A role for a conserved kinase in the transcriptional control of methionine biosynthesis in<i>Escherichia coli</i>experiencing sustained nitrogen starvation

Switzer, Amy; Evangelopoulos, Dimitrios; Figueira, R.C.S.; Carvalho, Luiz Pedro S. de; Brown, Daniel R.; Wigneshweraraj, Sivaramesh

doi:10.1101/254623

2018

DOI: 10.1101/254623

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A role for a conserved kinase in the transcriptional control of methionine biosynthesis inEscherichia coliexperiencing sustained nitrogen starvation

Amy Switzer

Dimitrios Evangelopoulos

R.C.S. Figueira

et al.

Abstract: The initial adaptive transcriptional response to nitrogen (N) starvation in Escherichia coli involves large-scale alterations to the transcriptome mediated by the transcription activator, NtrC. One of the NtrC-activated genes is yeaG, which encodes a conserved

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Global RNA interactome of nitrogen starvedEscherichia coliuncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery

McQuail,

Matera,

Gräfenhan

et al. 2023

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The RNA binding protein Hfq has a central role in the post-transcription control of gene expression in many bacteria. Numerous studies have mapped the transcriptome-wide Hfq-mediated RNA-RNA interactions in growing bacteria or bacteria that have entered short-term growth-arrest. To what extent post-transcriptional regulation underpins gene expression in growth-arrested bacteria remains unknown. Here, we used nitrogen (N) starvation as a model to study the Hfq-mediated RNA interactome asEscherichia colienter, experience, and exit long-term growth arrest. We observe that the Hfq-mediated RNA interactome undergoes extensive changes during N starvation, with the conserved SdsR sRNA making the most interactions with different mRNA targets exclusively in long-term N-starvedE. coli. Taking a proteomics approach, we reveal that in growth-arrested cells SdsR influences gene expression far beyond its direct mRNA targets. We demonstrate that the absence of SdsR significantly compromises the ability of the mutant bacteria to recover growth competitively from the long-term N-starved state and uncover a conserved post-transcriptional regulatory axis which underpins this process.

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Global RNA interactome of nitrogen starvedEscherichia coliuncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery

McQuail,

Matera,

Gräfenhan

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

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A role for a conserved kinase in the transcriptional control of methionine biosynthesis inEscherichia coliexperiencing sustained nitrogen starvation

Abstract: The initial adaptive transcriptional response to nitrogen (N) starvation in Escherichia coli involves large-scale alterations to the transcriptome mediated by the transcription activator, NtrC. One of the NtrC-activated genes is yeaG, which encodes a conserved

Cited by 1 publication

References 19 publications

Global RNA interactome of nitrogen starvedEscherichia coliuncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery

Global RNA interactome of nitrogen starvedEscherichia coliuncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery

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