A robust set of black walnut microsatellites for parentage and clonal identification

Genetic consequences of harvest in a mature second-growth stand of black walnut (Juglans nigra L.)

“…For example, Victory et al (2006) observed comparable levels of genetic diversity (A r = 22.91; H E = 0.793) averaged across loci in a range-wide survey of 43 populations of black walnut using the same 12 microsatellites used in this study. Robichaud et al (2006) estimated similar levels of genetic diversity to those reported here (A r = 19.90, H E = 0.832) in a 40 year-old black walnut provenance/progeny trial of 39 open-pollinated families representing a broad sampling from throughout the native range of black walnut. Estimates of A r and H E based on microsatellite data for other temperate hardwood tree species were similar to those observed in the Long Hollow black walnut population (Dow and Ashley, 1996;van der Schoot et al, 2000).…”

“…Results from studies of other black walnut populations have found high levels of genetic diversity in black walnut seeds using allozymes (H E = 0.22−0.34) (Busov et al, 2002;Rink et al, 1989;Rink et al, 1994) and adult trees using microsatellites (Robichaud et al, 2006;Victory et al, 2006). For example, Victory et al (2006) observed comparable levels of genetic diversity (A r = 22.91; H E = 0.793) averaged across loci in a range-wide survey of 43 populations of black walnut using the same 12 microsatellites used in this study.…”

Walnut: past and future of genetic improvement

“…The following sections depict an overview of these molecular studies and their application (Table 2). Woeste et al 2002;Dangl et al 2005;Hoban et al 2008;Zhang et al 2010;Qi et al 2011;Yi et al 2011;Wu et al 2012;Zhang et al 2013;Chen et al 2013;Najafi et al 2014;Topçu et al 2015;Ikhsan et al 2016;Dang et al 2015;Hu et al 2015;Dang et al 2016 Interspecific hybrids identification Pollegioni et al 2008Pollegioni et al , 2009 Genetic diversity/relationships determination Foroni et al 2005;Dangl et al 2005;Victory et al 2006;Robichaud et al 2006;Wang et al 2008;Aradhya et al 2009;Gunn et al 2010;Karimi et al 2010;Bai et al 2010;Mohsenipoor et al 2010;Ebrahimi et al 2011;Ruiz-Garcia et al 2011;Pollegioni et al 2011;Qi et al 2011;Grauke et al 2012;Mahmoodi et al 2013;Zhang et al 2013;Pop et al 2013;Najafi et al 2014;Dang et al 2015;Hu et al 2015;Han et al 2016;Noor Shah et al 2016;Ćelepirović et al 2016;Wang et al 2016;…”

“…The first walnut SSR study was performed on J. nigra accessions to fingerprint clones with high-quality timber and edible nuts . Two additional studies using SSRs on J. nigra showed a low genetic diversity among several populations from the central hardwood region of the USA (Victory et al 2006) and identified parentage among progenies of several families (Robichaud et al 2006). Another study characterized J. nigra, J. regia, and natural hybrids between the two species Juglans × intermedia (Carr.)…”

SSR and RAPD analysis of genetic diversity in walnut (Juglans regia L.) genotypes from Jammu and Kashmir, India

“…Previously, in addition to morphological identification (Zenelli et al 2005), various biochemical and molecular markers have been used for genetic characterization of walnut genotypes. These included isozymes (Fornari et al 2001;Busov et al 2002;Ninot and Aleta 2003;Vyas et al 2003,), restriction fragment length polymorphisms (RFLPs) (Fjellstrom et al1995), randomly amplified polymorphic DNAs (RAPDs) (Nicese et al1998;Yan-Min et al 2000;Zhang Li et al 2007), intersimple sequence repeats (ISSRs) (Potter et al 2002), simple sequence repeats (SSRs) Dangl et al 2005;Foroni et al 2005;Victory et al 2006;Robichaud et al 2006;Karimi et al 2010), amplified fragment length polymorphisms (Kafkas et al 2005;Bayazit et al 2007) and SNPs (Ciarmiello et al 2011). In our study, the average number of alleles per locus was 2 which is higher than 1.3 detected in J. regia with RAPDs (Nicese et al 1998) but lesser than 3.9 and 5.5 using ISSR (Potter et al 2002) and SSR (Foroni et al 2007) markers respectively.…”