A Role for ETR1 in Hydrogen Peroxide Signaling in Stomatal Guard Cells

Desikan, Radhika; Hancock, John T.; Bright, Jo; Harrison, Judith; Weir, Iain; Hooley, Richard; Neill, Steven J.

doi:10.1104/pp.104.056994

Cited by 186 publications

(128 citation statements)

References 22 publications

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“…In addition to salt stress, ethylene receptor may also play roles in hydrogen peroxide signaling (Desikan et al, 2005). A CTR1-like protein kinase gene was also induced by salt stress (Shiozaki et al, 2005).…”

Section: Discussionmentioning

confidence: 92%

Modulation of Ethylene Responses Affects Plant Salt-Stress Responses

et al. 2006

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Ethylene signaling plays important roles in multiple aspects of plant growth and development. Its functions in abiotic stress responses remain largely unknown. Here, we report that alteration of ethylene signaling affected plant salt-stress responses. A type II ethylene receptor homolog gene NTHK1 (Nicotiana tabacum histidine kinase 1) from tobacco (N. tabacum) conferred salt sensitivity in NTHK1-transgenic Arabidopsis (Arabidopsis thaliana) plants as judged from the phenotypic change, the relative electrolyte leakage, and the relative root growth under salt stress. Ethylene precursor 1-aminocyclopropane-1-carboxylic acid suppressed the salt-sensitive phenotype. Analysis of Arabidopsis ethylene receptor gain-of-function mutants further suggests that receptor function may lead to salt-sensitive responses. Mutation of EIN2, a central component in ethylene signaling, also results in salt sensitivity, suggesting that EIN2-mediated signaling is beneficial for plant salt tolerance. Overexpression of the NTHK1 gene or the receptor gain-of-function activated expression of salt-responsive genes AtERF4 and Cor6.6. In addition, the transgene NTHK1 mRNA was accumulated under salt stress, suggesting a posttranscriptional regulatory mechanism. These findings imply that ethylene signaling may be required for plant salt tolerance.

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Section: Discussionmentioning

confidence: 92%

Modulation of Ethylene Responses Affects Plant Salt-Stress Responses

et al. 2006

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“…ROS are key components of several regulatory processes in plants, including stomatal closure and gravitropism, as well as plant responses to abiotic stresses and pathogen challenge (Desikan et al, 2005). As a result, considerable research has focused not only on the mechanisms of ROS generation and removal in plants, but also their functions during development , as well as during biotic or abiotic stress (Apel et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1

Jang

Choi

et al. 2012

Molecules and Cells

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A highly oxidative stress-tolerant japonica rice line was isolated by T-DNA insertion mutation followed by screening in the presence of 50 mM H 2 O 2 . The T-DNA insertion was mapped to locus Os09g0547500, the gene product of which was annotated as lysine decarboxylase-like protein (GenBank accession No. AK062595). We termed this gene OsLDC-like 1, for Oryza sativa lysine decarboxylase-like 1. The insertion site was in the second exon and resulted in a 27 amino acid N-terminal deletion. Despite this defect in OsLDC-like 1, the mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress. The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining. Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenouslyapplied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves. Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H 2 O 2 . Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype. These results suggest that increased cellular PA levels have a physiological role in preventing stress-induced ROS and ethylene accumulation and the resultant cell damage.

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“…Additional studies have suggested roles of ethylene in either stomatal opening or closing, depending on the plant species (Giulivo, 1986). Recently, it was reported that H 2 O 2 -mediated stomatal closure is completely disrupted in the loss-of-function mutant of the ethylene receptor etr1-7, suggesting a role for ETR1 in guard cell ROS signaling and stomatal closure (Desikan et al, 2005). Interestingly, in another recent study, ethylene was proposed to counteract stomatal closure (Tanaka et al, 2005).…”

mentioning

confidence: 99%

“…Moreover, this ethylene-induced inhibition of stomatal closure was suppressed in two ethylene-insensitive mutants, the dominant etr1-1 allele, and ein3-1 (Tanaka et al, 2005). Reverse transcription-PCR analysis with guard cellenriched epidermal strips showed that ETR1 is expressed in guard cells (Desikan et al, 2005). ATH1 and AG oligonucleotide-based (Affymetrix) microarray analyses of guard cell-expressed genes suggest that at least the ETR1, ERS1, and EIN4 ethylene receptor genes are expressed in guard cells, with ERS1 apparently showing the highest expression level in these experiments (Leonhardt et al, 2004;Heggie and Gray, 2005; J. Kwak, N. Leonhardt, Y. Yang, and J. Schroeder, unpublished data).…”

mentioning

confidence: 99%

“…As these receptors share overlapping redundant negative regulator functions (Hua and Meyerowitz, 1998), it would be interesting to analyze stomatal responses in etr1ein4, etr1ers1, and ers1ein4 double mutants in addition to the recessive loss-offunction etr1-7 line (Desikan et al, 2005). The dual functions of ethylene receptors proposed in these two studies-transduction of stomatal closure and inhibition of ABA-induced stomatal closure in Arabidopsis (Arabidopsis thaliana)-will require further analyses (Desikan et al, 2005;Tanaka et al, 2005). a well-developed system for studying cell biological and physiological functions of GA 3 responses.…”

mentioning

confidence: 99%

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The Role of Reactive Oxygen Species in Hormonal Responses

Kwak

Nguyen²,

Schroeder³

2006

Plant Physiology

304

193

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Reactive oxygen species (ROS) are versatile molecules mediating a variety of cellular responses in plant cells, including programmed cell death (PCD), development, gravitropism, and hormone signaling. A picture showing how ROS function in signal transduction networks has started to emerge as the result of recent studies providing genetic, cell biological, and physiological evidence describing roles for ROS in signaling (Apel and Hirt, 2004;Laloi et al., 2004;Mittler et al., 2004;Mori and Schroeder, 2004). However, further efforts are necessary to characterize the targets and molecular functions of ROS, as well as the complex interplay of ROS-generating and ROSscavenging mechanisms. Moreover, the interactions of nitric oxide with other ROS species in hormone signaling is a subject of interest (Desikan et al., 2004;Wendehenne et al., 2004;Guo and Crawford, 2005;Bright et al., 2006). Due to limited space, in this Update article we focus on recent progress made in understanding the roles of ROS in hormone signaling. AUXIN, ETHYLENE, AND ROSROS have been implicated as second messengers in several plant hormone responses. Joo et al. (2001) showed that ROS are asymmetrically generated in roots by gravistimulation to regions of reduced growth. A function for ROS in root curvature was reported by inhibiting cell growth, thus contributing to tropisms. Auxin also induced ROS production in roots and the auxin transport inhibitor N-1-naphthylphthlamic acid did not inhibit hydrogen peroxide (H 2 O 2 )-induced root curvature, leading to the suggestion that ROS play a role downstream of transport in auxin signaling and gravitropism (Joo et al., 2001).A pharmacological study suggested that ethylene and ROS are required for root nodule initiation and function as positive transducers downstream of the Nod factor response in a semiaquatic legume (D'Haeze et al., 2003). Additional studies have suggested roles of ethylene in either stomatal opening or closing, depending on the plant species (Giulivo, 1986). Recently, it was reported that H 2 O 2 -mediated stomatal closure is completely disrupted in the loss-of-function mutant of the ethylene receptor etr1-7, suggesting a role for ETR1 in guard cell ROS signaling and stomatal closure (Desikan et al., 2005). Interestingly, in another recent study, ethylene was proposed to counteract stomatal closure (Tanaka et al., 2005). Abscisic acid (ABA)-induced stomatal closure was inhibited by ethylene or the hormone precursor 1-aminocyclopropane-1-carboxylic acid and by the ethylene-overproducing mutation eto1-1 (Tanaka et al., 2005). Moreover, this ethylene-induced inhibition of stomatal closure was suppressed in two ethylene-insensitive mutants, the dominant etr1-1 allele, and ein3-1 (Tanaka et al., 2005). Reverse transcription-PCR analysis with guard cellenriched epidermal strips showed that ETR1 is expressed in guard cells (Desikan et al., 2005). ATH1 and AG oligonucleotide-based (Affymetrix) microarray analyses of guard cell-expressed genes suggest that at least the ETR1, ERS1, and EIN4 eth...

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A Role for ETR1 in Hydrogen Peroxide Signaling in Stomatal Guard Cells

Cited by 186 publications

References 22 publications

Modulation of Ethylene Responses Affects Plant Salt-Stress Responses

Modulation of Ethylene Responses Affects Plant Salt-Stress Responses

Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1

The Role of Reactive Oxygen Species in Hormonal Responses

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