A robust polyacrylic acid/chitosan cryogel for rapid hemostasis

Shi, Mingyue; Jiang, Lijie; Yu, Chaojie; Dong, Xiaoru; Yu, Qingyu; Yao, Mengmeng; He, Shaoshuai; Yue, Zhiwei; Yao, Fanglian; Zhang, Hong; Sun, Hong; Li, Junjie

doi:10.1007/s11431-021-1986-9

Cited by 21 publications

(14 citation statements)

References 47 publications

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“…Cell Viability Assay: The cytocompatibility of the hydrogels was detected by CCK-8 assay (CA1210, Solarbio) according to standard protocols. [49,50] Briefly, hydrogel (1 g) was immersed in complete medium (10 mL) at 37 °C for 48 h to obtain the hydrogel extract. ADSCs were seeded in a 96-well tissue culture plate at a density of 1×10 4 well and incubated for 12 h. Then, the medium was replaced with hydrogel extract (200 µL) and cultured for another 24 and 48 h. Fresh medium was used as a control.…”

Section: Methodsmentioning

confidence: 99%

Ultra‐Histocompatible and Electrophysiological‐Adapted PEDOT‐Based Hydrogels Designed for Cardiac Repair

Yue

Zhang

et al. 2023

Adv Funct Materials

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Currently, although conducting polymers have exhibited potential electrophysiological modulation, designing bioinspired ultra‐histocompatible conducting polymers remains a long‐standing challenge. Moreover, the water dispersibility, conductivity, and biocompatibility of conducting polymers are incompatible, which restricts their application in tissue engineering. Herein, a multilevel template dispersion strategy is presented to produce poly(3,4‐ethylenedioxythiophene):(dextran sulfate/carboxymethyl chitosan) (PEDOT:(DSS/CMCS)) with biocompatibility superior to that of commercial poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate) without sacrificing processability and conductivity. The PEDOT:(DSS/CMCS) and oxidized dextran solutions form an injectable PEDOT‐based hydrogel (PDCOH) mediated by dynamic covalent imine bonds under mild conditions. The PDCOH has a tissue‐matched modulus and conductivity to adapt to the mechanical environment of dynamic tissue and modulate fibrosis‐induced electrical decoupling. The PDCOH combined with adipose‐derived stem cells demonstrates superior cardiac repair effects over cell suspensions and nonconductive hydrogels, inhibiting ventricular remodeling, reducing fibrous scarring, promoting vascular regeneration, and restoring electrophysiological and pulsatile functions.

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Section: Methodsmentioning

confidence: 99%

Ultra‐Histocompatible and Electrophysiological‐Adapted PEDOT‐Based Hydrogels Designed for Cardiac Repair

Yue

Zhang

et al. 2023

Adv Funct Materials

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“…The cytocompatibility of the hydrogels was detected by AO staining and CCK-8 assay (CA1210, Solarbio) using L929 mouse fibroblast cells according to previous standard protocols. , The raw material was sterilized by UV irradiation, and 1 g of hydrogel was immersed in a 10 mL of complete medium at 37 °C for 48 h to obtain the hydrogel extract. L929 cells were seeded in a 96-well tissue culture plate at a density of 1 × 10 4 /well and incubated for 12 h. Then, the medium was replaced with 200 μL of hydrogel extract and cultured for an additional 24 and 48 h. Cells cultured in fresh medium were used as a control.…”

Section: Methodsmentioning

confidence: 99%

“…Paraffin-embedded heart specimens were sliced into 4 μm-thick sections and placed onto slides. Masson (DC0032, Leagene Biotechnology) and Sirius red (DC0041, Leagene Biotechnology) staining were performed to assess myocardial morphology and fibrosis according to standard protocols . A fluorescence microscope (BX63, Olympus, Japan) captured images.…”

Section: Methodsmentioning

confidence: 99%

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An Intrapericardial Injectable Hydrogel Patch for Mechanical–Electrical Coupling with Infarcted Myocardium

Yue

Shi

et al. 2022

ACS Nano

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Although hydrogel-based patches have shown promising therapeutic efficacy in myocardial infarction (MI), synergistic mechanical, electrical, and biological cues are required to restore cardiac electrical conduction and diastolic–systolic function. Here, an injectable mechanical–electrical coupling hydrogel patch (MEHP) is developed via dynamic covalent/noncovalent cross-linking, appropriate for cell encapsulation and minimally invasive implantation into the pericardial cavity. Pericardial fixation and hydrogel self-adhesiveness properties enable the MEHP to highly compliant interfacial coupling with cyclically deformed myocardium. The self-adaptive MEHP inhibits ventricular dilation while assisting cardiac pulsatile function. The MEHP with the electrical conductivity and sensitivity to match myocardial tissue improves electrical connectivity between healthy and infarcted areas and increases electrical conduction velocity and synchronization. Overall, the MEHP combined with cell therapy effectively prevents ventricular fibrosis and remodeling, promotes neovascularization, and restores electrical propagation and synchronized pulsation, facilitating the clinical translation of cardiac tissue engineering.

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“…Stimulus-responsive polymers, which have the capabilities of responding to the environmental stimulations [1][2][3][4][5][6][7], have attracted great attention in the scientific community. Due to their numerous advantages, including designable properties [8][9][10], excellent biodegradability and biocompatibility [11], these stimulus-responsive polymers have shown great potential applications in biomedical devices [12][13][14], flexible electronics [15,16], smart robotics [17] and space deployable structures [18,19]. Among them, stimulus-responsive shape memory polymers (SMPs) have been extensively studied due to their tailorable dynamics with shape-memory effect (SME) and shape recovery behaviour [20,21].…”

Section: Introductionmentioning

confidence: 99%

An extended shoving model for dynamic fluctuation of glass transition in amorphous polymer towards cooperative shape-memory effect

Liu

Elmarakbi

et al. 2023

Proc. R. Soc. A.

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Phase transition theory is a powerful approach to study shape-memory effect (SME) and dynamic relaxation behaviour in amorphous shape-memory polymers (SMPs) undergoing glass transition processes. However, previous studies are mostly limited to building-up of experimental models, mainly due to poor understanding of dynamic fluctuation of glass transition. In this study, the dynamic fluctuation of glass transition in the amorphous SMPs, whose thermodynamic SME is governed by the phase transition theory, was investigated using a shoving model. A constitutive relationship between potential energy and cooperatively rearranging region (CRR) volume is firstly developed to explore the working principle of phase transition in the thermodynamic SME of amorphous SMPs, based on the shoving model and Adam–Gibbs domain size model. The effect of CRR volume on the phase transition is further formulated using the strain function, and then employed to develop a constitutive stress–strain equation based on the extended Maxwell model. The working principles of cooperative SME and dynamic relaxation behaviours have been explored and discussed. Finally, the effectiveness of the analytical results obtained using the proposed model has been verified using the experimental results of amorphous SMPs reported in literature.

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A robust polyacrylic acid/chitosan cryogel for rapid hemostasis

Cited by 21 publications

References 47 publications

Ultra‐Histocompatible and Electrophysiological‐Adapted PEDOT‐Based Hydrogels Designed for Cardiac Repair

Ultra‐Histocompatible and Electrophysiological‐Adapted PEDOT‐Based Hydrogels Designed for Cardiac Repair

An Intrapericardial Injectable Hydrogel Patch for Mechanical–Electrical Coupling with Infarcted Myocardium

An extended shoving model for dynamic fluctuation of glass transition in amorphous polymer towards cooperative shape-memory effect

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