A roadmap for intestinal regeneration

Quispe-Parra, David; Valentín, Griselle; García‐Arrarás, José E.

doi:10.1387/ijdb.200227dq

Cited by 19 publications

(24 citation statements)

References 62 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…with regeneration is the dedifferentiation of the mesentery cells 31 . Such dedifferentiation is usually associated with cellular reprogramming and the activation or inactivation of specific transcription factors (TFs).…”

Section: Differentially Expressed Transcription Factors (Detfs) One mentioning

confidence: 99%

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Quispe-Parra

Medina-Feliciano

Cruz-González

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 h and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.

show abstract

Section: Differentially Expressed Transcription Factors (Detfs) One mentioning

confidence: 99%

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Quispe-Parra

Medina-Feliciano

Cruz-González

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…This regulation, as in other animals, is probably carried out through apoptosis, ROS production, and myc expression [ 160 , 162 , 163 ]. In H. glaberrima , the suppression of myc via RNAi led to a decrease in cell proliferation [ 169 ]. Similar results were also obtained for A. japonicus [ 167 ].…”

Section: Regeneration After Posterior Eviscerationmentioning

confidence: 99%

Molecular Aspects of Regeneration Mechanisms in Holothurians

Dolmatov

2021

Genes

View full text Add to dashboard Cite

Holothurians, or sea cucumbers, belong to the phylum Echinodermata. They show good regenerative abilities. The present review provides an analysis of available data on the molecular aspects of regeneration mechanisms in holothurians. The genes and signaling pathways activated during the asexual reproduction and the formation of the anterior and posterior parts of the body, as well as the molecular mechanisms that provide regeneration of the nervous and digestive systems, are considered here. Damage causes a strong stress response, the signs of which are recorded even at late regeneration stages. In holothurian tissues, the concentrations of reactive oxygen species and antioxidant enzymes increase. Furthermore, the cellular and humoral components of the immune system are activated. Extracellular matrix remodeling and Wnt signaling play a major role in the regeneration in holothurians. All available morphological and molecular data show that the dedifferentiation of specialized cells in the remnant of the organ and the epithelial morphogenesis constitute the basis of regeneration in holothurians. However, depending on the type of damage, the mechanisms of regeneration may differ significantly in the spatial organization of regeneration process, the involvement of different cell types, and the depth of reprogramming of their genome (dedifferentiation or transdifferentiation).

show abstract

“…This is a natural process that can be induced in the lab by various methods. The new intestine regenerates from the tip of the mesentery to which the eviscerated intestine was attached, and the cellular events associated with the regeneration process have been well documented (Hyman, 1955;García-Arrarás and Greenberg, 2001;Mashanov and García-Arrarás, 2011;García-Arrarás et al, 2019;Quispe-Parra et al, (2021b)). These cellular events, except for some species-specific differences, appear to be shared by most species studied.…”

Section: Sea Cucumber Regenerationmentioning

confidence: 99%

Regeneration in Echinoderms: Molecular Advancements

Medina-Feliciano

García‐Arrarás

2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Which genes and gene signaling pathways mediate regenerative processes? In recent years, multiple studies, using a variety of animal models, have aimed to answer this question. Some answers have been obtained from transcriptomic and genomic studies where possible gene and gene pathway candidates thought to be involved in tissue and organ regeneration have been identified. Several of these studies have been done in echinoderms, an animal group that forms part of the deuterostomes along with vertebrates. Echinoderms, with their outstanding regenerative abilities, can provide important insights into the molecular basis of regeneration. Here we review the available data to determine the genes and signaling pathways that have been proposed to be involved in regenerative processes. Our analyses provide a curated list of genes and gene signaling pathways and match them with the different cellular processes of the regenerative response. In this way, the molecular basis of echinoderm regenerative potential is revealed, and is available for comparisons with other animal taxa.

show abstract

A roadmap for intestinal regeneration

Cited by 19 publications

References 62 publications

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Molecular Aspects of Regeneration Mechanisms in Holothurians

Regeneration in Echinoderms: Molecular Advancements

Contact Info

Product

Resources

About