was classified in the category of abnormal cardiac morphology. The cardiac phenotype of Wnt4 mutants was also described in an independent study (Tauc et al, 2012), and was interpreted as an early cardiac specification defect. However, our detailed analysis of the Wnt4 mutant phenotype and its expression pattern suggest that Wnt4 is not required for cardiac specification, but required for ostia development.…”

Section: Introductionmentioning

confidence: 70%

“…A previous study of Wnt4 in the Drosophila heart did not identify downstream Wnt signaling pathway components (Tauc et al, 2012). We used multiple approaches to demonstrate that Wnt4 functions through the canonical Wnt signaling pathway.…”

Section: Discussionmentioning

confidence: 97%

“…In Drosophila heart development, Wnt signaling activated by Wg is known to promote early stage cardiac cell specification. A previous study of Wnt4 in Drosophila heart development (Tauc et al, 2012) showed cardiac cell specification defects in Wnt4 mutants, using antibody staining against Eve. However, we did not observe the same defects in early stage 11 to 13 embryos (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Wnt4 is required for ostia development in the Drosophila heart

Chen

Zhu

et al. 2016

Developmental Biology

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The Drosophila ostia are valve-like structures in the heart with functional similarity to vertebrate cardiac valves. The Wnt/ -catenin signaling pathway is critical for valve development in zebrafish and mouse, but the key ligand(s) for valve induction remains unclear. We observed high levels of Wnt4 gene expression in Drosophila ostia progenitor cells, immediately prior to morphological differentiation of these cells associated with ostia formation. This differentiation was blocked in Wnt4 mutants and in flies expressing canonical Wnt signaling pathway inhibitors but not inhibitors of the planar cell polarity pathway. High levels of Wnt4 dependent activation of a canonical Wnt signaling reporter was observed specifically in ostia progenitor cells. In vertebrate valve formation Wnt signaling is active in cells undergoing early endothelial-mesenchymal transition (EMT) and the Wnt9 homologue of Drosophila Wnt4 is expressed in valve progenitors. In demonstrating an essential role for Wnt4 in ostia development we have identified similarities between molecular and cellular events associated with early EMT during vertebrate valve development and the differentiation and partial delamination of ostia progenitor cells in the process of ostia formation.

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Section: Introductionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Wnt4 is required for ostia development in the Drosophila heart

Chen

Zhu

et al. 2016

Developmental Biology

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“…To test this more directly, we overexpressed dWnt4 in piRNA mutants to see if it would rescue the CB differentiation phenotype. We used c587-GAL4 to drive UASt dWnt4 in dSETDB1 mutants and stained them with Vasa and 1B1 [ 47 ]. Additionally, we depleted dSETDB1 and piwi only in the somatic cells, while simultaneously expressing dWnt4 there.…”

Section: Resultsmentioning

confidence: 99%

Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila

et al. 2016

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Germline stem cell (GSC) self-renewal and differentiation are required for the sustained production of gametes. GSC differentiation in Drosophila oogenesis requires expression of the histone methyltransferase dSETDB1 by the somatic niche, however its function in this process is unknown. Here, we show that dSETDB1 is required for the expression of a Wnt ligand, Drosophila Wingless type mouse mammary virus integration site number 4 (dWnt4) in the somatic niche. dWnt4 signaling acts on the somatic niche cells to facilitate their encapsulation of the GSC daughter, which serves as a differentiation cue. dSETDB1 is known to repress transposable elements (TEs) to maintain genome integrity. Unexpectedly, we found that independent upregulation of TEs also downregulated dWnt4, leading to GSC differentiation defects. This suggests that dWnt4 expression is sensitive to the presence of TEs. Together our results reveal a chromatin-transposon-Wnt signaling axis that regulates stem cell fate.

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“…A recent study described enriched expression of a Wg paralog, DWnt4 , in the ostia cells (Tauc et al 2012), and it is likely that this factor also contributes to Wg signaling in the ostia and potentially to ostia formation. Moreover, since canonical Wg signaling is not sufficient for ostia specification, factors in addition to Wg signaling are necessary for ostia formation, and our studies place these yet-to-be defined factors genetically downstream of abd-A .…”

Section: Discussionmentioning

confidence: 99%

The canonical Wingless signaling pathway is required but not sufficient for inflow tract formation in the Drosophila melanogaster heart

Trujillo

Nodal

Lovato

et al. 2016

Developmental Biology

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The inflow tracts of the embryonic Drosophila cardiac tube, termed ostia, arise in its posterior three segments from cardiac cells that co-express the homeotic transcription factor Abdominal-A (abdA), the orphan nuclear receptor Seven-up (Svp), and the signaling molecule Wingless (Wg). To define the roles of these factors in inflow tract development, we assessed their function in inflow tract formation. We demonstrate, using several criteria, that abdA, svp, and wg are each critical for normal inflow tract formation. We further show that Wg acts in an autocrine manner to impact ostia fate, and that it mediates this effect at least partially through the canonical Wg signaling pathway. By contrast, neither wg expression nor Wg signaling are sufficient for inflow tract formation when expressed in anterior Svp cells that do not normally form inflow tracts in the embryo. Instead, ectopic abd-A expression throughout the cardiac tube is required for the formation of ectopic inflow tracts, indicating that autocrine Wg signaling must be supplemented by additional Hox-dependent factors to effect inflow tract formation. Taken together, these studies define important cellular and molecular events that contribute to cardiac inflow tract development in Drosophila. Given the broad conservation of the cardiac regulatory network through evolution, our studies provide insight into mechanisms of cardiac development in higher animals.

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A role for Drosophila Wnt‐4 in heart development

Cited by 10 publications

References 48 publications

Wnt4 is required for ostia development in the Drosophila heart

Wnt4 is required for ostia development in the Drosophila heart

Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila

The canonical Wingless signaling pathway is required but not sufficient for inflow tract formation in the Drosophila melanogaster heart

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