A roadmap for research in octoploid strawberry

Whitaker, Vance M.; Knapp, Steven J.; Hardigan, Michael A.; Edger, Patrick P.; Slovin, Janet P.; Bassil, Nahla V.; Hytönen, Timo; Mackenzie, Kathryn Kuligowska; Lee, Seong-Hee; Jung, Sook; Main, Dorrie; Barbey, Christopher R.; Verma, Sujeet

doi:10.1038/s41438-020-0252-1

Cited by 55 publications

(60 citation statements)

References 188 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, phenotypic selection for quantitative resistance to this pathogen has previously been the only solution (Bringhurst, Hansche, & Voth, 1968; Darrow, 1966; Maas et al., 1989; Shaw, Gubler, Larson, & Hansen, 1996, 2010). With recent advances in the development of genomic resources and supporting information (Edger et al., 2019; Hardigan et al., 2020; Whitaker et al., 2020), strawberry is well positioned for the application of genome‐informed breeding approaches (Crossa et al., 2017; Poland & Rutkoski, 2016). Here, we explore the prospects for increasing genetic gains and accelerating the development of Verticillium wilt resistant cultivars through the application of genomic selection, under the assumption that resistance to this pathogen is genetically complex in cultivated strawberry (Antanaviciute et al., 2015; Cockerton et al., 2019; Shaw et al., 1996; Vining et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

et al. 2020

Self Cite

View full text Add to dashboard Cite

Verticillium wilt, a soil-borne disease caused by the fungal pathogen Verticillium dahliae, threatens strawberry (Fragaria × ananassa) production worldwide. The development of resistant cultivars has been a persistent challenge, in part because the genetics of resistance is complex. The heritability of resistance and genetic gains in breeding for resistance to this pathogen have not been well documented. To elucidate the genetics, assess long-term genetic gains, and estimate the accuracy of genomic selection for resistance to Verticillium wilt, we analyzed a genetically diverse population of elite and exotic germplasm accessions (n = 984), including 245 cultivars developed since 1854. We observed a full range of phenotypes, from highly susceptible to highly resistant: < 3% were classified as highly resistant, whereas > 50% were classified as moderately to highly susceptible. Broad-sense heritability estimates ranged from 0.70-0.76, whereas narrow-sense genomic heritability estimates ranged from 0.33-0.45. We found that genetic gains in breeding for resistance to Verticillium wilt have been negative over the last 165 years (mean resistance has decreased over time). We identified several highly resistant accessions that might harbor favorable alleles that are either rare or non-existent in modern populations. We did not observe the segregation of large-effect loci. The accuracy of genomic predictions ranged from 0.38-0.53 among years and whole-genome regression methods. We show that genomic selection has promise for increasing genetic gains and accelerating the development of resistant cultivars in strawberry by shortening selection cycles and enabling selection in early developmental stages without phenotyping.

show abstract

Section: Introductionmentioning

confidence: 99%

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the diploid Fragaria vesca, the PF trait is caused by a recessive mutation in the SFL gene 31 . A major locus controlling PF in cultivated strawberry is the perpetual flowering and runnering (PFRU) locus, which modulates the balance between sexual and asexual plant reproduction 27,31 . FaPFRU shows opposite effects on flowering and runnering, exerting a positive effect on flowering and a negative effect on runnering, indicating that the two traits are genetically linked and share common physiological control 31 .…”

Section: Introductionmentioning

confidence: 99%

Woodland strawberry WRKY71 acts as a promoter of flowering via a transcriptional regulatory cascade

et al. 2020

View full text Add to dashboard Cite

The WRKY proteins are a large family of transcription factors that play important roles in stress responses and plant development. However, the roles of most WRKYs in strawberry are not well known. In this study, FvWRKY71 was isolated from the woodland strawberry 'Ruegen'. FvWRKY71 was highly expressed in the shoot apex and red fruit. Subcellular localization analysis showed that FvWRKY71 was located in the nucleus. Transactivation analysis showed that FvWRKY71 presented transcriptional activation activity in yeast. Overexpression of FvWRKY71 in Arabidopsis and woodland strawberry revealed early flowering in the transgenic plants compared with the wild-type control. Gene expression analysis indicated that the transcript levels of the flowering time and development integrator genes AP1, LFY, FT, AGL42, FUL, FPF1, SEP1, SEP2, and SEP3 were increased in FvWRKY71-overexpressing Arabidopsis and strawberry plants compared with the wild-type controls, which may result in accelerated flowering in transgenic plants. Furthermore, FvWRKY71 was proven to directly bind to the W-boxes (TTGACT/C) of the FvFUL, FvSEP1, FvAGL42, FvLFY, and FvFPF1 promoters in vitro and in vivo. Taken together, our results reveal a transcriptional regulatory cascade of FvWRKY71 involved in promoting flowering in woodland strawberry.

show abstract

“…Multiple resistance to a broad spectrum of diseases such as powdery mildew and leaf spot is still not available among commercial strawberry cultivars (i.e. human-selected clonal genotypes) 26 . Disease control is particularly challenging in strawberry production, since several cultivars present at the same time, owers, fruit and leaves, and are therefore subjected to a high risk of pesticide residue accumulation on berries 19 .…”

mentioning

confidence: 99%

WITHDRAWN: Genotype-Dependent Recruitment of the Strawberry Holobiome

Cellini¹,

Sangiorgio²,

Donati³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundCultivated strawberry (Fragaria × ananassa Duch., fam. Rosaceae) is an important fruit crop, greatly appreciated for its aroma and nutraceutical properties. Niche-specific characterisation of plant microbiome, from rhizosphere to aboveground plant organs, is crucial to understand the influence of structure and function of the microbial communities on plant phenotype, performances and disease resistance. Strawberry cultivation is challenged by a large variety of pathogens, which cause substantial economic losses and require the frequent application of pesticides. Biological control is a promising and safer alternative to the use of xenobiotic pesticides. Biological control agents isolated from the microbiome of the host plant may have a superior efficacy in comparison to non-indigenous microbial inoculants. Therefore, the characterization of the native microbiome along different plant compartments is a key step for the successful microbial manipulation in farmlands. Results Here, we provide the first comprehensive description of the soil, rhizosphere, root and aerial parts microbiome of three commercially important strawberry cultivars (‘Darselect’, ‘Elsanta’ and ‘Monterey’) under cultural conditions. The fungal and bacterial microbiomes were functionally characterised to investigate their influence on plant disease tolerance, plant mineral nutrient content and fruit quality. The core microbiome included 24 bacteria and 15 fungal operative taxon units which were present in all compartments and plant genotypes. However, both plant organ and genotype had a significant role in assembling the microbial communities. The microbial community assemblage across different soil and plant compartments significantly correlated with disease resistance, mineral nutrient content in the plant and with fruit quality parameters. Interestingly, only the disease tolerant genotype ‘Monterey’ was able to recruit Pseudomonas fluorescens in all plant organs and to establish symbiosis with the arbuscular mycorrhiza Rhizophagus irregularis. These two species include several strains acting as pathogen biocontrol agents, plant growth promoters and plant defence inducers. Conclusions Altogether, our study provides the first comprehensive view of strawberry microbiome in relation to plant genotype, health and nutritional status and fruit quality parameters, shedding light on potential practical applications to increase the sustainability of crop production.

show abstract

A roadmap for research in octoploid strawberry

Cited by 55 publications

References 188 publications

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

Accuracy of genomic selection and long‐term genetic gain for resistance to Verticillium wilt in strawberry

Woodland strawberry WRKY71 acts as a promoter of flowering via a transcriptional regulatory cascade

WITHDRAWN: Genotype-Dependent Recruitment of the Strawberry Holobiome

Contact Info

Product

Resources

About