A robust LC-MS/MS method to measure 8-oxoGuo, 8-oxodG, and NMN in human serum and urine

Zou, Yutong; Ma, Xiaoli; Tang, Yueming; Lin, Liling; Yu, Jialei; Zhong, Jianxin; Wang, Danchen; Cheng, Xinqi; Gao, Jing; Yu, Songlin; Qiu, Ling

doi:10.1016/j.ab.2022.114970

Analytical Biochemistry

2023

DOI: 10.1016/j.ab.2022.114970

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A robust LC-MS/MS method to measure 8-oxoGuo, 8-oxodG, and NMN in human serum and urine

Yutong Zou

Xiaoli Ma

Yueming Tang

et al.

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Cited by 3 publications

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“…We have established and applied the LC–MS/MS method for the determination of 8‐oxoG and 8‐oxodG in urine samples for several years, 3 and more than two researchers in our laboratory have validated the unnecessary to pre‐heat the frozen urine samples to release 8‐oxoG and 8‐oxodG from precipitations with this pretreatment methods. Moreover, the verification results including precision were deemed good with the total coefficient of variation <4.2% 4 …”

mentioning

confidence: 89%

“…Moreover, the verification results including precision were deemed good with the total coefficient of variation <4.2%. 4 …”

mentioning

confidence: 99%

“…Moreover, the verification results including precision were deemed good with the total coefficient of variation <4.2%. 4 (3) To obtain more accurate measurements, the fresh urine samples without precipitations are first recommended. In this study 1 and previous reports, 3,4 we all collected fresh urine samples, which are drawn at the same day; thus, there were no apparent precipitations in the urine samples before aliquoted and frozen.…”

mentioning

confidence: 99%

“…4 (3) To obtain more accurate measurements, the fresh urine samples without precipitations are first recommended. In this study 1 and previous reports, 3,4 we all collected fresh urine samples, which are drawn at the same day; thus, there were no apparent precipitations in the urine samples before aliquoted and frozen. the frozen urine samples to completely release the 8-oxoG and 8-oxodG from precipitations.…”

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confidence: 99%

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Reply to commentary on “Is pre‐heat necessary for the measurement of 8‐oxo‐7,8‐dihydroguanosine and 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine in urine samples”

Zou

et al. 2023

Clinical Laboratory Analysis

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mentioning

confidence: 89%

“…Moreover, the verification results including precision were deemed good with the total coefficient of variation <4.2%. 4 …”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Reply to commentary on “Is pre‐heat necessary for the measurement of 8‐oxo‐7,8‐dihydroguanosine and 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine in urine samples”

Zou

et al. 2023

Clinical Laboratory Analysis

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Development and validation of an LC-MS/MS method for quantifying NAD+ and related metabolites in mice sciatic nerves and its application to a nerve injury animal model

Ma,

Deng,

2024

Journal of Chromatography A

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Nightshift work can induce oxidative DNA damage: a pilot study

Zou

Chen

et al. 2023

BMC Public Health

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Background Regular sleep is very important for human health; however, the short-term and long-term effects of nightshift with sleep deprivation and disturbance on human metabolism, such as oxidative stress, have not been effectively evaluated based on a realistic cohort. We conducted the first long-term follow-up cohort study to evaluate the effect of nightshift work on DNA damage. Methods We recruited 16 healthy volunteers (aged 33 ± 5 years) working night shifts at the Department of Laboratory Medicine at a local hospital. Their matched serum and urine samples were collected at four time points: before, during (twice), and after the nightshift period. The levels of 8-oxo-7,8-dihydroguanosine (8-oxoG) and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG), two important nucleic-acid damage markers, were accurately determined based on a robust self-established LC‒MS/MS method. The Mann–Whitney U or Kruskal–Wallis test was used for comparisons, and Pearson’s or Spearman’s correlation analysis was used to calculate the correlation coefficients. Results The levels of serum 8-oxodG, estimated glomerular filtration rate-corrected serum 8-oxodG, and the serum-to-urine 8-oxodG ratio significantly increased during the nightshift period. These levels were significantly higher than pre-nightshift work level even after 1 month of discontinuation, but no such significant change was found for 8-oxoG. Moreover, 8-oxoG and 8-oxodG levels were significantly positively associated with many routine biomarkers, such as total bilirubin and urea levels, and significantly negatively associated with serum lipids, such as total cholesterol levels. Conclusion The results of our cohort study suggested that working night shifts may increase oxidative DNA damage even after a month of discontinuing nightshift work. Further studies with large-scale cohorts, different nightshift modes, and longer follow-up times are needed to clarify the short- and long-term effects of night shifts on DNA damage and find effective solutions to combat the negative effects.

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A robust LC-MS/MS method to measure 8-oxoGuo, 8-oxodG, and NMN in human serum and urine

Cited by 3 publications

References 42 publications

Reply to commentary on “Is pre‐heat necessary for the measurement of 8‐oxo‐7,8‐dihydroguanosine and 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine in urine samples”

Reply to commentary on “Is pre‐heat necessary for the measurement of 8‐oxo‐7,8‐dihydroguanosine and 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine in urine samples”

Development and validation of an LC-MS/MS method for quantifying NAD+ and related metabolites in mice sciatic nerves and its application to a nerve injury animal model

Nightshift work can induce oxidative DNA damage: a pilot study

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