A robust electrochemical immunosensor based on hydroxyl pillar[5]arene@AuNPs@g-C3N4 hybrid nanomaterial for ultrasensitive detection of prostate specific antigen

Zhou, Xu; Yang, Long; Tan, Xiaoping; Zhao, Genfu; Xie, Xiaoguang; Du, Guanben

doi:10.1016/j.bios.2018.04.036

Cited by 90 publications

(30 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gold is another widely used material for electrode modification and antibody immobilization because of its biocompatibility [32]. Zhou et al developed an electrochemical immunosensor based on antibody-immobilized AuNPs [33]. The transducer used in that study was composed of antibodies immobilized on the AuNP layer for the detection of PSA.…”

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

See 1 more Smart Citation

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

View full text Add to dashboard Cite

Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.

show abstract

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

“…Copyright (2020) John Wiley and Sons., Inc. (B) Immunosensor with non-covalently immobilized antibodies on AuNPs. Reproduced with permission from [33]. Copyright (2018) Elsevier.…”

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

View full text Add to dashboard Cite

show abstract

“…Also, on this fabricated immunosensor, gold nanoparticles (AuNPs) were adopted as effective nanomaterial for oriented immobilization of bioreceptors. AuNPs on the modified electrode can not only increase the amount of immobilized antibodies, but also speed up the electron transfer rate for signal amplification and thus increases the sensitivity of the designed immunosensor 37 , 38 . Therefore, the combination of NiFe-LDH/rGO nanocomposite and AuNPs should be a good immunosensing platform.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

Abolhasan

Khalilzadeh

Yousefi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

show abstract

“…A solution containing the PSA was added to the electrode surface, followed by the nanobioconjugates. At optimal conditions, the immunosensor was tested by DPV with different PSA target concentrations, having a linear response in the range from 0.0005 to 10.00 ng/mL, with a LOD of 0.12 pg/mL, see Figure 2 III [ 30 ].…”

Section: Nanobioconjugates Assemblymentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

View full text Add to dashboard Cite

Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

show abstract

A robust electrochemical immunosensor based on hydroxyl pillar[5]arene@AuNPs@g-C3N4 hybrid nanomaterial for ultrasensitive detection of prostate specific antigen

Cited by 90 publications

References 90 publications

Recent Progress in Electrochemical Immunosensors

Recent Progress in Electrochemical Immunosensors

Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Contact Info

Product

Resources

About