A role for differential Rubisco activase isoform expression in C<sub>4</sub> bioenergy grasses at high temperature

Kim, Sang Yeol; Slattery, Rebecca; Ort, Donald R.

doi:10.1111/gcbb.12768

Cited by 24 publications

(24 citation statements)

References 75 publications

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“…Under WW38, no significant changes on the Rubisco activation state were observed ( Figures 4C,D ), which can be explained by the fact that in maize Rubisco is exclusively located in the chloroplast of BSC, surrounding the vascular tissue, that can offer a superior exposure to the evaporative cooling capacity, buffering the BSC temperature rise ( Lundgren et al, 2014 ; Pignon et al, 2019 ). Another possible explanation is that the long-term acclimation to high temperature experienced by these plants allowed the expression of Rca isoforms more active under high temperature repairing catalytic misfire inhibition, as reported in other studies ( Crafts-Brandner and Salvucci, 2002 ; Yin et al, 2014 ; Zhang et al, 2019 ; Kim et al, 2021 ).…”

Section: Discussionmentioning

confidence: 66%

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

et al. 2021

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Increasing temperatures and extended drought episodes are among the major constraints affecting food production. Maize has a relatively high temperature optimum for photosynthesis compared to C3 crops, however, the response of this important C4 crop to the combination of heat and drought stress is poorly understood. Here, we hypothesized that resilience to high temperature combined with water deficit (WD) would require efficient regulation of the photosynthetic traits of maize, including the C4–CO2 concentrating mechanism (CCM). Two genotypes of maize with contrasting levels of drought and heat tolerance, B73 and P0023, were acclimatized at high temperature (38°C versus 25°C) under well-watered (WW) or WD conditions. The photosynthetic performance was evaluated by gas exchange and chlorophyll a fluorescence, and in vitro activities of key enzymes for carboxylation (phosphoenolpyruvate carboxylase), decarboxylation (NADP-malic enzyme), and carbon fixation (Rubisco). Both genotypes successfully acclimatized to the high temperature, although with different mechanisms: while B73 maintained the photosynthetic rates by increasing stomatal conductance (gs), P0023 maintained gs and showed limited transpiration. When WD was experienced in combination with high temperatures, limited transpiration allowed water-savings and acted as a drought stress avoidance mechanism. The photosynthetic efficiency in P0023 was sustained by higher phosphorylated PEPC and electron transport rate (ETR) near vascular tissues, supplying chemical energy for an effective CCM. These results suggest that the key traits for drought and heat tolerance in maize are limited transpiration rate, allied with a synchronized regulation of the carbon assimilation metabolism. These findings can be exploited in future breeding efforts aimed at improving maize resilience to climate change.

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Section: Discussionmentioning

confidence: 66%

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

et al. 2021

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“…Plants grown in warm environments usually have RCAs that are more thermotolerant 89 – 91 . In S. viridis , maize, and sorghum, high temperature induces the protein level of RCA_α and the rate of RCA_α induction is associated with the recovery rate of Rubisco activation and photosynthesis 92 . Our results, taken with previously published reports, suggest the heat-induced RCA_α may be the thermotolerant isoform.…”

Section: Discussionmentioning

confidence: 99%

High light and temperature reduce photosynthetic efficiency through different mechanisms in the C4 model Setaria viridis

et al. 2021

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C4 plants frequently experience high light and high temperature conditions in the field, which reduce growth and yield. However, the mechanisms underlying these stress responses in C4 plants have been under-explored, especially the coordination between mesophyll (M) and bundle sheath (BS) cells. We investigated how the C4 model plant Setaria viridis responded to a four-hour high light or high temperature treatment at photosynthetic, transcriptomic, and ultrastructural levels. Although we observed a comparable reduction of photosynthetic efficiency in high light or high temperature treated leaves, detailed analysis of multi-level responses revealed important differences in key pathways and M/BS specificity responding to high light and high temperature. We provide a systematic analysis of high light and high temperature responses in S. viridis, reveal different acclimation strategies to these two stresses in C4 plants, discover unique light/temperature responses in C4 plants in comparison to C3 plants, and identify potential targets to improve abiotic stress tolerance in C4 crops.

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“…Exploiting temperature-induced differential expression of Rca is a potential strategy to accomplish this objective. In many crops, Rca consists of multiple protein isoforms with differing heat sensitivity ( Crafts-Brandner et al , 1997 ; Law et al , 2001 ; Law and Crafts-Brandner, 2001 ; Carmo-Silva et al , 2015 ; Scafaro et al , 2019 ; Kim et al , 2020 ). In bread wheat, altered thermal tolerance between Rca isoforms is conferred by a single amino acid substitution that acts as a thermal and regulatory switch, providing a compelling target for future genome editing efforts ( Scafaro et al , 2019 ; Degen et al , 2020 ).…”

Section: Temperature Response Of Photosynthesis Within the Leaf: The mentioning

confidence: 99%

The effect of increasing temperature on crop photosynthesis: from enzymes to ecosystems

Moore

Meacham-Hensold

Lemonnier

et al. 2021

Journal of Experimental Botany

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As global land surface temperature continues to rise and heat wave events increase in frequency, duration and/or intensity, our key food and fuel cropping systems will likely face increased heat-related stress. A large volume of literature exists on exploring measured and modelled impacts of rising temperature on crop photosynthesis, from enzymatic responses within the leaf up to larger ecosystem scale responses that reflect seasonal and inter-annual crop responses to heat. This review discusses (i) how crop photosynthesis changes with temperature at the enzymatic scale within the leaf, (ii) how stomata and plant transport systems are affected by temperature, (iii) what features make a plant susceptible or tolerant to elevated temperature and heat stress, and (iv) how these temperature and heat effects compound at the ecosystem scale to affect crop yields. Throughout the review, we identify current advancements and future research trajectories that are needed to make our cropping systems more resilient to rising temperature and heat stress, which are both projected to occur due to current global fossil fuel emissions.

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A role for differential Rubisco activase isoform expression in C₄ bioenergy grasses at high temperature

Cited by 24 publications

References 75 publications

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

High light and temperature reduce photosynthetic efficiency through different mechanisms in the C4 model Setaria viridis

The effect of increasing temperature on crop photosynthesis: from enzymes to ecosystems

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A role for differential Rubisco activase isoform expression in C4 bioenergy grasses at high temperature

Cited by 24 publications

References 75 publications

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize

High light and temperature reduce photosynthetic efficiency through different mechanisms in the C4 model Setaria viridis

The effect of increasing temperature on crop photosynthesis: from enzymes to ecosystems

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A role for differential Rubisco activase isoform expression in C₄ bioenergy grasses at high temperature