A role for active oxygen species as second messengers in the induction of alternative oxidase gene expression in <i>Petunia hybrida</i> cells

Wagner, Anneke M.

doi:10.1016/0014-5793(95)00688-6

Cited by 191 publications

(111 citation statements)

References 24 publications

(33 reference statements)

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“…However, it is not known what role this enzyme serves in other plants, including maize. One compelling idea is that AOX can control the production of free radicals by stabilizing the ratio of reduced ubiquinone to total ubiquinone (Purvis and Shewfelt, 1993;Wagner, 1995;Wagner and Moore, 1997;Millenaar et al, 1998). Indeed, isolated mitochondria were found to produce more H 2 O 2 when the alternative pathway was inhibited (Popov et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Alternative Oxidase Genes in Maize Mitochondrial Mutants

et al. 2002

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We have examined the expression of three alternative oxidase ( aox ) genes in two types of maize mitochondrial mutants. Nonchromosomal stripe (NCS) mutants carry mitochondrial DNA deletions that affect subunits of respiratory complexes and show constitutively defective growth. Cytoplasmic male-sterile (CMS) mutants have mitochondrial DNA rearrangements, but they are impaired for mitochondrial function only during anther development. In contrast to normal plants, which have very low levels of AOX, NCS mutants exhibit high expression of aox genes in all nonphotosynthetic tissues tested. The expression pattern is specific for each type of mitochondrial lesion: the NADH dehydrogenase-defective NCS2 mutant has high expression of aox2 , whereas the cytochrome oxidase-defective NCS6 mutant predominantly expresses aox3 . Similarly, aox2 and aox3 can be induced differentially in normal maize seedlings by specific inhibitors of these two respiratory complexes. Translation-defective NCS4 plants show induction of both aox2 and aox3 . AOX2 and AOX3 proteins differ in their ability to be regulated by reversible dimerization. CMS mutants show relatively high levels of aox2 mRNAs in young tassels but none in ear shoots. Significant expression of aox1 is detected only in NCS and CMS tassels. The induction pattern of maize aox genes could serve as a selective marker for diverse mitochondrial defects.

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Section: Discussionmentioning

confidence: 99%

Differential Expression of Alternative Oxidase Genes in Maize Mitochondrial Mutants

et al. 2002

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“…That the Aox1/Ars reporter was not activated when cells were treated with antimycin A or H 2 O 2 was surprising to us given the wealth of previous research from higher plants indicating that Aox1 is strongly induced by both antimycin A (Vanlerberghe and McIntosh 1994) and H 2 O 2 (Wagner 1995). Because of this disparity, we undertook a series of experiments examining Aox1 expression using the more traditional approach of measuring changes in mRNA abundance.…”

Section: Kinetics Of Aox1 Transcript Accumulationmentioning

confidence: 98%

“…In both soybean (Finnegan et al 1997) and Arabidopsis (Saisho et al 1997), each Aox gene shows differential developmental and tissue-specific expression. AOX abundance is transcriptionally controlled with a wide range of stress factors being shown to induce Aox gene expression and/or AOX protein accumulation including chilling (Vanlerberghe and McIntosh 1992), ageing (Hiser and McIntosh 1990), pathogen attack (Simons et al 1999), as well as treating tissues with salicylic acid (Rhoads and McIntosh 1992), H 2 O 2 (Wagner 1995) and the CP inhibitor antimycin A (Vanlerberghe and McIntosh 1994). A common consequence of many of these stresses is that they elevate intracellular ROS levels, which in turn, may represent a common signalling molecule upregulating Aox gene expression (Maxwell et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the alternative oxidase of Chlamydomonas reinhardtii in response to oxidative stress and a shift in nitrogen source

Molen

Rosso

Piercy

et al. 2006

Physiologia Plantarum

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To study the transcriptional regulation of Aox1, the major alternative oxidase (AOX) gene, we fused 1072 bp of its promoter to the promoterless arylsulfatase reporter gene. We find that the reporter is strongly activated when cells are shifted from a medium containing ammonium to one containing nitrate but is unresponsive to treatments known to induce Aox1 in higher plants -H 2 O 2 , antimycin A and cold stress. However, induction of Aox1 by all these factors was found when changes in gene expression were monitored using RNA blot analysis. Our data suggest that transcriptional upregulation of Aox1 by H 2 O 2 , antimycin A and cold-stress requires one or more enhancers which are not found in the proximal promoter region of the gene. Interestingly, while nitrate, H 2 O 2 and cold stress result in increased AOX abundance and increased alternative pathway respiration, these changes are not seen when cells are treated with antimycin A. We find that H 2 O 2 , antimycin A and cold-stress result in an increase in intracellular reactive oxygen species (ROS) but that a significant change in ROS does not occur when cells are shifted into a medium-containing nitrate. Overall, our data are consistent with there being two distinct pathways regulating Aox1 transcription and AOX abundance in Chlamydomonas reinhardtii: one in response to oxidative stress and a second due to metabolic changes brought about by a shift in nitrogen source from ammonium to nitrate.

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“…On one hand, high concentration of ROS can lead to phytotoxicity; on the other hand, ROS, particularly hydrogen peroxide (H 2 O 2 ), can be used for acclamatory signaling to activate antioxidant defense systems (Dat et al 2000). Wagner (1995) found that addition of exogenous H 2 O 2 into Petunia hybrida cells resulted Induction of AOX1a gene under salt stress is mediated by H2O2 869 in an increase in both the cyanide-resistant respiration and AOX protein amount, suggesting that H 2 O 2 could be used as secondary messenger in the signal transduction pathway to induce the AOX pathway. However, whether the accumulation of H 2 O 2 under the condition of salt stress would regulate the AOX pathway is still to be determined.…”

Section: Introductionmentioning

confidence: 99%

Salt stress-induced expression of rice AOX1a is mediated through an accumulation of hydrogen peroxide

Feng

Wang

et al. 2010

Biologia

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Treatment with 300 mM NaCl increased the capacity of the alternative respiratory pathway and induced the expression of AOX1a of the leaves of rice (Oryza sativa L.) seedlings. A significant increase in the content of hydrogen peroxide (H2O2) was observed in rice leaves treated with 300 mM NaCl. However, NaCl at 150 mM did not significantly affect the capacity of the alternative respiratory pathway, the content of H2O2, and the transcript level of AOX1a. Exogenous application of H2O2 enhanced the levels of the capacity of the alternative respiratory pathway and AOX1a expression. The accumulation of H2O2 in rice leaves in response to 300 mM NaCl was inhibited by the pretreatment with dimethylthiourea (DMTU, scavenger of H2O2). This treatment also suppressed the induction of AOX1a expression and the increase in the capacity of the alternative respiratory pathway under 300 mM NaCl stress. Moreover, the salt-stressed (300 mM NaCl) seedlings pretreated with 1 mM salicylhydroxamic acid (SHAM, a special inhibitor of alternative oxidase) had higher level of H2O2 production than the seedlings either subjected to 300 mM NaCl stress or SHAM treatment alone did. These observations suggest that the expression of AOX1a in response to higher salt stress is mediated through an accumulation of H2O2 and alternative oxidase could play a role in antioxidant protection under the condition of higher salt stress.

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A role for active oxygen species as second messengers in the induction of alternative oxidase gene expression in Petunia hybrida cells

Cited by 191 publications

References 24 publications

Differential Expression of Alternative Oxidase Genes in Maize Mitochondrial Mutants

Differential Expression of Alternative Oxidase Genes in Maize Mitochondrial Mutants

Characterization of the alternative oxidase of Chlamydomonas reinhardtii in response to oxidative stress and a shift in nitrogen source

Salt stress-induced expression of rice AOX1a is mediated through an accumulation of hydrogen peroxide

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